Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_03425 and tyrB are separated by 338 nucleotides tyrB and alr are separated by 252 nucleotides
MCAODC_03425: MCAODC_03425 - hypothetical protein, at 691,905 to 692,264
_03425
MCAODC_03430: tyrB - aromatic amino acid transaminase, at 692,603 to 693,796
tyrB
MCAODC_03435: alr - alanine racemase, at 694,049 to 695,128
alr
Position (kb) 692
693
694 Strain fitness (log2 ratio) -2
-1
0
1
2 at 691.604 kb on - strand at 691.639 kb on + strand at 691.648 kb on + strand at 691.699 kb on + strand at 691.705 kb on + strand at 691.863 kb on - strand at 691.875 kb on - strand at 691.946 kb on - strand, within MCAODC_03425 at 692.010 kb on - strand, within MCAODC_03425 at 692.107 kb on + strand, within MCAODC_03425 at 692.138 kb on + strand, within MCAODC_03425 at 692.142 kb on + strand, within MCAODC_03425 at 692.197 kb on - strand, within MCAODC_03425 at 692.531 kb on - strand at 692.605 kb on - strand at 692.605 kb on - strand at 692.650 kb on + strand at 692.651 kb on - strand at 692.691 kb on + strand at 692.692 kb on - strand at 692.712 kb on - strand at 692.712 kb on - strand at 692.735 kb on + strand, within tyrB at 692.743 kb on + strand, within tyrB at 692.744 kb on - strand, within tyrB at 692.745 kb on + strand, within tyrB at 692.745 kb on + strand, within tyrB at 692.746 kb on - strand, within tyrB at 692.767 kb on + strand, within tyrB at 692.807 kb on + strand, within tyrB at 692.808 kb on - strand, within tyrB at 692.808 kb on - strand, within tyrB at 692.810 kb on + strand, within tyrB at 692.810 kb on + strand, within tyrB at 692.810 kb on + strand, within tyrB at 692.849 kb on - strand, within tyrB at 692.861 kb on + strand, within tyrB at 692.946 kb on + strand, within tyrB at 692.946 kb on + strand, within tyrB at 693.153 kb on + strand, within tyrB at 693.153 kb on + strand, within tyrB at 693.154 kb on - strand, within tyrB at 693.161 kb on - strand, within tyrB at 693.274 kb on + strand, within tyrB at 693.274 kb on + strand, within tyrB at 693.274 kb on + strand, within tyrB at 693.275 kb on - strand, within tyrB at 693.362 kb on + strand, within tyrB at 693.363 kb on - strand, within tyrB at 693.386 kb on + strand, within tyrB at 693.387 kb on - strand, within tyrB at 693.409 kb on + strand, within tyrB at 693.410 kb on - strand, within tyrB at 693.597 kb on - strand, within tyrB at 693.600 kb on + strand, within tyrB at 693.600 kb on + strand, within tyrB at 693.688 kb on - strand at 693.688 kb on - strand at 693.688 kb on - strand at 693.688 kb on - strand at 693.690 kb on + strand at 693.691 kb on - strand at 693.704 kb on + strand at 693.704 kb on + strand at 693.705 kb on - strand at 693.705 kb on - strand at 693.736 kb on + strand at 693.749 kb on - strand at 693.749 kb on - strand at 693.755 kb on - strand at 693.829 kb on + strand at 693.829 kb on + strand at 693.830 kb on - strand at 693.830 kb on - strand at 693.830 kb on - strand at 693.891 kb on - strand at 693.919 kb on + strand at 693.919 kb on + strand at 693.919 kb on + strand at 693.920 kb on - strand at 693.920 kb on - strand at 693.920 kb on - strand at 693.920 kb on - strand at 693.943 kb on + strand at 693.946 kb on - strand at 694.039 kb on + strand at 694.039 kb on + strand at 694.059 kb on + strand at 694.091 