Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysS and ghoT are separated by 118 nucleotides ghoT and ghoS are separated by 27 nucleotides ghoS and yjdJ are separated by 226 nucleotides yjdJ and yjdI are separated by 11 nucleotides yjdI and dcuS are separated by 180 nucleotides
MCAODC_02995: lysS - lysine--tRNA ligase, at 604,898 to 606,415
lysS
MCAODC_03000: ghoT - type V toxin-antitoxin system toxin GhoT, at 606,534 to 606,707
ghoT
MCAODC_03005: ghoS - type V toxin-antitoxin system endoribonuclease antitoxin GhoS, at 606,735 to 607,031
ghoS
MCAODC_03010: yjdJ - Uncharacterized protein YjdJ, at 607,258 to 607,530
yjdJ
MCAODC_03015: yjdI - Uncharacterized protein YjdI, at 607,542 to 607,772
yjdI
MCAODC_03020: dcuS - Sensor histidine kinase DcuS, at 607,953 to 609,584
dcuS
Position (kb) 607
608 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 606.266 kb on + strand at 606.267 kb on - strand at 606.278 kb on + strand at 606.296 kb on + strand at 606.297 kb on - strand at 606.297 kb on - strand at 606.346 kb on + strand at 606.346 kb on + strand at 606.346 kb on + strand at 606.347 kb on - strand at 606.356 kb on - strand at 606.358 kb on + strand at 606.367 kb on + strand at 606.368 kb on - strand at 606.370 kb on + strand at 606.371 kb on - strand at 606.382 kb on + strand at 606.382 kb on + strand at 606.413 kb on + strand at 606.413 kb on + strand at 606.527 kb on - strand at 606.527 kb on - strand at 606.583 kb on - strand, within ghoT at 606.602 kb on + strand, within ghoT at 606.603 kb on - strand, within ghoT at 606.603 kb on - strand, within ghoT at 606.603 kb on - strand, within ghoT at 606.615 kb on - strand, within ghoT at 606.712 kb on + strand at 606.736 kb on + strand at 606.758 kb on - strand at 606.774 kb on - strand, within ghoS at 606.807 kb on + strand, within ghoS at 606.816 kb on - strand, within ghoS at 606.894 kb on + strand, within ghoS at 606.895 kb on - strand, within ghoS at 606.918 kb on - strand, within ghoS at 606.937 kb on + strand, within ghoS at 606.938 kb on - strand, within ghoS at 607.003 kb on + strand at 607.004 kb on - strand at 607.004 kb on - strand at 607.004 kb on - strand at 607.006 kb on - strand at 607.008 kb on - strand at 607.101 kb on + strand at 607.101 kb on + strand at 607.104 kb on - strand at 607.130 kb on - strand at 607.278 kb on - strand at 607.278 kb on - strand at 607.278 kb on - strand at 607.420 kb on - strand, within yjdJ at 607.495 kb on - strand, within yjdJ at 607.533 kb on + strand at 607.555 kb on + strand at 607.580 kb on + strand, within yjdI at 607.649 kb on + strand, within yjdI at 607.650 kb on - strand, within yjdI at 607.653 kb on + strand, within yjdI at 607.671 kb on + strand, within yjdI at 607.671 kb on + strand, within yjdI at 607.672 kb on - strand, within yjdI at 607.696 kb on - strand, within yjdI at 607.696 kb on - strand, within yjdI at 607.696 kb on - strand, within yjdI at 607.735 kb on + strand, within yjdI at 607.735 kb on + strand, within yjdI at 607.736 kb on - strand, within yjdI at 607.794 kb on - strand at 607.805 kb on + strand at 607.805 kb on + strand at 607.805 kb on + strand at 607.805 kb on + strand at 607.807 kb on + strand at 607.807 kb on + strand at 607.807 kb on + strand at 607.807 kb on + strand at 607.808 kb on - strand at 607.808 kb on - strand at 607.808 kb on - strand at 607.831 kb on - strand at 607.869 kb on + strand at 607.870 kb on - strand at 607.870 kb on - strand at 607.871 kb on + strand at 607.871 kb on + strand at 607.871 kb on + strand at 607.871 kb on + strand at 607.872 kb on - strand at 607.872 kb on - strand at 607.872 kb on - strand at 607.873 kb on + strand at 607.873 kb on + strand at 607.873 kb on + strand at 607.874 kb on - strand at 607.874 kb on - strand at 607.887 kb on + strand at 607.887 kb on + strand at 607.888 kb on - strand at 607.919 kb on + strand at 607.919 kb on + strand at 607.981 kb on + strand at 607.981 kb on + strand at 608.020 kb on + strand at 608.020 kb on + strand at 608.020 kb on + strand at 608.021 kb on - strand at 608.021 kb on - strand at 608.021 kb on - strand at 608.071 kb on - strand