Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaK and yeaL are separated by 274 nucleotides yeaL and nimR overlap by 44 nucleotides nimR and nimT are separated by 99 nucleotides
MCAODC_18720: yeaK - mischarged aminoacyl-tRNA deacylase, at 604,447 to 604,950
yeaK
MCAODC_18725: yeaL - UPF0756 membrane protein CKO_01811, at 605,225 to 605,671
yeaL
MCAODC_18730: nimR - DNA-binding transcriptional regulator NimR, at 605,628 to 606,446
nimR
MCAODC_18735: nimT - 2-nitroimidazole transporter, at 606,546 to 607,727
nimT
Position (kb) 605
606
607 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 604.634 kb on + strand, within yeaK at 604.701 kb on + strand, within yeaK at 604.702 kb on - strand, within yeaK at 604.952 kb on + strand at 604.952 kb on + strand at 604.952 kb on + strand at 605.055 kb on + strand at 605.056 kb on - strand at 605.185 kb on - strand at 605.376 kb on + strand, within yeaL at 605.630 kb on - strand at 605.670 kb on - strand at 605.670 kb on - strand at 605.684 kb on + strand at 605.685 kb on - strand at 605.724 kb on - strand, within nimR at 605.726 kb on + strand, within nimR at 605.727 kb on - strand, within nimR at 605.727 kb on - strand, within nimR at 605.750 kb on - strand, within nimR at 605.750 kb on - strand, within nimR at 605.976 kb on - strand, within nimR at 606.102 kb on + strand, within nimR at 606.103 kb on - strand, within nimR at 606.143 kb on - strand, within nimR at 606.222 kb on - strand, within nimR at 606.222 kb on - strand, within nimR at 606.222 kb on - strand, within nimR at 606.291 kb on - strand, within nimR at 606.297 kb on - strand, within nimR at 606.468 kb on + strand at 606.469 kb on - strand at 606.497 kb on - strand at 606.517 kb on - strand at 606.546 kb on - strand at 606.546 kb on - strand at 606.617 kb on + strand at 606.618 kb on - strand at 606.704 kb on - strand, within nimT at 606.704 kb on - strand, within nimT at 606.704 kb on - strand, within nimT at 606.786 kb on - strand, within nimT at 606.849 kb on + strand, within nimT at 606.854 kb on - strand, within nimT at 606.895 kb on + strand, within nimT at 606.900 kb on - strand, within nimT at 606.907 kb on + strand, within nimT at 606.923 kb on + strand, within nimT at 606.966 kb on + strand, within nimT at 607.013 kb on - strand, within nimT at 607.151 kb on - strand, within nimT at 607.151 kb on - strand, within nimT at 607.151 kb on - strand, within nimT at 607.151 kb on - strand, within nimT at 607.154 kb on + strand, within nimT at 607.154 kb on + strand, within nimT at 607.225 kb on - strand, within nimT at 607.228 kb on - strand, within nimT at 607.236 kb on - strand, within nimT at 607.315 kb on + strand, within nimT at 607.354 kb on + strand, within nimT
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 604,634 + yeaK MCAODC_18720 0.37 -0.5 604,701 + yeaK MCAODC_18720 0.50 -0.4 604,702 - yeaK MCAODC_18720 0.51 +0.9 604,952 + +1.2 604,952 + -0.4 604,952 + +2.0 605,055 + +2.1 605,056 - +2.6 605,185 - -0.7 605,376 + yeaL MCAODC_18725 0.34 +1.1 605,630 - +0.6 605,670 - -0.1 605,670 - +1.0 605,684 + +0.6 605,685 - -0.0 605,724 - nimR MCAODC_18730 0.12 +1.6 605,726 + nimR MCAODC_18730 0.12 +2.6 605,727 - nimR MCAODC_18730 0.12 -0.7 605,727 - nimR MCAODC_18730 0.12 +2.0 605,750 - nimR MCAODC_18730 0.15 +0.1 605,750 - nimR MCAODC_18730 0.15 -1.5 605,976 - nimR MCAODC_18730 0.42 +2.6 606,102 + nimR MCAODC_18730 0.58 -1.2 606,103 - nimR MCAODC_18730 0.58 -0.1 606,143 - nimR MCAODC_18730 0.63 +0.6 606,222 - nimR MCAODC_18730 0.73 -0.6 606,222 - nimR MCAODC_18730 0.73 -1.2 606,222 - nimR MCAODC_18730 0.73 +0.0 606,291 - nimR MCAODC_18730 0.81 -0.4 606,297 - nimR MCAODC_18730 0.82 -0.4 606,468 + -1.2 606,469 - -1.7 606,497 - +0.6 606,517 - +0.6 606,546 - -0.7 606,546 - -0.4 606,617 + -2.5 606,618 - +1.6 606,704 - nimT MCAODC_18735 0.13 +0.0 606,704 - nimT MCAODC_18735 0.13 +2.2 606,704 - nimT MCAODC_18735 0.13 +1.9 606,786 - nimT MCAODC_18735 0.20 +1.6 606,849 + nimT MCAODC_18735 0.26 -1.7 606,854 - nimT MCAODC_18735 0.26 -2.7 606,895 + nimT MCAODC_18735 0.30 +0.3 606,900 - nimT MCAODC_18735 0.30 -0.9 606,907 + nimT MCAODC_18735 0.31 -0.7 606,923 + nimT MCAODC_18735 0.32 +4.2 606,966 + nimT MCAODC_18735 0.36 -1.0 607,013 - nimT MCAODC_18735 0.40 +0.6 607,151 - nimT MCAODC_18735 0.51 +0.1 607,151 - nimT MCAODC_18735 0.51 -0.7 607,151 - nimT MCAODC_18735 0.51 -0.7 607,151 - nimT MCAODC_18735 0.51 +0.4 607,154 + nimT MCAODC_18735 0.51 +3.2 607,154 + nimT MCAODC_18735 0.51 +0.6 607,225 - nimT MCAODC_18735 0.57 +0.8 607,228 - nimT MCAODC_18735 0.58 +2.4 607,236 - nimT MCAODC_18735 0.58 +0.3 607,315 + nimT MCAODC_18735 0.65 +0.0 607,354 + nimT MCAODC_18735 0.68 +2.2
Or see this region's nucleotide sequence