Experiment: Ying_Nucleobase14 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5252 and GFF5253 are separated by 160 nucleotides GFF5253 and GFF5254 overlap by 4 nucleotides GFF5254 and GFF5255 are separated by 19 nucleotides
GFF5252 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 2,267,836 to 2,268,762
GFF5252
GFF5253 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,268,923 to 2,270,455
GFF5253
GFF5254 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 2,270,452 to 2,271,390
GFF5254
GFF5255 - ABC transporter substrate-binding protein, at 2,271,410 to 2,272,357
GFF5255
Position (kb)
2268
2269
2270
2271 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2268.040 kb on + strand, within GFF5252 at 2268.041 kb on - strand, within GFF5252 at 2268.041 kb on - strand, within GFF5252 at 2268.094 kb on + strand, within GFF5252 at 2268.094 kb on + strand, within GFF5252 at 2268.337 kb on + strand, within GFF5252 at 2268.337 kb on + strand, within GFF5252 at 2268.532 kb on + strand, within GFF5252 at 2268.533 kb on - strand, within GFF5252 at 2268.553 kb on + strand, within GFF5252 at 2268.656 kb on - strand, within GFF5252 at 2268.807 kb on + strand at 2268.808 kb on - strand at 2268.810 kb on + strand at 2268.824 kb on - strand at 2268.824 kb on - strand at 2268.829 kb on + strand at 2268.829 kb on + strand at 2268.830 kb on - strand at 2269.133 kb on + strand, within GFF5253 at 2269.134 kb on + strand, within GFF5253 at 2269.202 kb on + strand, within GFF5253 at 2269.202 kb on + strand, within GFF5253 at 2269.203 kb on - strand, within GFF5253 at 2269.203 kb on - strand, within GFF5253 at 2269.203 kb on - strand, within GFF5253 at 2269.203 kb on - strand, within GFF5253 at 2269.203 kb on - strand, within GFF5253 at 2269.307 kb on + strand, within GFF5253 at 2269.307 kb on + strand, within GFF5253 at 2269.308 kb on - strand, within GFF5253 at 2269.454 kb on + strand, within GFF5253 at 2269.455 kb on - strand, within GFF5253 at 2269.499 kb on + strand, within GFF5253 at 2269.533 kb on - strand, within GFF5253 at 2270.157 kb on - strand, within GFF5253 at 2270.384 kb on + strand at 2270.827 kb on + strand, within GFF5254 at 2270.828 kb on - strand, within GFF5254 at 2270.887 kb on + strand, within GFF5254 at 2270.887 kb on + strand, within GFF5254 at 2271.131 kb on - strand, within GFF5254 at 2271.389 kb on + strand at 2271.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1 remove 2,268,040 + GFF5252 0.22 +0.0 2,268,041 - GFF5252 0.22 +1.0 2,268,041 - GFF5252 0.22 -1.8 2,268,094 + GFF5252 0.28 +0.6 2,268,094 + GFF5252 0.28 -0.5 2,268,337 + GFF5252 0.54 -1.8 2,268,337 + GFF5252 0.54 +0.7 2,268,532 + GFF5252 0.75 -0.4 2,268,533 - GFF5252 0.75 +0.5 2,268,553 + GFF5252 0.77 +0.4 2,268,656 - GFF5252 0.88 +1.8 2,268,807 + -1.1 2,268,808 - -0.3 2,268,810 + -0.2 2,268,824 - -0.2 2,268,824 - -1.0 2,268,829 + +0.3 2,268,829 + +0.2 2,268,830 - +0.2 2,269,133 + GFF5253 0.14 -0.6 2,269,134 + GFF5253 0.14 -0.4 2,269,202 + GFF5253 0.18 +0.5 2,269,202 + GFF5253 0.18 -0.2 2,269,203 - GFF5253 0.18 +0.3 2,269,203 - GFF5253 0.18 -0.0 2,269,203 - GFF5253 0.18 +2.0 2,269,203 - GFF5253 0.18 -0.6 2,269,203 - GFF5253 0.18 -0.0 2,269,307 + GFF5253 0.25 -1.1 2,269,307 + GFF5253 0.25 +0.3 2,269,308 - GFF5253 0.25 +0.4 2,269,454 + GFF5253 0.35 -0.2 2,269,455 - GFF5253 0.35 +0.4 2,269,499 + GFF5253 0.38 +0.4 2,269,533 - GFF5253 0.40 +1.0 2,270,157 - GFF5253 0.80 +0.7 2,270,384 + -0.0 2,270,827 + GFF5254 0.40 -1.2 2,270,828 - GFF5254 0.40 -0.1 2,270,887 + GFF5254 0.46 +0.4 2,270,887 + GFF5254 0.46 +1.7 2,271,131 - GFF5254 0.72 -0.4 2,271,389 + +0.3 2,271,389 - +0.3
Or see this region's nucleotide sequence