Experiment: Ying_Nucleobase14 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF506 and GFF507 are separated by 3 nucleotides GFF507 and GFF508 overlap by 4 nucleotides GFF508 and GFF509 overlap by 4 nucleotides
GFF506 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 173,589 to 175,445
GFF506
GFF507 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 175,449 to 176,237
GFF507
GFF508 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 176,234 to 177,061
GFF508
GFF509 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 177,058 to 178,134
GFF509
Position (kb) 176
177
178 Strain fitness (log2 ratio) -1
0
1 at 175.451 kb on - strand at 175.594 kb on + strand, within GFF507 at 175.595 kb on - strand, within GFF507 at 175.601 kb on - strand, within GFF507 at 175.852 kb on + strand, within GFF507 at 175.853 kb on - strand, within GFF507 at 176.295 kb on + strand at 176.295 kb on + strand at 176.296 kb on - strand at 176.868 kb on + strand, within GFF508 at 176.895 kb on + strand, within GFF508 at 176.896 kb on - strand, within GFF508 at 177.425 kb on + strand, within GFF509 at 177.621 kb on - strand, within GFF509 at 177.765 kb on - strand, within GFF509 at 177.765 kb on - strand, within GFF509
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1 remove 175,451 - -1.2 175,594 + GFF507 0.18 +0.2 175,595 - GFF507 0.19 -0.2 175,601 - GFF507 0.19 +0.6 175,852 + GFF507 0.51 +0.8 175,853 - GFF507 0.51 -0.7 176,295 + +0.5 176,295 + +0.4 176,296 - -0.1 176,868 + GFF508 0.77 -0.9 176,895 + GFF508 0.80 +1.2 176,896 - GFF508 0.80 -0.9 177,425 + GFF509 0.34 -0.4 177,621 - GFF509 0.52 -0.3 177,765 - GFF509 0.66 -0.3 177,765 - GFF509 0.66 +0.3
Or see this region's nucleotide sequence