Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_21185 and zorO are separated by 109 nucleotides zorO and zorP are separated by 519 nucleotides zorP and yehL are separated by 432 nucleotides
MCAODC_21185: MCAODC_21185 - Protein, at 1,042,580 to 1,042,897
_21185
MCAODC_21190: zorO - Small toxic protein ZorO, at 1,043,007 to 1,043,096
zorO
MCAODC_21200: zorP - Small toxic protein ZorP, at 1,043,616 to 1,043,705
zorP
MCAODC_21210: yehL - Uncharacterized protein YehL, at 1,044,138 to 1,045,226
yehL
Position (kb)
1043
1044 Strain fitness (log2 ratio)
-1
0
1
2 at 1042.666 kb on + strand, within MCAODC_21185 at 1042.681 kb on - strand, within MCAODC_21185 at 1042.786 kb on + strand, within MCAODC_21185 at 1042.788 kb on + strand, within MCAODC_21185 at 1042.949 kb on + strand at 1042.986 kb on - strand at 1043.074 kb on - strand, within zorO at 1043.324 kb on - strand at 1043.536 kb on - strand at 1043.652 kb on + strand, within zorP at 1043.664 kb on + strand, within zorP at 1043.665 kb on - strand, within zorP at 1043.771 kb on + strand at 1043.771 kb on + strand at 1043.775 kb on + strand at 1043.775 kb on + strand at 1043.853 kb on - strand at 1043.853 kb on - strand at 1043.890 kb on - strand at 1043.907 kb on - strand at 1043.935 kb on - strand at 1044.135 kb on + strand at 1044.136 kb on - strand at 1044.185 kb on - strand at 1044.250 kb on + strand, within yehL at 1044.275 kb on + strand, within yehL at 1044.309 kb on - strand, within yehL at 1044.321 kb on - strand, within yehL at 1044.341 kb on + strand, within yehL at 1044.421 kb on + strand, within yehL at 1044.434 kb on + strand, within yehL at 1044.476 kb on + strand, within yehL at 1044.480 kb on - strand, within yehL at 1044.510 kb on + strand, within yehL at 1044.511 kb on - strand, within yehL at 1044.511 kb on - strand, within yehL at 1044.646 kb on + strand, within yehL at 1044.647 kb on - strand, within yehL at 1044.651 kb on - strand, within yehL
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,042,666 + MCAODC_21185 0.27 -0.9 1,042,681 - MCAODC_21185 0.32 +0.0 1,042,786 + MCAODC_21185 0.65 +1.2 1,042,788 + MCAODC_21185 0.65 -0.7 1,042,949 + -0.7 1,042,986 - -0.1 1,043,074 - zorO MCAODC_21190 0.74 +1.3 1,043,324 - -1.1 1,043,536 - -1.4 1,043,652 + zorP MCAODC_21200 0.40 +0.0 1,043,664 + zorP MCAODC_21200 0.53 +0.1 1,043,665 - zorP MCAODC_21200 0.54 -1.2 1,043,771 + -1.1 1,043,771 + +0.4 1,043,775 + +1.7 1,043,775 + -1.3 1,043,853 - +0.6 1,043,853 - +1.7 1,043,890 - +1.7 1,043,907 - -0.4 1,043,935 - +0.7 1,044,135 + -0.6 1,044,136 - +0.2 1,044,185 - +0.4 1,044,250 + yehL MCAODC_21210 0.10 -0.9 1,044,275 + yehL MCAODC_21210 0.13 -0.6 1,044,309 - yehL MCAODC_21210 0.16 -0.1 1,044,321 - yehL MCAODC_21210 0.17 -0.3 1,044,341 + yehL MCAODC_21210 0.19 +1.3 1,044,421 + yehL MCAODC_21210 0.26 +0.0 1,044,434 + yehL MCAODC_21210 0.27 +1.2 1,044,476 + yehL MCAODC_21210 0.31 -0.5 1,044,480 - yehL MCAODC_21210 0.31 +0.3 1,044,510 + yehL MCAODC_21210 0.34 +0.1 1,044,511 - yehL MCAODC_21210 0.34 +2.3 1,044,511 - yehL MCAODC_21210 0.34 +0.3 1,044,646 + yehL MCAODC_21210 0.47 +1.2 1,044,647 - yehL MCAODC_21210 0.47 +0.7 1,044,651 - yehL MCAODC_21210 0.47 +0.7
Or see this region's nucleotide sequence