Experiment: Ying_Nucleobase14 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3648 and GFF3649 are separated by 3 nucleotides GFF3649 and GFF3650 are separated by 63 nucleotides GFF3650 and GFF3651 are separated by 9 nucleotides
GFF3648 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 533,200 to 536,487
GFF3648
GFF3649 - Inositol-1-monophosphatase (EC 3.1.3.25), at 536,491 to 537,375
GFF3649
GFF3650 - tRNA:Cm32/Um32 methyltransferase, at 537,439 to 538,254
GFF3650
GFF3651 - Serine acetyltransferase (EC 2.3.1.30), at 538,264 to 539,034
GFF3651
Position (kb) 536
537
538 Strain fitness (log2 ratio) -1
0
1
2 at 535.541 kb on + strand, within GFF3648 at 535.542 kb on - strand, within GFF3648 at 535.542 kb on - strand, within GFF3648 at 535.542 kb on - strand, within GFF3648 at 535.542 kb on - strand, within GFF3648 at 535.606 kb on + strand, within GFF3648 at 535.606 kb on + strand, within GFF3648 at 535.610 kb on - strand, within GFF3648 at 535.853 kb on - strand, within GFF3648 at 536.212 kb on + strand at 536.212 kb on + strand at 536.213 kb on - strand at 536.272 kb on + strand at 536.272 kb on + strand at 536.272 kb on + strand at 536.272 kb on + strand at 537.498 kb on + strand at 537.901 kb on + strand, within GFF3650 at 537.902 kb on - strand, within GFF3650 at 537.902 kb on - strand, within GFF3650 at 538.252 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1 remove 535,541 + GFF3648 0.71 -0.2 535,542 - GFF3648 0.71 -0.8 535,542 - GFF3648 0.71 +1.1 535,542 - GFF3648 0.71 +0.4 535,542 - GFF3648 0.71 -0.3 535,606 + GFF3648 0.73 +0.0 535,606 + GFF3648 0.73 +1.7 535,610 - GFF3648 0.73 +0.2 535,853 - GFF3648 0.81 -0.2 536,212 + +0.0 536,212 + +0.6 536,213 - -0.8 536,272 + -0.0 536,272 + +0.7 536,272 + -0.3 536,272 + -1.4 537,498 + +0.8 537,901 + GFF3650 0.57 -0.6 537,902 - GFF3650 0.57 -1.5 537,902 - GFF3650 0.57 -0.5 538,252 + +2.3
Or see this region's nucleotide sequence