Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fklB and ytfB are separated by 217 nucleotides ytfB and insQ are separated by 134 nucleotides
MCAODC_02590: fklB - FKBP-type peptidyl-prolyl cis-trans isomerase, at 529,036 to 529,656
fklB
MCAODC_02595: ytfB - cell division protein YtfB, at 529,874 to 530,512
ytfB
MCAODC_02600: insQ - Transposase, at 530,647 to 531,795
insQ
Position (kb) 529
530
531 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 528.874 kb on - strand at 528.879 kb on + strand at 528.880 kb on - strand at 528.936 kb on - strand at 529.037 kb on + strand at 529.116 kb on - strand, within fklB at 529.134 kb on + strand, within fklB at 529.135 kb on - strand, within fklB at 529.135 kb on - strand, within fklB at 529.157 kb on - strand, within fklB at 529.269 kb on + strand, within fklB at 529.277 kb on + strand, within fklB at 529.278 kb on - strand, within fklB at 529.278 kb on - strand, within fklB at 529.278 kb on - strand, within fklB at 529.278 kb on - strand, within fklB at 529.300 kb on - strand, within fklB at 529.300 kb on - strand, within fklB at 529.363 kb on - strand, within fklB at 529.363 kb on - strand, within fklB at 529.533 kb on + strand, within fklB at 529.533 kb on + strand, within fklB at 529.659 kb on + strand at 529.676 kb on + strand at 529.676 kb on + strand at 529.677 kb on - strand at 529.677 kb on - strand at 529.678 kb on + strand at 529.678 kb on + strand at 529.679 kb on - strand at 529.679 kb on - strand at 529.683 kb on + strand at 529.696 kb on - strand at 529.766 kb on - strand at 529.766 kb on - strand at 529.784 kb on + strand at 529.784 kb on + strand at 529.784 kb on + strand at 529.784 kb on + strand at 529.784 kb on + strand at 529.785 kb on - strand at 529.786 kb on + strand at 529.786 kb on + strand at 529.786 kb on + strand at 529.786 kb on + strand at 529.787 kb on - strand at 529.787 kb on - strand at 529.790 kb on - strand at 529.790 kb on - strand at 529.815 kb on + strand at 529.821 kb on + strand at 529.822 kb on - strand at 529.822 kb on - strand at 529.826 kb on + strand at 529.827 kb on - strand at 529.827 kb on - strand at 529.832 kb on + strand at 529.832 kb on + strand at 529.838 kb on - strand at 529.894 kb on - strand at 529.981 kb on + strand, within ytfB at 529.981 kb on + strand, within ytfB at 529.995 kb on + strand, within ytfB at 529.995 kb on + strand, within ytfB at 529.995 kb on + strand, within ytfB at 529.996 kb on - strand, within ytfB at 530.029 kb on + strand, within ytfB at 530.030 kb on - strand, within ytfB at 530.067 kb on + strand, within ytfB at 530.067 kb on + strand, within ytfB at 530.068 kb on - strand, within ytfB at 530.121 kb on + strand, within ytfB at 530.122 kb on - strand, within ytfB at 530.138 kb on + strand, within ytfB at 530.174 kb on - strand, within ytfB at 530.174 kb on - strand, within ytfB at 530.174 kb on - strand, within ytfB at 530.175 kb on + strand, within ytfB at 530.175 kb on + strand, within ytfB at 530.248 kb on + strand, within ytfB at 530.249 kb on - strand, within ytfB at 530.334 kb on - strand, within ytfB at 530.334 kb on - strand, within ytfB at 530.334 kb on - strand, within ytfB at 530.334 kb on - strand, within ytfB at 530.349 kb on + strand, within ytfB at 530.349 kb on + strand, within ytfB at 530.349 kb on + strand, within ytfB at 530.349 kb on + strand, within ytfB at 530.349 kb on + strand, within ytfB at 530.456 kb on - strand at 530.498 kb on - strand at 530.510 kb on + strand at 530.510 kb on + strand at 530.561 kb on + strand at 530.561 kb on + strand at 530.561 kb on + strand at 530.561 kb on + strand at 530.562 kb on - strand at 530.562 kb on - strand at 530.562 kb on - strand at 530.562 kb on - strand at 530.562 kb on - strand at 530.562 kb on - strand at 530.562 kb on - strand at 530.562 kb on - strand at 530.594 kb on + strand at 530.595 kb on - strand at 530.764 kb on + strand, within insQ at 530.782 kb on - strand, within insQ at 530.782 kb on - strand, within insQ at 530.782 kb on - strand, within