Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt umuC and umuD overlap by 1 nucleotides umuD and hlyE are separated by 372 nucleotides hlyE and ycgN are separated by 206 nucleotides ycgN and ycgM are separated by 91 nucleotides
MCAODC_21905: umuC - DNA polymerase V catalytic protein, at 1,166,739 to 1,168,007
umuC
MCAODC_21910: umuD - Protein UmuD, at 1,168,007 to 1,168,426
umuD
MCAODC_21920: hlyE - hemolysin HlyE, at 1,168,799 to 1,169,710
hlyE
MCAODC_21925: ycgN - YcgN family cysteine cluster protein, at 1,169,917 to 1,170,363
ycgN
MCAODC_21930: ycgM - fumarylacetoacetate hydrolase family protein, at 1,170,455 to 1,171,114
ycgM
Position (kb)
1168
1169
1170 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1167.988 kb on - strand at 1168.058 kb on - strand, within umuD at 1168.058 kb on - strand, within umuD at 1168.105 kb on + strand, within umuD at 1168.145 kb on - strand, within umuD at 1168.229 kb on - strand, within umuD at 1168.247 kb on - strand, within umuD at 1168.272 kb on + strand, within umuD at 1168.272 kb on + strand, within umuD at 1168.426 kb on + strand at 1168.430 kb on - strand at 1168.547 kb on - strand at 1169.732 kb on + strand at 1169.741 kb on + strand at 1169.806 kb on - strand at 1169.880 kb on - strand at 1169.947 kb on - strand at 1170.004 kb on - strand, within ycgN at 1170.004 kb on - strand, within ycgN at 1170.104 kb on - strand, within ycgN at 1170.265 kb on - strand, within ycgN at 1170.352 kb on + strand at 1170.353 kb on - strand at 1170.353 kb on - strand at 1170.384 kb on + strand at 1170.396 kb on + strand at 1170.396 kb on + strand at 1170.396 kb on + strand at 1170.397 kb on - strand at 1170.397 kb on - strand at 1170.397 kb on - strand at 1170.453 kb on + strand at 1170.589 kb on + strand, within ycgM at 1170.590 kb on - strand, within ycgM at 1170.590 kb on - strand, within ycgM at 1170.634 kb on + strand, within ycgM
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,167,988 - +1.6 1,168,058 - umuD MCAODC_21910 0.12 +0.7 1,168,058 - umuD MCAODC_21910 0.12 +0.6 1,168,105 + umuD MCAODC_21910 0.23 +0.1 1,168,145 - umuD MCAODC_21910 0.33 +1.0 1,168,229 - umuD MCAODC_21910 0.53 -1.1 1,168,247 - umuD MCAODC_21910 0.57 +0.1 1,168,272 + umuD MCAODC_21910 0.63 -1.1 1,168,272 + umuD MCAODC_21910 0.63 +1.7 1,168,426 + +1.0 1,168,430 - +0.1 1,168,547 - -0.3 1,169,732 + +1.1 1,169,741 + -0.3 1,169,806 - +0.7 1,169,880 - -1.1 1,169,947 - -0.3 1,170,004 - ycgN MCAODC_21925 0.19 +0.9 1,170,004 - ycgN MCAODC_21925 0.19 -0.3 1,170,104 - ycgN MCAODC_21925 0.42 -0.7 1,170,265 - ycgN MCAODC_21925 0.78 -1.9 1,170,352 + -0.6 1,170,353 - +1.7 1,170,353 - +1.7 1,170,384 + +0.9 1,170,396 + -1.1 1,170,396 + +0.5 1,170,396 + -0.1 1,170,397 - +2.1 1,170,397 - +0.9 1,170,397 - -0.2 1,170,453 + -1.2 1,170,589 + ycgM MCAODC_21930 0.20 -1.5 1,170,590 - ycgM MCAODC_21930 0.20 +0.4 1,170,590 - ycgM MCAODC_21930 0.20 +0.1 1,170,634 + ycgM MCAODC_21930 0.27 +1.7
Or see this region's nucleotide sequence