Strain Fitness in Escherichia coli ECRC101 around MCAODC_20520

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzx and wcaA overlap by 14 nucleotideswcaA and wzy overlap by 32 nucleotides MCAODC_20515: wzx - O157 family O-antigen flippase, at 911,108 to 912,499 wzx MCAODC_20520: wcaA - glycosyl transferase, at 912,486 to 913,232 wcaA MCAODC_20525: wzy - O157 family O-antigen polymerase, at 913,201 to 914,385 wzy Position (kb) 912 913 914Strain fitness (log2 ratio) -2 -1 0 1at 913.500 kb on - strand, within wzyat 913.677 kb on - strand, within wzyat 913.699 kb on + strand, within wzyat 913.735 kb on - strand, within wzyat 913.820 kb on - strand, within wzyat 914.095 kb on - strand, within wzyat 914.137 kb on + strand, within wzy

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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913,500 - wzy MCAODC_20525 0.25 -0.8
913,677 - wzy MCAODC_20525 0.40 +1.7
913,699 + wzy MCAODC_20525 0.42 -0.6
913,735 - wzy MCAODC_20525 0.45 -1.1
913,820 - wzy MCAODC_20525 0.52 -2.0
914,095 - wzy MCAODC_20525 0.75 -2.4
914,137 + wzy MCAODC_20525 0.79 -0.8

Or see this region's nucleotide sequence