insQ at 530.782 kb on - strand, within insQ at 530.782 kb on - strand, within insQ at 530.808 kb on + strand, within insQ at 530.870 kb on + strand, within insQ at 530.870 kb on + strand, within insQ at 530.919 kb on - strand, within insQ at 530.919 kb on - strand, within insQ at 531.202 kb on + strand, within insQ at 531.378 kb on - strand, within insQ at 531.378 kb on - strand, within insQ at 531.378 kb on - strand, within insQ at 531.378 kb on - strand, within insQ at 531.378 kb on - strand, within insQ at 531.438 kb on + strand, within insQ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 528,874 - +1.1 528,879 + +0.0 528,880 - -0.2 528,936 - +0.0 529,037 + -0.2 529,116 - fklB MCAODC_02590 0.13 -0.5 529,134 + fklB MCAODC_02590 0.16 -1.8 529,135 - fklB MCAODC_02590 0.16 +1.2 529,135 - fklB MCAODC_02590 0.16 -0.8 529,157 - fklB MCAODC_02590 0.19 -0.6 529,269 + fklB MCAODC_02590 0.38 -2.0 529,277 + fklB MCAODC_02590 0.39 +0.4 529,278 - fklB MCAODC_02590 0.39 -1.4 529,278 - fklB MCAODC_02590 0.39 -1.2 529,278 - fklB MCAODC_02590 0.39 +0.6 529,278 - fklB MCAODC_02590 0.39 -1.1 529,300 - fklB MCAODC_02590 0.43 +0.7 529,300 - fklB MCAODC_02590 0.43 +0.7 529,363 - fklB MCAODC_02590 0.53 +1.5 529,363 - fklB MCAODC_02590 0.53 +0.3 529,533 + fklB MCAODC_02590 0.80 +0.5 529,533 + fklB MCAODC_02590 0.80 +0.0 529,659 + +0.8 529,676 + +0.1 529,676 + -0.0 529,677 - +0.0 529,677 - -0.9 529,678 + -2.7 529,678 + +0.9 529,679 - -2.5 529,679 - -0.1 529,683 + +0.1 529,696 - -0.4 529,766 - -0.9 529,766 - -0.7 529,784 + +1.7 529,784 + -1.9 529,784 + -1.1 529,784 + -0.2 529,784 + +0.1 529,785 - -0.4 529,786 + -0.8 529,786 + -1.4 529,786 + -2.1 529,786 + -0.1 529,787 - +0.6 529,787 - +0.2 529,790 - +0.1 529,790 - +0.8 529,815 + +0.3 529,821 + +0.0 529,822 - +1.9 529,822 - -0.2 529,826 + -1.6 529,827 - -0.3 529,827 - -0.2 529,832 + +3.2 529,832 + -0.7 529,838 - -0.7 529,894 - -0.2 529,981 + ytfB MCAODC_02595 0.17 -0.2 529,981 + ytfB MCAODC_02595 0.17 +0.6 529,995 + ytfB MCAODC_02595 0.19 +0.8 529,995 + ytfB MCAODC_02595 0.19 +0.7 529,995 + ytfB MCAODC_02595 0.19 -1.6 529,996 - ytfB MCAODC_02595 0.19 -0.8 530,029 + ytfB MCAODC_02595 0.24 +0.1 530,030 - ytfB MCAODC_02595 0.24 +0.8 530,067 + ytfB MCAODC_02595 0.30 -1.1 530,067 + ytfB MCAODC_02595 0.30 +1.5 530,068 - ytfB MCAODC_02595 0.30 -2.2 530,121 + ytfB MCAODC_02595 0.39 +0.5 530,122 - ytfB MCAODC_02595 0.39 -0.5 530,138 + ytfB MCAODC_02595 0.41 +0.9 530,174 - ytfB MCAODC_02595 0.47 -0.7 530,174 - ytfB MCAODC_02595 0.47 -0.6 530,174 - ytfB MCAODC_02595 0.47 -1.1 530,175 + ytfB MCAODC_02595 0.47 +0.2 530,175 + ytfB MCAODC_02595 0.47 -0.5 530,248 + ytfB MCAODC_02595 0.59 +0.7 530,249 - ytfB MCAODC_02595 0.59 +0.5 530,334 - ytfB MCAODC_02595 0.72 +0.9 530,334 - ytfB MCAODC_02595 0.72 -0.8 530,334 - ytfB MCAODC_02595 0.72 -0.8 530,334 - ytfB MCAODC_02595 0.72 -0.3 530,349 + ytfB MCAODC_02595 0.74 -0.9 530,349 + ytfB MCAODC_02595 0.74 -0.9 530,349 + ytfB MCAODC_02595 0.74 -0.8 530,349 + ytfB MCAODC_02595 0.74 -0.2 530,349 + ytfB MCAODC_02595 0.74 -0.7 530,456 - +1.0 530,498 - -0.9 530,510 + +2.9 530,510 + +0.9 530,561 + +0.1 530,561 + -0.7 530,561 + -0.8 530,561 + +0.3 530,562 - +0.3 530,562 - -0.1 530,562 - -0.1 530,562 - +0.8 530,562 - -0.5 530,562 - +0.9 530,562 - +0.4 530,562 - +0.2 530,594 + -0.2 530,595 - -0.4 530,764 + insQ MCAODC_02600 0.10 +1.3 530,782 - insQ MCAODC_02600 0.12 +1.3 530,782 - insQ MCAODC_02600 0.12 -0.4 530,782 - insQ MCAODC_02600 0.12 +0.0 530,782 - insQ MCAODC_02600 0.12 -0.3 530,782 - insQ MCAODC_02600 0.12 -0.1 530,808 + insQ MCAODC_02600 0.14 -1.3 530,870 + insQ MCAODC_02600 0.19 +0.7 530,870 + insQ MCAODC_02600 0.19 -0.9 530,919 - insQ MCAODC_02600 0.24 +0.2 530,919 - insQ MCAODC_02600 0.24 +0.6 531,202 + insQ MCAODC_02600 0.48 -0.4 531,378 - insQ MCAODC_02600 0.64 -0.0 531,378 - insQ MCAODC_02600 0.64 -1.1 531,378 - insQ MCAODC_02600 0.64 +0.8 531,378 - insQ MCAODC_02600 0.64 -1.1 531,378 - insQ MCAODC_02600 0.64 -1.0 531,438 + insQ MCAODC_02600 0.69 +2.1
Or see this region's nucleotide sequence