Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydiV and nlpC are separated by 246 nucleotides nlpC and btuD are separated by 77 nucleotides btuD and btuE overlap by 1 nucleotides btuE and btuC are separated by 62 nucleotides
MCAODC_18290: ydiV - anti-FlhDC factor, at 520,289 to 521,002
ydiV
MCAODC_18295: nlpC - putative endopeptidase NlpC, at 521,249 to 521,713
nlpC
MCAODC_18300: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 521,791 to 522,540
btuD
MCAODC_18305: btuE - bifunctional thioredoxin/glutathione peroxidase, at 522,540 to 523,091
btuE
MCAODC_18310: btuC - vitamin B12 ABC transporter permease BtuC, at 523,154 to 524,134
btuC
Position (kb) 521
522
523 Strain fitness (log2 ratio) -2
-1
0
1
2 at 521.061 kb on - strand at 521.150 kb on - strand at 521.192 kb on - strand at 521.196 kb on + strand at 521.287 kb on - strand at 521.338 kb on + strand, within nlpC at 521.589 kb on - strand, within nlpC at 521.924 kb on - strand, within btuD at 522.224 kb on - strand, within btuD at 522.520 kb on + strand at 522.521 kb on - strand at 522.561 kb on - strand at 522.597 kb on - strand, within btuE at 523.124 kb on - strand at 523.242 kb on - strand at 523.349 kb on - strand, within btuC at 523.494 kb on - strand, within btuC at 523.494 kb on - strand, within btuC at 523.499 kb on - strand, within btuC at 523.499 kb on - strand, within btuC at 523.499 kb on - strand, within btuC
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 521,061 - +0.6 521,150 - +0.0 521,192 - +0.1 521,196 + +1.9 521,287 - -1.2 521,338 + nlpC MCAODC_18295 0.19 +1.4 521,589 - nlpC MCAODC_18295 0.73 -1.0 521,924 - btuD MCAODC_18300 0.18 +0.2 522,224 - btuD MCAODC_18300 0.58 +2.4 522,520 + -1.3 522,521 - -2.1 522,561 - +2.2 522,597 - btuE MCAODC_18305 0.10 +1.6 523,124 - -1.6 523,242 - +0.3 523,349 - btuC MCAODC_18310 0.20 -0.4 523,494 - btuC MCAODC_18310 0.35 -0.4 523,494 - btuC MCAODC_18310 0.35 -1.4 523,499 - btuC MCAODC_18310 0.35 +1.3 523,499 - btuC MCAODC_18310 0.35 +0.9 523,499 - btuC MCAODC_18310 0.35 +0.4
Or see this region's nucleotide sequence