Strain Fitness in Escherichia coli ECRC101 around MCAODC_18620

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydjE and tnp-IS629 are separated by 152 nucleotidestnp-IS629 and MCAODC_18625 overlap by 1 nucleotidesMCAODC_18625 and MCAODC_18630 overlap by 26 nucleotides MCAODC_18615: ydjE - Inner membrane metabolite transport protein YdjE, at 582,192 to 583,352 ydjE MCAODC_18620: tnp-IS629 - IS3 family IS629 transposase, at 583,505 to 583,831 tnp-IS629 MCAODC_18625: MCAODC_18625 - IS3 family transposase, at 583,831 to 584,718 _18625 MCAODC_18630: MCAODC_18630 - Insertion sequence 2 OrfA protein, at 584,693 to 584,863 _18630 Position (kb) 583 584Strain fitness (log2 ratio) -1 0 1 2at 582.524 kb on - strand, within ydjEat 582.527 kb on + strand, within ydjEat 582.528 kb on - strand, within ydjEat 582.764 kb on + strand, within ydjEat 582.891 kb on + strand, within ydjEat 582.892 kb on - strand, within ydjEat 583.086 kb on - strand, within ydjEat 583.211 kb on + strand, within ydjEat 583.220 kb on - strand, within ydjEat 583.223 kb on + strand, within ydjEat 583.223 kb on + strand, within ydjEat 583.224 kb on - strand, within ydjEat 583.276 kb on - strandat 583.276 kb on - strandat 583.416 kb on + strandat 584.777 kb on - strand, within MCAODC_18630

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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582,524 - ydjE MCAODC_18615 0.29 +0.2
582,527 + ydjE MCAODC_18615 0.29 -1.4
582,528 - ydjE MCAODC_18615 0.29 +1.2
582,764 + ydjE MCAODC_18615 0.49 +1.6
582,891 + ydjE MCAODC_18615 0.60 +0.1
582,892 - ydjE MCAODC_18615 0.60 +1.3
583,086 - ydjE MCAODC_18615 0.77 -1.5
583,211 + ydjE MCAODC_18615 0.88 +1.6
583,220 - ydjE MCAODC_18615 0.89 -0.7
583,223 + ydjE MCAODC_18615 0.89 +1.3
583,223 + ydjE MCAODC_18615 0.89 +0.6
583,224 - ydjE MCAODC_18615 0.89 +0.5
583,276 - +2.2
583,276 - +0.0
583,416 + -0.4
584,777 - MCAODC_18630 0.49 +1.6

Or see this region's nucleotide sequence