at 608.090 kb on + strand at 608.091 kb on - strand at 608.243 kb on + strand, within dcuS at 608.243 kb on + strand, within dcuS at 608.275 kb on - strand, within dcuS at 608.298 kb on - strand, within dcuS at 608.313 kb on - strand, within dcuS at 608.331 kb on - strand, within dcuS at 608.395 kb on + strand, within dcuS at 608.395 kb on + strand, within dcuS at 608.396 kb on - strand, within dcuS at 608.409 kb on + strand, within dcuS at 608.409 kb on + strand, within dcuS at 608.410 kb on - strand, within dcuS at 608.458 kb on + strand, within dcuS at 608.501 kb on + strand, within dcuS at 608.501 kb on + strand, within dcuS at 608.501 kb on + strand, within dcuS at 608.502 kb on - strand, within dcuS at 608.515 kb on + strand, within dcuS at 608.515 kb on + strand, within dcuS at 608.516 kb on - strand, within dcuS at 608.516 kb on - strand, within dcuS
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 606,266 + -0.1 606,267 - -0.1 606,278 + -0.7 606,296 + -1.3 606,297 - +2.2 606,297 - +0.7 606,346 + +0.4 606,346 + -0.3 606,346 + -0.7 606,347 - +0.1 606,356 - +1.1 606,358 + +1.1 606,367 + +1.1 606,368 - -1.4 606,370 + +0.0 606,371 - +0.8 606,382 + -0.3 606,382 + -0.1 606,413 + +0.2 606,413 + -1.1 606,527 - +0.1 606,527 - +0.5 606,583 - ghoT MCAODC_03000 0.28 +0.5 606,602 + ghoT MCAODC_03000 0.39 +0.6 606,603 - ghoT MCAODC_03000 0.40 -0.9 606,603 - ghoT MCAODC_03000 0.40 -0.9 606,603 - ghoT MCAODC_03000 0.40 +0.3 606,615 - ghoT MCAODC_03000 0.47 -0.5 606,712 + +0.5 606,736 + +1.9 606,758 - +1.4 606,774 - ghoS MCAODC_03005 0.13 -1.4 606,807 + ghoS MCAODC_03005 0.24 +1.8 606,816 - ghoS MCAODC_03005 0.27 +0.1 606,894 + ghoS MCAODC_03005 0.54 +0.6 606,895 - ghoS MCAODC_03005 0.54 -0.9 606,918 - ghoS MCAODC_03005 0.62 -0.1 606,937 + ghoS MCAODC_03005 0.68 -0.2 606,938 - ghoS MCAODC_03005 0.68 -1.5 607,003 + +0.5 607,004 - -1.2 607,004 - +0.9 607,004 - +0.1 607,006 - +0.6 607,008 - +0.4 607,101 + -2.8 607,101 + -0.1 607,104 - -0.9 607,130 - -0.4 607,278 - +1.9 607,278 - -1.8 607,278 - +0.8 607,420 - yjdJ MCAODC_03010 0.59 -0.6 607,495 - yjdJ MCAODC_03010 0.87 -0.9 607,533 + +0.7 607,555 + +0.3 607,580 + yjdI MCAODC_03015 0.16 -0.4 607,649 + yjdI MCAODC_03015 0.46 +0.7 607,650 - yjdI MCAODC_03015 0.47 -0.2 607,653 + yjdI MCAODC_03015 0.48 +0.8 607,671 + yjdI MCAODC_03015 0.56 +1.9 607,671 + yjdI MCAODC_03015 0.56 -1.2 607,672 - yjdI MCAODC_03015 0.56 -0.6 607,696 - yjdI MCAODC_03015 0.67 +1.5 607,696 - yjdI MCAODC_03015 0.67 -1.9 607,696 - yjdI MCAODC_03015 0.67 +0.3 607,735 + yjdI MCAODC_03015 0.84 +1.7 607,735 + yjdI MCAODC_03015 0.84 +0.0 607,736 - yjdI MCAODC_03015 0.84 -0.5 607,794 - -1.5 607,805 + -1.3 607,805 + -1.9 607,805 + -0.1 607,805 + +0.7 607,807 + +0.7 607,807 + +0.1 607,807 + -1.3 607,807 + -1.2 607,808 - -0.3 607,808 - -0.3 607,808 - +0.7 607,831 - +2.7 607,869 + -0.2 607,870 - -0.7 607,870 - -0.8 607,871 + +1.9 607,871 + +0.3 607,871 + +0.9 607,871 + +0.6 607,872 - +0.3 607,872 - -0.3 607,872 - -0.1 607,873 + +0.3 607,873 + +0.1 607,873 + +1.8 607,874 - +2.1 607,874 - -0.6 607,887 + -1.3 607,887 + -0.4 607,888 - +0.3 607,919 + -0.2 607,919 + +3.5 607,981 + -0.9 607,981 + -1.2 608,020 + +0.5 608,020 + -0.8 608,020 + -1.3 608,021 - -0.3 608,021 - -2.0 608,021 - +0.3 608,071 - +1.3 608,090 + -0.4 608,091 - +0.5 608,243 + dcuS MCAODC_03020 0.18 -0.3 608,243 + dcuS MCAODC_03020 0.18 +0.3 608,275 - dcuS MCAODC_03020 0.20 -0.8 608,298 - dcuS MCAODC_03020 0.21 -0.6 608,313 - dcuS MCAODC_03020 0.22 -0.9 608,331 - dcuS MCAODC_03020 0.23 +0.5 608,395 + dcuS MCAODC_03020 0.27 -0.2 608,395 + dcuS MCAODC_03020 0.27 +0.5 608,396 - dcuS MCAODC_03020 0.27 -1.2 608,409 + dcuS MCAODC_03020 0.28 -0.7 608,409 + dcuS MCAODC_03020 0.28 -0.6 608,410 - dcuS MCAODC_03020 0.28 +0.2 608,458 + dcuS MCAODC_03020 0.31 -0.7 608,501 + dcuS MCAODC_03020 0.34 +0.3 608,501 + dcuS MCAODC_03020 0.34 -1.6 608,501 + dcuS MCAODC_03020 0.34 -1.1 608,502 - dcuS MCAODC_03020 0.34 -1.2 608,515 + dcuS MCAODC_03020 0.34 -1.8 608,515 + dcuS MCAODC_03020 0.34 -1.8 608,516 - dcuS MCAODC_03020 0.34 -0.9 608,516 - dcuS MCAODC_03020 0.34 +0.1
Or see this region's nucleotide sequence