Strain Fitness in Escherichia coli ECRC101 around MCAODC_22415

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_22400 and ssb are separated by 10 nucleotidesssb and MCAODC_22410 overlap by 4 nucleotidesMCAODC_22410 and MCAODC_22415 are separated by 200 nucleotidesMCAODC_22415 and MCAODC_22420 overlap by 4 nucleotidesMCAODC_22420 and MCAODC_22425 are separated by 5 nucleotidesMCAODC_22425 and MCAODC_22430 overlap by 8 nucleotidesMCAODC_22430 and MCAODC_22435 overlap by 11 nucleotides MCAODC_22400: MCAODC_22400 - PerC family transcriptional regulator, at 1,241,882 to 1,242,154 _22400 MCAODC_22405: ssb - single-stranded DNA-binding protein, at 1,242,165 to 1,242,575 ssb MCAODC_22410: MCAODC_22410 - hypothetical protein, at 1,242,572 to 1,242,823 _22410 MCAODC_22415: MCAODC_22415 - DNA primase, at 1,243,024 to 1,244,424 _22415 MCAODC_22420: MCAODC_22420 - DUF4222 domain-containing protein, at 1,244,421 to 1,244,720 _22420 MCAODC_22425: MCAODC_22425 - hypothetical protein, at 1,244,726 to 1,244,959 _22425 MCAODC_22430: MCAODC_22430 - Putative prophage protein, at 1,244,952 to 1,245,416 _22430 MCAODC_22435: MCAODC_22435 - hypothetical protein, at 1,245,406 to 1,245,618 _22435 Position (kb) 1243 1244 1245Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1242.109 kb on - strand, within MCAODC_22400at 1242.109 kb on - strand, within MCAODC_22400at 1242.149 kb on + strandat 1242.150 kb on - strandat 1242.150 kb on - strandat 1242.177 kb on - strandat 1242.177 kb on - strandat 1242.279 kb on + strand, within ssbat 1242.279 kb on + strand, within ssbat 1242.611 kb on + strand, within MCAODC_22410at 1242.787 kb on - strand, within MCAODC_22410at 1242.901 kb on - strandat 1242.948 kb on + strandat 1242.948 kb on + strandat 1242.949 kb on - strandat 1242.949 kb on - strandat 1242.950 kb on + strandat 1243.037 kb on - strandat 1243.037 kb on - strandat 1243.083 kb on - strandat 1243.775 kb on - strand, within MCAODC_22415at 1243.793 kb on - strand, within MCAODC_22415at 1243.793 kb on - strand, within MCAODC_22415at 1243.793 kb on - strand, within MCAODC_22415at 1243.815 kb on - strand, within MCAODC_22415at 1243.903 kb on - strand, within MCAODC_22415at 1243.903 kb on - strand, within MCAODC_22415at 1243.904 kb on + strand, within MCAODC_22415at 1243.922 kb on + strand, within MCAODC_22415at 1243.922 kb on + strand, within MCAODC_22415at 1243.923 kb on - strand, within MCAODC_22415at 1243.923 kb on - strand, within MCAODC_22415at 1243.923 kb on - strand, within MCAODC_22415at 1243.924 kb on + strand, within MCAODC_22415at 1243.940 kb on - strand, within MCAODC_22415at 1243.951 kb on - strand, within MCAODC_22415at 1243.960 kb on - strand, within MCAODC_22415at 1244.137 kb on + strand, within MCAODC_22415at 1244.173 kb on - strand, within