kb on - strand at 694.199 kb on + strand, within alr at 694.199 kb on + strand, within alr at 694.200 kb on - strand, within alr at 694.205 kb on + strand, within alr at 694.216 kb on + strand, within alr at 694.323 kb on - strand, within alr at 694.387 kb on + strand, within alr at 694.387 kb on + strand, within alr at 694.388 kb on - strand, within alr at 694.591 kb on + strand, within alr at 694.594 kb on + strand, within alr at 694.707 kb on + strand, within alr at 694.751 kb on - strand, within alr
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 691,604 - +0.9 691,639 + -1.0 691,648 + -0.2 691,699 + +0.6 691,705 + -0.3 691,863 - +0.9 691,875 - -0.1 691,946 - MCAODC_03425 0.11 -0.3 692,010 - MCAODC_03425 0.29 +1.1 692,107 + MCAODC_03425 0.56 +0.9 692,138 + MCAODC_03425 0.65 +0.9 692,142 + MCAODC_03425 0.66 +0.8 692,197 - MCAODC_03425 0.81 +2.4 692,531 - -0.5 692,605 - -1.6 692,605 - +1.6 692,650 + +0.5 692,651 - +1.5 692,691 + -2.4 692,692 - +0.1 692,712 - -1.4 692,712 - -0.2 692,735 + tyrB MCAODC_03430 0.11 -0.3 692,743 + tyrB MCAODC_03430 0.12 +0.8 692,744 - tyrB MCAODC_03430 0.12 +0.4 692,745 + tyrB MCAODC_03430 0.12 -0.7 692,745 + tyrB MCAODC_03430 0.12 -0.5 692,746 - tyrB MCAODC_03430 0.12 -1.8 692,767 + tyrB MCAODC_03430 0.14 +1.9 692,807 + tyrB MCAODC_03430 0.17 +2.5 692,808 - tyrB MCAODC_03430 0.17 +0.3 692,808 - tyrB MCAODC_03430 0.17 -0.0 692,810 + tyrB MCAODC_03430 0.17 -0.4 692,810 + tyrB MCAODC_03430 0.17 +0.8 692,810 + tyrB MCAODC_03430 0.17 +0.0 692,849 - tyrB MCAODC_03430 0.21 -1.4 692,861 + tyrB MCAODC_03430 0.22 -0.1 692,946 + tyrB MCAODC_03430 0.29 -0.6 692,946 + tyrB MCAODC_03430 0.29 -2.1 693,153 + tyrB MCAODC_03430 0.46 +0.1 693,153 + tyrB MCAODC_03430 0.46 -1.1 693,154 - tyrB MCAODC_03430 0.46 -2.2 693,161 - tyrB MCAODC_03430 0.47 -1.6 693,274 + tyrB MCAODC_03430 0.56 +1.9 693,274 + tyrB MCAODC_03430 0.56 +0.9 693,274 + tyrB MCAODC_03430 0.56 -0.0 693,275 - tyrB MCAODC_03430 0.56 +1.1 693,362 + tyrB MCAODC_03430 0.64 +0.9 693,363 - tyrB MCAODC_03430 0.64 -0.4 693,386 + tyrB MCAODC_03430 0.66 +0.4 693,387 - tyrB MCAODC_03430 0.66 -0.4 693,409 + tyrB MCAODC_03430 0.68 -0.3 693,410 - tyrB MCAODC_03430 0.68 +0.2 693,597 - tyrB MCAODC_03430 0.83 +1.9 693,600 + tyrB MCAODC_03430 0.84 +0.8 693,600 + tyrB MCAODC_03430 0.84 -2.2 693,688 - -0.1 693,688 - -1.3 693,688 - -1.0 693,688 - +0.4 693,690 + -0.3 693,691 - -0.2 693,704 + +0.1 693,704 + -2.6 693,705 - +0.9 693,705 - +0.2 693,736 + +0.1 693,749 - -0.9 693,749 - +0.7 693,755 - +1.2 693,829 + +0.9 693,829 + -0.6 693,830 - +1.1 693,830 - +1.5 693,830 - -0.2 693,891 - -0.2 693,919 + +0.0 693,919 + +0.4 693,919 + -0.1 693,920 - -0.2 693,920 - +0.4 693,920 - -1.9 693,920 - -1.2 693,943 + +0.6 693,946 - -0.3 694,039 + +1.4 694,039 + +2.2 694,059 + -0.2 694,091 - -1.2 694,199 + alr MCAODC_03435 0.14 -1.4 694,199 + alr MCAODC_03435 0.14 -0.6 694,200 - alr MCAODC_03435 0.14 -1.8 694,205 + alr MCAODC_03435 0.14 +1.9 694,216 + alr MCAODC_03435 0.15 -0.5 694,323 - alr MCAODC_03435 0.25 -1.7 694,387 + alr MCAODC_03435 0.31 -1.5 694,387 + alr MCAODC_03435 0.31 -1.1 694,388 - alr MCAODC_03435 0.31 -0.4 694,591 + alr MCAODC_03435 0.50 +0.9 694,594 + alr MCAODC_03435 0.50 -0.1 694,707 + alr MCAODC_03435 0.61 -0.9 694,751 - alr MCAODC_03435 0.65 +0.9
Or see this region's nucleotide sequence