Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cho and ves overlap by 41 nucleotides ves and spy are separated by 202 nucleotides spy and astE are separated by 329 nucleotides
MCAODC_18470: cho - excinuclease Cho, at 552,295 to 553,182
cho
MCAODC_18475: ves - environmental stress-induced protein Ves, at 553,142 to 553,717
ves
MCAODC_18480: spy - ATP-independent periplasmic protein-refolding chaperone Spy, at 553,920 to 554,405
spy
MCAODC_18485: astE - succinylglutamate desuccinylase, at 554,735 to 555,703
astE
Position (kb) 553
554
555 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 553.005 kb on + strand, within cho at 553.065 kb on - strand, within cho at 553.131 kb on + strand at 553.135 kb on + strand at 553.136 kb on - strand at 553.337 kb on - strand, within ves at 553.340 kb on - strand, within ves at 553.711 kb on - strand at 553.747 kb on + strand at 553.792 kb on + strand at 553.792 kb on + strand at 553.943 kb on + strand at 553.992 kb on - strand, within spy at 554.027 kb on - strand, within spy at 554.064 kb on - strand, within spy at 554.064 kb on - strand, within spy at 554.064 kb on - strand, within spy at 554.064 kb on - strand, within spy at 554.124 kb on - strand, within spy at 554.399 kb on + strand at 554.410 kb on + strand at 554.433 kb on - strand at 554.487 kb on + strand at 554.512 kb on + strand at 554.689 kb on + strand at 554.702 kb on + strand at 554.734 kb on - strand at 554.737 kb on - strand at 554.810 kb on + strand at 555.024 kb on - strand, within astE at 555.127 kb on + strand, within astE at 555.224 kb on + strand, within astE at 555.266 kb on + strand, within astE at 555.267 kb on - strand, within astE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 553,005 + cho MCAODC_18470 0.80 -0.4 553,065 - cho MCAODC_18470 0.87 -1.0 553,131 + +1.6 553,135 + +2.2 553,136 - -0.0 553,337 - ves MCAODC_18475 0.34 -1.8 553,340 - ves MCAODC_18475 0.34 -0.4 553,711 - -0.4 553,747 + -0.4 553,792 + -1.0 553,792 + -1.0 553,943 + +1.2 553,992 - spy MCAODC_18480 0.15 +1.0 554,027 - spy MCAODC_18480 0.22 -1.8 554,064 - spy MCAODC_18480 0.30 +1.6 554,064 - spy MCAODC_18480 0.30 -0.5 554,064 - spy MCAODC_18480 0.30 -0.4 554,064 - spy MCAODC_18480 0.30 +0.4 554,124 - spy MCAODC_18480 0.42 +0.5 554,399 + +0.9 554,410 + +1.6 554,433 - -1.5 554,487 + +1.0 554,512 + +1.6 554,689 + -1.8 554,702 + -1.7 554,734 - +1.6 554,737 - +0.8 554,810 + -0.8 555,024 - astE MCAODC_18485 0.30 +0.5 555,127 + astE MCAODC_18485 0.40 +0.6 555,224 + astE MCAODC_18485 0.50 +5.2 555,266 + astE MCAODC_18485 0.55 +2.0 555,267 - astE MCAODC_18485 0.55 -0.1
Or see this region's nucleotide sequence