Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nlpE and arfB are separated by 13 nucleotides arfB and yaeQ overlap by 4 nucleotides yaeQ and yaeP are separated by 165 nucleotides yaeP and rof overlap by 14 nucleotides rof and tilS are separated by 52 nucleotides
MCAODC_00605: nlpE - envelope stress response activation lipoprotein NlpE, at 97,899 to 98,609
nlpE
MCAODC_00610: arfB - aminoacyl-tRNA hydrolase, at 98,623 to 99,033
arfB
MCAODC_00615: yaeQ - Uncharacterized protein YaeQ, at 99,030 to 99,575
yaeQ
MCAODC_00620: yaeP - YaeP family protein, at 99,741 to 99,941
yaeP
MCAODC_00625: rof - Rho-binding antiterminator, at 99,928 to 100,188
rof
MCAODC_00630: tilS - tRNA lysidine(34) synthetase TilS, at 100,241 to 101,536
tilS
Position (kb) 99
100 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 98.146 kb on + strand, within nlpE at 98.152 kb on + strand, within nlpE at 98.152 kb on + strand, within nlpE at 98.185 kb on - strand, within nlpE at 98.211 kb on - strand, within nlpE at 98.212 kb on + strand, within nlpE at 98.213 kb on - strand, within nlpE at 98.213 kb on - strand, within nlpE at 98.213 kb on - strand, within nlpE at 98.213 kb on - strand, within nlpE at 98.281 kb on + strand, within nlpE at 98.282 kb on - strand, within nlpE at 98.284 kb on + strand, within nlpE at 98.284 kb on + strand, within nlpE at 98.313 kb on + strand, within nlpE at 98.342 kb on + strand, within nlpE at 98.342 kb on + strand, within nlpE at 98.343 kb on - strand, within nlpE at 98.343 kb on - strand, within nlpE at 98.347 kb on + strand, within nlpE at 98.526 kb on - strand, within nlpE at 98.526 kb on - strand, within nlpE at 98.544 kb on - strand at 98.547 kb on - strand at 98.574 kb on + strand at 98.575 kb on - strand at 98.740 kb on + strand, within arfB at 98.741 kb on - strand, within arfB at 98.741 kb on - strand, within arfB at 98.749 kb on - strand, within arfB at 98.818 kb on - strand, within arfB at 98.827 kb on + strand, within arfB at 98.827 kb on + strand, within arfB at 98.879 kb on + strand, within arfB at 98.882 kb on + strand, within arfB at 98.940 kb on + strand, within arfB at 98.943 kb on + strand, within arfB at 98.943 kb on + strand, within arfB at 98.944 kb on - strand, within arfB at 98.944 kb on - strand, within arfB at 98.944 kb on - strand, within arfB at 99.006 kb on + strand at 99.072 kb on - strand at 99.077 kb on + strand at 99.078 kb on - strand at 99.132 kb on - strand, within yaeQ at 99.132 kb on - strand, within yaeQ at 99.178 kb on + strand, within yaeQ at 99.179 kb on - strand, within yaeQ at 99.251 kb on + strand, within yaeQ at 99.251 kb on + strand, within yaeQ at 99.252 kb on - strand, within yaeQ at 99.254 kb on + strand, within yaeQ at 99.254 kb on + strand, within yaeQ at 99.255 kb on - strand, within yaeQ at 99.256 kb on + strand, within yaeQ at 99.256 kb on + strand, within yaeQ at 99.256 kb on + strand, within yaeQ at 99.257 kb on - strand, within yaeQ at 99.257 kb on - strand, within yaeQ at 99.257 kb on - strand, within yaeQ at 99.257 kb on - strand, within yaeQ at 99.260 kb on + strand, within yaeQ at 99.260 kb on + strand, within yaeQ at 99.261 kb on - strand, within yaeQ at 99.261 kb on - strand, within yaeQ at 99.427 kb on + strand, within yaeQ at 99.427 kb on + strand, within yaeQ at 99.428 kb on - strand, within yaeQ at 99.428 kb on - strand, within yaeQ at 99.458 kb on + strand, within yaeQ at 99.458 kb on + strand, within yaeQ at 99.459 kb on - strand, within yaeQ at 99.540 kb on - strand at 99.551 kb on + strand at 99.554 kb on - strand at 99.554 kb on - strand at 99.575 kb on + strand at 99.616 kb on + strand at 99.616 kb on + strand at 99.617 kb on - strand at 99.617 kb on - strand at 99.637 kb on + strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.657 kb on + strand at 99.657 kb on + strand at 99.658 kb on - strand at 99.658 kb on - strand at 99.658 kb on - strand at 99.659 kb on + strand at 99.659 kb on + strand at 99.683 kb on - strand at 99.691 kb on - strand at 99.696 kb on + strand at 99.696 kb on + strand at 99.697 kb on - strand at 99.763 kb on + strand, within yaeP at 99.799 kb on + strand, within yaeP at 99.799 kb on + strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.804 kb on + strand, within yaeP at 99.804 kb on + strand, within yaeP at 99.804 kb on + strand, within yaeP at 99.805 kb on - strand, within yaeP at 99.897 kb on + strand, within yaeP at 99.897 kb on + strand, within yaeP at 99.897 kb on + strand, within yaeP at 99.928 kb on - strand at 99.946 kb on + strand at 99.946 kb on + strand at 99.946 kb on + strand at 99.946 kb on + strand at 99.946 kb on + strand at 99.947 kb on - strand at 99.947 kb on - strand at 99.970 kb on + strand, within rof at 99.970 kb on + strand, within rof at 99.970 kb on + strand, within rof at 99.971 kb on - strand, within rof at 100.004 kb on + strand, within rof at 100.005 kb on - strand, within rof at 100.005 kb on - strand, within rof at 100.005 kb on - strand, within rof at 100.005 kb on - strand, within rof at 100.121 kb on + strand, within rof at 100.122 kb on - strand, within rof at 100.146 kb on + strand, within rof at 100.213 kb on + strand at 100.224 kb on - strand at 100.242 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 98,146 + nlpE MCAODC_00605 0.35 +0.9 98,152 + nlpE MCAODC_00605 0.36 -1.1 98,152 + nlpE MCAODC_00605 0.36 +1.4 98,185 - nlpE MCAODC_00605 0.40 +0.0 98,211 - nlpE MCAODC_00605 0.44 -0.0 98,212 + nlpE MCAODC_00605 0.44 -0.7 98,213 - nlpE MCAODC_00605 0.44 -0.9 98,213 - nlpE MCAODC_00605 0.44 +0.3 98,213 - nlpE MCAODC_00605 0.44 -0.7 98,213 - nlpE MCAODC_00605 0.44 +1.2 98,281 + nlpE MCAODC_00605 0.54 +0.9 98,282 - nlpE MCAODC_00605 0.54 +0.4 98,284 + nlpE MCAODC_00605 0.54 +0.3 98,284 + nlpE MCAODC_00605 0.54 -0.6 98,313 + nlpE MCAODC_00605 0.58 +1.7 98,342 + nlpE MCAODC_00605 0.62 +1.7 98,342 + nlpE MCAODC_00605 0.62 +0.1 98,343 - nlpE MCAODC_00605 0.62 +0.9 98,343 - nlpE MCAODC_00605 0.62 -1.4 98,347 + nlpE MCAODC_00605 0.63 +1.3 98,526 - nlpE MCAODC_00605 0.88 -0.9 98,526 - nlpE MCAODC_00605 0.88 +1.5 98,544 - +0.7 98,547 - -0.3 98,574 + +1.9 98,575 - -0.2 98,740 + arfB MCAODC_00610 0.28 -0.9 98,741 - arfB MCAODC_00610 0.29 -0.1 