Experiment: Ying_Nucleobase14 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF57 and GFF58 overlap by 7 nucleotides GFF58 and GFF59 overlap by 4 nucleotides GFF59 and GFF60 are separated by 9 nucleotides GFF60 and GFF61 overlap by 4 nucleotides
GFF57 - hypothetical protein, at 58,306 to 58,716
GFF57
GFF58 - Tyrosine-protein kinase EpsD (EC 2.7.10.2), at 58,710 to 59,570
GFF58
GFF59 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 59,567 to 60,940
GFF59
GFF60 - Capsular polysaccharide biosynthesis/export periplasmic protein WcbA; Capsular polysaccharide export system protein KpsC, at 60,950 to 61,759
GFF60
GFF61 - hypothetical protein, at 61,756 to 62,721
GFF61
Position (kb) 59
60
61 Strain fitness (log2 ratio) -1
0
1
2 at 58.714 kb on + strand at 58.715 kb on - strand at 60.006 kb on + strand, within GFF59 at 60.006 kb on + strand, within GFF59 at 60.528 kb on + strand, within GFF59 at 60.529 kb on - strand, within GFF59 at 60.738 kb on + strand, within GFF59 at 60.825 kb on + strand at 61.173 kb on + strand, within GFF60 at 61.197 kb on + strand, within GFF60 at 61.198 kb on - strand, within GFF60 at 61.198 kb on - strand, within GFF60 at 61.221 kb on - strand, within GFF60 at 61.928 kb on + strand, within GFF61
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1 remove 58,714 + -1.1 58,715 - -0.8 60,006 + GFF59 0.32 -1.2 60,006 + GFF59 0.32 +1.1 60,528 + GFF59 0.70 +0.9 60,529 - GFF59 0.70 -1.5 60,738 + GFF59 0.85 +0.2 60,825 + -0.1 61,173 + GFF60 0.28 +0.5 61,197 + GFF60 0.30 -0.4 61,198 - GFF60 0.31 +1.8 61,198 - GFF60 0.31 +0.6 61,221 - GFF60 0.33 +1.5 61,928 + GFF61 0.18 -0.3
Or see this region's nucleotide sequence