Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_20860 and MCAODC_20865 are separated by 66 nucleotides MCAODC_20865 and MCAODC_20870 overlap by 8 nucleotides MCAODC_20870 and lysC overlap by 38 nucleotides lysC and lysB overlap by 14 nucleotides lysB and lysA overlap by 13 nucleotides lysA and MCAODC_20890 are separated by 14 nucleotides
MCAODC_20860: MCAODC_20860 - Spike protein, at 982,538 to 983,173
_20860
MCAODC_20865: MCAODC_20865 - phage virion morphogenesis protein, at 983,240 to 983,692
_20865
MCAODC_20870: MCAODC_20870 - Tail completion protein R, at 983,685 to 984,152
_20870
MCAODC_20875: lysC - Rz1-like lysis system protein LysC, at 984,115 to 984,273
lysC
MCAODC_20880: lysB - Rz-like lysis system protein LysB, at 984,260 to 984,685
lysB
MCAODC_20885: lysA - Putative antiholin, at 984,673 to 985,098
lysA
MCAODC_20890: MCAODC_20890 - Endolysin, at 985,113 to 985,610
_20890
Position (kb) 983
984
985 Strain fitness (log2 ratio) -2
-1
0
1
2 at 982.801 kb on + strand, within MCAODC_20860 at 982.839 kb on + strand, within MCAODC_20860 at 982.917 kb on + strand, within MCAODC_20860 at 983.241 kb on + strand at 983.242 kb on - strand at 983.242 kb on - strand at 983.263 kb on + strand at 983.322 kb on + strand, within MCAODC_20865 at 983.462 kb on - strand, within MCAODC_20865 at 983.465 kb on - strand, within MCAODC_20865 at 983.720 kb on + strand at 983.721 kb on - strand at 983.721 kb on - strand at 983.771 kb on + strand, within MCAODC_20870 at 983.844 kb on - strand, within MCAODC_20870 at 984.010 kb on + strand, within MCAODC_20870 at 984.054 kb on - strand, within MCAODC_20870 at 984.054 kb on - strand, within MCAODC_20870 at 984.072 kb on + strand, within MCAODC_20870 at 984.084 kb on - strand, within MCAODC_20870 at 984.174 kb on + strand, within lysC at 984.174 kb on + strand, within lysC at 984.174 kb on + strand, within lysC at 984.175 kb on - strand, within lysC at 984.184 kb on + strand, within lysC at 984.239 kb on - strand, within lysC at 984.410 kb on + strand, within lysB at 984.720 kb on + strand, within lysA at 984.721 kb on - strand, within lysA at 984.724 kb on + strand, within lysA at 984.725 kb on - strand, within lysA at 984.725 kb on - strand, within lysA at 984.925 kb on + strand, within lysA at 984.925 kb on + strand, within lysA at 984.926 kb on - strand, within lysA at 984.929 kb on + strand, within lysA at 984.929 kb on + strand, within lysA at 984.930 kb on - strand, within lysA at 984.930 kb on - strand, within lysA at 984.941 kb on + strand, within lysA at 984.942 kb on - strand, within lysA at 984.942 kb on - strand, within lysA at 984.973 kb on - strand, within lysA at 984.973 kb on - strand, within lysA at 985.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 982,801 + MCAODC_20860 0.41 -0.2 982,839 + MCAODC_20860 0.47 +0.3 982,917 + MCAODC_20860 0.60 +0.8 983,241 + +0.9 983,242 - -0.4 983,242 - +0.2 983,263 + +1.7 983,322 + MCAODC_20865 0.18 -0.6 983,462 - MCAODC_20865 0.49 +1.7 983,465 - MCAODC_20865 0.50 +0.4 983,720 + +1.4 983,721 - -1.5 983,721 - +0.4 983,771 + MCAODC_20870 0.18 -0.2 983,844 - MCAODC_20870 0.34 -0.7 984,010 + MCAODC_20870 0.69 +0.7 984,054 - MCAODC_20870 0.79 +1.7 984,054 - MCAODC_20870 0.79 +1.2 984,072 + MCAODC_20870 0.83 +2.1 984,084 - MCAODC_20870 0.85 -1.0 984,174 + lysC MCAODC_20875 0.37 -2.1 984,174 + lysC MCAODC_20875 0.37 -0.4 984,174 + lysC MCAODC_20875 0.37 -0.4 984,175 - lysC MCAODC_20875 0.38 +0.4 984,184 + lysC MCAODC_20875 0.43 -0.6 984,239 - lysC MCAODC_20875 0.78 -0.3 984,410 + lysB MCAODC_20880 0.35 +0.9 984,720 + lysA MCAODC_20885 0.11 -1.6 984,721 - lysA MCAODC_20885 0.11 -0.6 984,724 + lysA MCAODC_20885 0.12 +1.2 984,725 - lysA MCAODC_20885 0.12 +0.7 984,725 - lysA MCAODC_20885 0.12 -1.0 984,925 + lysA MCAODC_20885 0.59 +1.4 984,925 + lysA MCAODC_20885 0.59 -1.1 984,926 - lysA MCAODC_20885 0.59 +0.9 984,929 + lysA MCAODC_20885 0.60 -0.7 984,929 + lysA MCAODC_20885 0.60 -0.1 984,930 - lysA MCAODC_20885 0.60 +0.6 984,930 - lysA MCAODC_20885 0.60 -0.3 984,941 + lysA MCAODC_20885 0.63 +0.9 984,942 - lysA MCAODC_20885 0.63 +0.0 984,942 - lysA MCAODC_20885 0.63 -0.1 984,973 - lysA MCAODC_20885 0.70 -1.5 984,973 - lysA MCAODC_20885 0.70 -0.7 985,114 + -0.2
Or see this region's nucleotide sequence