98,741 - arfB MCAODC_00610 0.29 +0.5 98,749 - arfB MCAODC_00610 0.31 -0.9 98,818 - arfB MCAODC_00610 0.47 -0.4 98,827 + arfB MCAODC_00610 0.50 +1.3 98,827 + arfB MCAODC_00610 0.50 -0.3 98,879 + arfB MCAODC_00610 0.62 +0.9 98,882 + arfB MCAODC_00610 0.63 -1.2 98,940 + arfB MCAODC_00610 0.77 +0.3 98,943 + arfB MCAODC_00610 0.78 -2.3 98,943 + arfB MCAODC_00610 0.78 -0.5 98,944 - arfB MCAODC_00610 0.78 -0.9 98,944 - arfB MCAODC_00610 0.78 +0.2 98,944 - arfB MCAODC_00610 0.78 +0.1 99,006 + +0.1 99,072 - +1.6 99,077 + +0.5 99,078 - +0.7 99,132 - yaeQ MCAODC_00615 0.19 -0.5 99,132 - yaeQ MCAODC_00615 0.19 +0.7 99,178 + yaeQ MCAODC_00615 0.27 +0.1 99,179 - yaeQ MCAODC_00615 0.27 -0.7 99,251 + yaeQ MCAODC_00615 0.40 -1.9 99,251 + yaeQ MCAODC_00615 0.40 -1.2 99,252 - yaeQ MCAODC_00615 0.41 +0.6 99,254 + yaeQ MCAODC_00615 0.41 -0.1 99,254 + yaeQ MCAODC_00615 0.41 +1.0 99,255 - yaeQ MCAODC_00615 0.41 -3.0 99,256 + yaeQ MCAODC_00615 0.41 -0.9 99,256 + yaeQ MCAODC_00615 0.41 +3.1 99,256 + yaeQ MCAODC_00615 0.41 +1.9 99,257 - yaeQ MCAODC_00615 0.42 +0.6 99,257 - yaeQ MCAODC_00615 0.42 -0.5 99,257 - yaeQ MCAODC_00615 0.42 +0.9 99,257 - yaeQ MCAODC_00615 0.42 +0.3 99,260 + yaeQ MCAODC_00615 0.42 +0.1 99,260 + yaeQ MCAODC_00615 0.42 -2.5 99,261 - yaeQ MCAODC_00615 0.42 -1.4 99,261 - yaeQ MCAODC_00615 0.42 -0.0 99,427 + yaeQ MCAODC_00615 0.73 +0.2 99,427 + yaeQ MCAODC_00615 0.73 +0.7 99,428 - yaeQ MCAODC_00615 0.73 +0.7 99,428 - yaeQ MCAODC_00615 0.73 -0.2 99,458 + yaeQ MCAODC_00615 0.78 +0.8 99,458 + yaeQ MCAODC_00615 0.78 -0.7 99,459 - yaeQ MCAODC_00615 0.79 -0.8 99,540 - +1.3 99,551 + -0.3 99,554 - +0.9 99,554 - +0.9 99,575 + -1.9 99,616 + +1.2 99,616 + +1.5 99,617 - -1.0 99,617 - +2.9 99,637 + +0.6 99,638 - -0.8 99,638 - -0.5 99,657 + +0.8 99,657 + +0.6 99,658 - -0.8 99,658 - -1.4 99,658 - -1.1 99,659 + -1.2 99,659 + +0.9 99,683 - -2.1 99,691 - -0.5 99,696 + +1.9 99,696 + +0.8 99,697 - -1.6 99,763 + yaeP MCAODC_00620 0.11 +0.2 99,799 + yaeP MCAODC_00620 0.29 -0.3 99,799 + yaeP MCAODC_00620 0.29 -1.1 99,800 - yaeP MCAODC_00620 0.29 -0.7 99,800 - yaeP MCAODC_00620 0.29 -0.7 99,800 - yaeP MCAODC_00620 0.29 -0.4 99,800 - yaeP MCAODC_00620 0.29 -0.3 99,800 - yaeP MCAODC_00620 0.29 -0.9 99,804 + yaeP MCAODC_00620 0.31 -0.8 99,804 + yaeP MCAODC_00620 0.31 +1.5 99,804 + yaeP MCAODC_00620 0.31 -0.0 99,805 - yaeP MCAODC_00620 0.32 -1.4 99,897 + yaeP MCAODC_00620 0.78 +1.6 99,897 + yaeP MCAODC_00620 0.78 +0.0 99,897 + yaeP MCAODC_00620 0.78 -0.5 99,928 - +0.3 99,946 + -1.1 99,946 + -2.0 99,946 + -0.1 99,946 + -0.4 99,946 + -0.6 99,947 - +0.3 99,947 - -0.2 99,970 + rof MCAODC_00625 0.16 -0.1 99,970 + rof MCAODC_00625 0.16 -1.2 99,970 + rof MCAODC_00625 0.16 -0.3 99,971 - rof MCAODC_00625 0.16 +0.0 100,004 + rof MCAODC_00625 0.29 +1.5 100,005 - rof MCAODC_00625 0.30 +1.3 100,005 - rof MCAODC_00625 0.30 +1.2 100,005 - rof MCAODC_00625 0.30 -1.4 100,005 - rof MCAODC_00625 0.30 +0.6 100,121 + rof MCAODC_00625 0.74 -0.6 100,122 - rof MCAODC_00625 0.74 -0.9 100,146 + rof MCAODC_00625 0.84 -0.1 100,213 + -0.6 100,224 - +0.3 100,242 + -0.3
Or see this region's nucleotide sequence