Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF3471

Experiment: Ying_Nucleobase14 rep C; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3470 and GFF3471 are separated by 27 nucleotidesGFF3471 and GFF3472 overlap by 4 nucleotidesGFF3472 and GFF3473 are separated by 30 nucleotides GFF3470 - Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-), at 350,926 to 352,593 GFF3470 GFF3471 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 352,621 to 353,802 GFF3471 GFF3472 - Enoyl-CoA hydratase (EC 4.2.1.17), at 353,799 to 354,599 GFF3472 GFF3473 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 354,630 to 355,412 GFF3473 Position (kb) 352 353 354Strain fitness (log2 ratio) -2 -1 0 1at 351.666 kb on - strand, within GFF3470at 351.771 kb on + strand, within GFF3470at 351.909 kb on - strand, within GFF3470at 352.269 kb on + strand, within GFF3470at 352.269 kb on + strand, within GFF3470at 352.449 kb on - strandat 352.839 kb on - strand, within GFF3471at 352.949 kb on - strand, within GFF3471at 353.063 kb on - strand, within GFF3471at 353.708 kb on - strandat 354.615 kb on + strandat 354.616 kb on - strandat 354.765 kb on - strand, within GFF3473at 354.765 kb on - strand, within GFF3473at 354.789 kb on + strand, within GFF3473

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1
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351,666 - GFF3470 0.44 -0.0
351,771 + GFF3470 0.51 -0.0
351,909 - GFF3470 0.59 +0.3
352,269 + GFF3470 0.81 -2.0
352,269 + GFF3470 0.81 +0.8
352,449 - -0.2
352,839 - GFF3471 0.18 +0.1
352,949 - GFF3471 0.28 -0.5
353,063 - GFF3471 0.37 -1.4
353,708 - +0.4
354,615 + +0.5
354,616 - -0.7
354,765 - GFF3473 0.17 +0.6
354,765 - GFF3473 0.17 +1.2
354,789 + GFF3473 0.20 +0.1

Or see this region's nucleotide sequence