MCAODC_22415at 1244.176 kb on - strand, within MCAODC_22415at 1244.176 kb on - strand, within MCAODC_22415at 1244.204 kb on + strand, within MCAODC_22415at 1244.390 kb on + strandat 1244.504 kb on + strand, within MCAODC_22420at 1244.521 kb on - strand, within MCAODC_22420at 1244.569 kb on + strand, within MCAODC_22420at 1244.569 kb on + strand, within MCAODC_22420at 1244.596 kb on - strand, within MCAODC_22420at 1244.619 kb on - strand, within MCAODC_22420at 1244.987 kb on + strandat 1245.168 kb on - strand, within MCAODC_22430at 1245.219 kb on - strand, within MCAODC_22430at 1245.320 kb on + strand, within MCAODC_22430at 1245.320 kb on + strand, within MCAODC_22430at 1245.321 kb on - strand, within MCAODC_22430at 1245.321 kb on - strand, within MCAODC_22430at 1245.321 kb on - strand, within MCAODC_22430at 1245.321 kb on - strand, within MCAODC_22430at 1245.417 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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1,242,109 - MCAODC_22400 0.83 +3.5
1,242,109 - MCAODC_22400 0.83 +0.4
1,242,149 + +1.5
1,242,150 - +2.0
1,242,150 - -0.7
1,242,177 - -0.5
1,242,177 - +1.8
1,242,279 + ssb MCAODC_22405 0.28 +1.8
1,242,279 + ssb MCAODC_22405 0.28 +0.2
1,242,611 + MCAODC_22410 0.15 -0.5
1,242,787 - MCAODC_22410 0.85 -1.4
1,242,901 - +1.1
1,242,948 + +1.2
1,242,948 + +0.3
1,242,949 - -0.4
1,242,949 - +0.1
1,242,950 + -0.3
1,243,037 - -0.4
1,243,037 - +2.6
1,243,083 - +0.3
1,243,775 - MCAODC_22415 0.54 -0.7
1,243,793 - MCAODC_22415 0.55 -0.9
1,243,793 - MCAODC_22415 0.55 +0.6
1,243,793 - MCAODC_22415 0.55 -1.4
1,243,815 - MCAODC_22415 0.56 -0.9
1,243,903 - MCAODC_22415 0.63 -0.7
1,243,903 - MCAODC_22415 0.63 -0.7
1,243,904 + MCAODC_22415 0.63 +1.6
1,243,922 + MCAODC_22415 0.64 +0.1
1,243,922 + MCAODC_22415 0.64 -0.7
1,243,923 - MCAODC_22415 0.64 -0.4
1,243,923 - MCAODC_22415 0.64 +0.1
1,243,923 - MCAODC_22415 0.64 -0.1
1,243,924 + MCAODC_22415 0.64 -1.2
1,243,940 - MCAODC_22415 0.65 -1.2
1,243,951 - MCAODC_22415 0.66 -1.4
1,243,960 - MCAODC_22415 0.67 -1.5
1,244,137 + MCAODC_22415 0.79 +0.6
1,244,173 - MCAODC_22415 0.82 -0.9
1,244,176 - MCAODC_22415 0.82 -1.4
1,244,176 - MCAODC_22415 0.82 +0.1
1,244,204 + MCAODC_22415 0.84 -0.2
1,244,390 + +1.4
1,244,504 + MCAODC_22420 0.28 -1.9
1,244,521 - MCAODC_22420 0.33 -0.5
1,244,569 + MCAODC_22420 0.49 -0.9
1,244,569 + MCAODC_22420 0.49 -0.9
1,244,596 - MCAODC_22420 0.58 +2.6
1,244,619 - MCAODC_22420 0.66 +1.6
1,244,987 + -0.4
1,245,168 - MCAODC_22430 0.46 -2.2
1,245,219 - MCAODC_22430 0.57 -0.4
1,245,320 + MCAODC_22430 0.79 -0.7
1,245,320 + MCAODC_22430 0.79 +3.2
1,245,321 - MCAODC_22430 0.79 -0.9
1,245,321 - MCAODC_22430 0.79 +0.6
1,245,321 - MCAODC_22430 0.79 -1.4
1,245,321 - MCAODC_22430 0.79 -0.9
1,245,417 - +0.1

Or see this region's nucleotide sequence