Experiment: TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyaA and hemC are separated by 365 nucleotides hemC and hemD overlap by 4 nucleotides hemD and hemX are separated by 21 nucleotides
MCAODC_04950: cyaA - class I adenylate cyclase, at 998,793 to 1,001,339
cyaA
MCAODC_04955: hemC - hydroxymethylbilane synthase, at 1,001,705 to 1,002,667
hemC
MCAODC_04960: hemD - uroporphyrinogen-III synthase, at 1,002,664 to 1,003,404
hemD
MCAODC_04965: hemX - uroporphyrinogen-III C-methyltransferase, at 1,003,426 to 1,004,655
hemX
Position (kb)
1001
1002
1003 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1001.422 kb on - strand at 1001.540 kb on + strand at 1001.540 kb on + strand at 1001.544 kb on + strand at 1001.544 kb on + strand at 1001.544 kb on + strand at 1001.544 kb on + strand at 1001.544 kb on + strand at 1001.545 kb on - strand at 1001.545 kb on - strand at 1001.545 kb on - strand at 1001.545 kb on - strand at 1001.552 kb on + strand at 1001.552 kb on + strand at 1001.553 kb on - strand at 1001.553 kb on - strand at 1001.553 kb on - strand at 1001.553 kb on - strand at 1001.562 kb on + strand at 1001.562 kb on + strand at 1001.571 kb on + strand at 1001.583 kb on + strand at 1001.583 kb on + strand at 1001.584 kb on - strand at 1001.649 kb on + strand at 1001.649 kb on + strand at 1001.650 kb on - strand at 1001.690 kb on + strand at 1001.690 kb on + strand at 1001.709 kb on + strand at 1002.644 kb on + strand at 1002.644 kb on + strand at 1003.388 kb on + strand at 1003.388 kb on + strand at 1003.397 kb on + strand at 1003.397 kb on + strand at 1003.397 kb on + strand at 1003.397 kb on + strand at 1003.398 kb on - strand at 1003.398 kb on - strand at 1003.402 kb on + strand at 1003.402 kb on + strand at 1003.402 kb on + strand at 1003.402 kb on + strand at 1003.402 kb on + strand at 1003.402 kb on + strand at 1003.403 kb on - strand at 1003.518 kb on + strand at 1003.518 kb on + strand at 1003.561 kb on - strand, within hemX at 1003.588 kb on + strand, within hemX at 1003.588 kb on + strand, within hemX at 1003.589 kb on - strand, within hemX at 1003.589 kb on - strand, within hemX at 1003.589 kb on - strand, within hemX at 1003.600 kb on - strand, within hemX
Per-strain Table
Position Strand Gene LocusTag Fraction TP9 remove 1,001,422 - -0.3 1,001,540 + -0.1 1,001,540 + +0.3 1,001,544 + -1.7 1,001,544 + -0.1 1,001,544 + +0.6 1,001,544 + +1.3 1,001,544 + -0.1 1,001,545 - -0.9 1,001,545 - -1.4 1,001,545 - -0.0 1,001,545 - -1.0 1,001,552 + +1.5 1,001,552 + +1.3 1,001,553 - +0.3 1,001,553 - -0.3 1,001,553 - -0.0 1,001,553 - -0.5 1,001,562 + +0.3 1,001,562 + +0.4 1,001,571 + -0.5 1,001,583 + -0.1 1,001,583 + +0.1 1,001,584 - -0.1 1,001,649 + +0.3 1,001,649 + -0.6 1,001,650 - +0.7 1,001,690 + +0.4 1,001,690 + +0.6 1,001,709 + -2.2 1,002,644 + -0.6 1,002,644 + +0.9 1,003,388 + +1.1 1,003,388 + -0.8 1,003,397 + +2.7 1,003,397 + +0.1 1,003,397 + +0.3 1,003,397 + -2.7 1,003,398 - -0.7 1,003,398 - +2.1 1,003,402 + -0.1 1,003,402 + -0.1 1,003,402 + +0.9 1,003,402 + +0.0 1,003,402 + -0.7 1,003,402 + +0.2 1,003,403 - -0.1 1,003,518 + -1.3 1,003,518 + +0.0 1,003,561 - hemX MCAODC_04965 0.11 -0.4 1,003,588 + hemX MCAODC_04965 0.13 +0.9 1,003,588 + hemX MCAODC_04965 0.13 +1.9 1,003,589 - hemX MCAODC_04965 0.13 +2.9 1,003,589 - hemX MCAODC_04965 0.13 +0.1 1,003,589 - hemX MCAODC_04965 0.13 -0.2 1,003,600 - hemX MCAODC_04965 0.14 +0.3
Or see this region's nucleotide sequence