Experiment: Ying_Nucleobase14 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5698 and GFF5699 are separated by 7 nucleotides GFF5699 and GFF5700 are separated by 42 nucleotides GFF5700 and GFF5701 are separated by 9 nucleotides GFF5701 and GFF5702 overlap by 4 nucleotides
GFF5698 - Isochorismatase (EC 3.3.2.1), at 2,721,803 to 2,722,426
GFF5698
GFF5699 - hypothetical protein, at 2,722,434 to 2,723,183
GFF5699
GFF5700 - extracellular solute-binding protein, family 3, at 2,723,226 to 2,724,113
GFF5700
GFF5701 - Amino acid ABC transporter, permease protein, at 2,724,123 to 2,724,770
GFF5701
GFF5702 - Amino acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine, at 2,724,767 to 2,725,423
GFF5702
Position (kb)
2723
2724
2725 Strain fitness (log2 ratio)
-1
0
1 at 2722.776 kb on + strand, within GFF5699 at 2722.887 kb on + strand, within GFF5699 at 2723.497 kb on - strand, within GFF5700 at 2723.497 kb on - strand, within GFF5700 at 2723.569 kb on - strand, within GFF5700 at 2723.658 kb on + strand, within GFF5700 at 2723.659 kb on - strand, within GFF5700 at 2723.659 kb on - strand, within GFF5700 at 2723.659 kb on - strand, within GFF5700 at 2723.659 kb on - strand, within GFF5700 at 2723.835 kb on + strand, within GFF5700 at 2723.835 kb on + strand, within GFF5700 at 2723.956 kb on - strand, within GFF5700 at 2724.096 kb on + strand at 2724.277 kb on - strand, within GFF5701 at 2724.651 kb on + strand, within GFF5701 at 2724.690 kb on + strand, within GFF5701 at 2724.690 kb on + strand, within GFF5701 at 2724.690 kb on + strand, within GFF5701 at 2724.691 kb on - strand, within GFF5701 at 2724.691 kb on - strand, within GFF5701 at 2724.691 kb on - strand, within GFF5701 at 2725.059 kb on - strand, within GFF5702 at 2725.070 kb on + strand, within GFF5702
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1 remove 2,722,776 + GFF5699 0.46 -0.0 2,722,887 + GFF5699 0.60 +0.9 2,723,497 - GFF5700 0.31 -0.0 2,723,497 - GFF5700 0.31 -0.2 2,723,569 - GFF5700 0.39 +0.8 2,723,658 + GFF5700 0.49 -0.8 2,723,659 - GFF5700 0.49 +0.6 2,723,659 - GFF5700 0.49 +0.4 2,723,659 - GFF5700 0.49 -0.4 2,723,659 - GFF5700 0.49 +0.2 2,723,835 + GFF5700 0.69 -0.0 2,723,835 + GFF5700 0.69 +0.0 2,723,956 - GFF5700 0.82 +0.0 2,724,096 + -0.7 2,724,277 - GFF5701 0.24 +0.8 2,724,651 + GFF5701 0.81 +0.3 2,724,690 + GFF5701 0.88 -0.5 2,724,690 + GFF5701 0.88 +0.3 2,724,690 + GFF5701 0.88 +0.1 2,724,691 - GFF5701 0.88 +0.5 2,724,691 - GFF5701 0.88 -0.4 2,724,691 - GFF5701 0.88 +0.0 2,725,059 - GFF5702 0.44 -0.0 2,725,070 + GFF5702 0.46 +0.5
Or see this region's nucleotide sequence