Strain Fitness in Escherichia coli ECRC101 around MCAODC_20515

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfbE and wzx are separated by 2 nucleotideswzx and wcaA overlap by 14 nucleotideswcaA and wzy overlap by 32 nucleotides MCAODC_20510: rfbE - GDP-perosamine synthase RfbE/PerA, at 910,011 to 911,105 rfbE MCAODC_20515: wzx - O157 family O-antigen flippase, at 911,108 to 912,499 wzx MCAODC_20520: wcaA - glycosyl transferase, at 912,486 to 913,232 wcaA MCAODC_20525: wzy - O157 family O-antigen polymerase, at 913,201 to 914,385 wzy Position (kb) 911 912 913Strain fitness (log2 ratio) -1 0 1at 910.248 kb on - strand, within rfbEat 910.855 kb on - strand, within rfbEat 911.124 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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910,248 - rfbE MCAODC_20510 0.22 -0.6
910,855 - rfbE MCAODC_20510 0.77 -0.3
911,124 - -0.8

Or see this region's nucleotide sequence