Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rutA and rutB overlap by 4 nucleotides rutB and rutC are separated by 11 nucleotides rutC and rutD are separated by 50 nucleotides
MCAODC_24000: rutA - pyrimidine utilization protein A, at 1,528,328 to 1,529,419
rutA
MCAODC_24005: rutB - peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB, at 1,529,416 to 1,530,111
rutB
MCAODC_24010: rutC - pyrimidine utilization protein C, at 1,530,123 to 1,530,419
rutC
MCAODC_24015: rutD - pyrimidine utilization protein D, at 1,530,470 to 1,531,270
rutD
Position (kb) 1529
1530
1531 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1528.596 kb on + strand, within rutA at 1528.597 kb on - strand, within rutA at 1528.602 kb on + strand, within rutA at 1528.603 kb on - strand, within rutA at 1528.701 kb on - strand, within rutA at 1528.715 kb on - strand, within rutA at 1528.733 kb on + strand, within rutA at 1528.813 kb on + strand, within rutA at 1528.963 kb on + strand, within rutA at 1528.963 kb on + strand, within rutA at 1529.039 kb on + strand, within rutA at 1529.039 kb on + strand, within rutA at 1529.039 kb on + strand, within rutA at 1529.039 kb on + strand, within rutA at 1529.039 kb on + strand, within rutA at 1529.039 kb on + strand, within rutA at 1529.039 kb on + strand, within rutA at 1529.039 kb on + strand, within rutA at 1529.039 kb on + strand, within rutA at 1529.040 kb on - strand, within rutA at 1529.040 kb on - strand, within rutA at 1529.040 kb on - strand, within rutA at 1529.040 kb on - strand, within rutA at 1529.040 kb on - strand, within rutA at 1529.043 kb on + strand, within rutA at 1529.043 kb on + strand, within rutA at 1529.044 kb on - strand, within rutA at 1529.044 kb on - strand, within rutA at 1529.051 kb on - strand, within rutA at 1529.051 kb on - strand, within rutA at 1529.102 kb on + strand, within rutA at 1529.249 kb on + strand, within rutA at 1529.250 kb on - strand, within rutA at 1529.271 kb on + strand, within rutA at 1529.271 kb on + strand, within rutA at 1529.271 kb on + strand, within rutA at 1529.272 kb on - strand, within rutA at 1529.451 kb on + strand at 1529.452 kb on - strand at 1529.452 kb on - strand at 1529.504 kb on - strand, within rutB at 1529.525 kb on + strand, within rutB at 1529.526 kb on - strand, within rutB at 1529.650 kb on + strand, within rutB at 1529.650 kb on + strand, within rutB at 1529.650 kb on + strand, within rutB at 1529.650 kb on + strand, within rutB at 1529.651 kb on - strand, within rutB at 1529.651 kb on - strand, within rutB at 1529.651 kb on - strand, within rutB at 1529.877 kb on - strand, within rutB at 1530.140 kb on + strand at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.207 kb on + strand, within rutC at 1530.208 kb on - strand, within rutC at 1530.208 kb on - strand, within rutC at 1530.208 kb on - strand, within rutC at 1530.208 kb on - strand, within rutC at 1530.242 kb on + strand, within rutC at 1530.243 kb on - strand, within rutC at 1530.307 kb on + strand, within rutC at 1530.360 kb on - strand, within rutC at 1530.411 kb on - strand at 1530.451 kb on - strand at 1530.497 kb on + strand at 1530.514 kb on - strand at 1530.650 kb on + strand, within rutD at 1530.656 kb on - strand, within rutD at 1530.819 kb on + strand, within rutD at 1530.820 kb on - strand, within rutD at 1530.862 kb on - strand, within rutD at 1530.866 kb on + strand, within rutD at 1531.003 kb on - strand, within rutD at 1531.016 kb on - strand, within rutD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,528,596 + rutA MCAODC_24000 0.25 +0.3 1,528,597 - rutA MCAODC_24000 0.25 +1.0 1,528,602 + rutA MCAODC_24000 0.25 +1.7 1,528,603 - rutA MCAODC_24000 0.25 -1.0 1,528,701 - rutA MCAODC_24000 0.34 +1.7 1,528,715 - rutA MCAODC_24000 0.35 +0.1 1,528,733 + rutA MCAODC_24000 0.37 +0.5 1,528,813 + rutA MCAODC_24000 0.44 +1.3 1,528,963 + rutA MCAODC_24000 0.58 -1.1 1,528,963 + rutA MCAODC_24000 0.58 -0.3 1,529,039 + rutA MCAODC_24000 0.65 -0.9 1,529,039 + rutA MCAODC_24000 0.65 +1.7 1,529,039 + rutA MCAODC_24000 0.65 -2.0 1,529,039 + rutA MCAODC_24000 0.65 -0.3 1,529,039 + rutA MCAODC_24000 0.65 -2.9 1,529,039 + rutA MCAODC_24000 0.65 -0.6 1,529,039 + rutA MCAODC_24000 0.65 +0.5 1,529,039 + rutA MCAODC_24000 0.65 -0.5 1,529,039 + rutA MCAODC_24000 0.65 -0.1 1,529,040 - rutA MCAODC_24000 0.65 +1.4 1,529,040 - rutA MCAODC_24000 0.65 -1.9 1,529,040 - rutA MCAODC_24000 0.65 -1.1 1,529,040 - rutA MCAODC_24000 0.65 -0.1 1,529,040 - rutA MCAODC_24000 0.65 -0.3 1,529,043 + rutA MCAODC_24000 0.65 -0.4 1,529,043 + rutA MCAODC_24000 0.65 +0.2 1,529,044 - rutA MCAODC_24000 0.66 +1.6 1,529,044 - rutA MCAODC_24000 0.66 +0.9 1,529,051 - rutA MCAODC_24000 0.66 +0.4 1,529,051 - rutA MCAODC_24000 0.66 -0.4 1,529,102 + rutA MCAODC_24000 0.71 -0.7 1,529,249 + rutA MCAODC_24000 0.84 +0.0 1,529,250 - rutA MCAODC_24000 0.84 -0.1 1,529,271 + rutA MCAODC_24000 0.86 -1.6 1,529,271 + rutA MCAODC_24000 0.86 +0.9 1,529,271 + rutA MCAODC_24000 0.86 +0.4 1,529,272 - rutA MCAODC_24000 0.86 -0.6 1,529,451 + +0.7 1,529,452 - -1.0 1,529,452 - +0.8 1,529,504 - rutB MCAODC_24005 0.13 +0.1 1,529,525 + rutB MCAODC_24005 0.16 -1.0 1,529,526 - rutB MCAODC_24005 0.16 +0.3 1,529,650 + rutB MCAODC_24005 0.34 -0.5 1,529,650 + rutB MCAODC_24005 0.34 +0.4 1,529,650 + rutB MCAODC_24005 0.34 +0.0 1,529,650 + rutB MCAODC_24005 0.34 -0.6 1,529,651 - rutB MCAODC_24005 0.34 -1.2 1,529,651 - rutB MCAODC_24005 0.34 -0.4 1,529,651 - rutB MCAODC_24005 0.34 -1.6 1,529,877 - rutB MCAODC_24005 0.66 -0.3 1,530,140 + -0.0 1,530,207 + rutC MCAODC_24010 0.28 +3.3 1,530,207 + rutC MCAODC_24010 0.28 -2.3 1,530,207 + rutC MCAODC_24010 0.28 -0.3 1,530,207 + rutC MCAODC_24010 0.28 -0.3 1,530,207 + rutC MCAODC_24010 0.28 -0.3 1,530,207 + rutC MCAODC_24010 0.28 -0.3 1,530,207 + rutC MCAODC_24010 0.28 -0.3 1,530,207 + rutC MCAODC_24010 0.28 +1.0 1,530,207 + rutC MCAODC_24010 0.28 -0.4 1,530,207 + rutC MCAODC_24010 0.28 +1.7 1,530,207 + rutC MCAODC_24010 0.28 +0.1 1,530,208 - rutC MCAODC_24010 0.29 -1.5 1,530,208 - rutC MCAODC_24010 0.29 -1.1 1,530,208 - rutC MCAODC_24010 0.29 +1.7 1,530,208 - rutC MCAODC_24010 0.29 +0.7 1,530,242 + rutC MCAODC_24010 0.40 -0.2 1,530,243 - rutC MCAODC_24010 0.40 +1.7 1,530,307 + rutC MCAODC_24010 0.62 +1.7 1,530,360 - rutC MCAODC_24010 0.80 +0.7 1,530,411 - +1.2 1,530,451 - +0.0 1,530,497 + -1.4 1,530,514 - +0.0 1,530,650 + rutD MCAODC_24015 0.22 -0.9 1,530,656 - rutD MCAODC_24015 0.23 +1.7 1,530,819 + rutD MCAODC_24015 0.44 +0.1 1,530,820 - rutD MCAODC_24015 0.44 -1.3 1,530,862 - rutD MCAODC_24015 0.49 +1.9 1,530,866 + rutD MCAODC_24015 0.49 -0.3 1,531,003 - rutD MCAODC_24015 0.67 +2.2 1,531,016 - rutD MCAODC_24015 0.68 -2.6
Or see this region's nucleotide sequence