Experiment: Ying_Nucleobase14 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3561 and GFF3562 are separated by 64 nucleotides GFF3562 and GFF3563 overlap by 23 nucleotides GFF3563 and GFF3564 are separated by 103 nucleotides GFF3564 and GFF3565 are separated by 31 nucleotides
GFF3561 - Glutamate--cysteine ligase (EC 6.3.2.2), at 452,336 to 453,895
GFF3561
GFF3562 - Dienelactone hydrolase family, at 453,960 to 454,886
GFF3562
GFF3563 - NAD(P)H oxidoreductase YRKL (EC 1.6.99.-) @ Putative NADPH-quinone reductase (modulator of drug activity B) @ Flavodoxin 2, at 454,864 to 455,499
GFF3563
GFF3564 - Transcriptional regulator, TetR family, at 455,603 to 456,184
GFF3564
GFF3565 - hypothetical protein, at 456,216 to 456,686
GFF3565
Position (kb) 454
455
456 Strain fitness (log2 ratio) -2
-1
0
1
2 at 453.908 kb on - strand at 453.908 kb on - strand at 453.908 kb on - strand at 453.959 kb on + strand at 453.959 kb on + strand at 453.959 kb on + strand at 453.959 kb on + strand at 453.959 kb on + strand at 453.959 kb on + strand at 453.959 kb on + strand at 453.960 kb on - strand at 453.960 kb on - strand at 453.960 kb on - strand at 453.960 kb on - strand at 453.960 kb on - strand at 453.960 kb on - strand at 453.960 kb on - strand at 453.960 kb on - strand at 453.960 kb on - strand at 453.960 kb on - strand at 453.960 kb on - strand at 454.047 kb on + strand at 454.047 kb on + strand at 454.047 kb on + strand at 454.048 kb on - strand at 454.048 kb on - strand at 454.048 kb on - strand at 454.048 kb on - strand at 454.048 kb on - strand at 454.200 kb on + strand, within GFF3562 at 454.248 kb on + strand, within GFF3562 at 454.248 kb on + strand, within GFF3562 at 454.248 kb on + strand, within GFF3562 at 454.248 kb on + strand, within GFF3562 at 454.248 kb on + strand, within GFF3562 at 454.249 kb on - strand, within GFF3562 at 454.249 kb on - strand, within GFF3562 at 454.249 kb on - strand, within GFF3562 at 454.371 kb on + strand, within GFF3562 at 454.372 kb on - strand, within GFF3562 at 454.554 kb on + strand, within GFF3562 at 454.617 kb on + strand, within GFF3562 at 454.617 kb on + strand, within GFF3562 at 454.617 kb on + strand, within GFF3562 at 454.618 kb on - strand, within GFF3562 at 454.621 kb on - strand, within GFF3562 at 454.621 kb on - strand, within GFF3562 at 454.722 kb on + strand, within GFF3562 at 454.722 kb on + strand, within GFF3562 at 454.723 kb on - strand, within GFF3562 at 454.723 kb on - strand, within GFF3562 at 454.755 kb on + strand, within GFF3562 at 454.755 kb on + strand, within GFF3562 at 455.084 kb on + strand, within GFF3563 at 455.084 kb on + strand, within GFF3563 at 455.084 kb on + strand, within GFF3563 at 455.085 kb on - strand, within GFF3563 at 455.331 kb on - strand, within GFF3563 at 455.529 kb on + strand at 455.530 kb on - strand at 455.530 kb on - strand at 455.530 kb on - strand at 455.544 kb on - strand at 455.753 kb on + strand, within GFF3564 at 455.753 kb on + strand, within GFF3564 at 455.753 kb on + strand, within GFF3564 at 455.753 kb on + strand, within GFF3564 at 455.753 kb on + strand, within GFF3564 at 455.753 kb on + strand, within GFF3564 at 455.754 kb on - strand, within GFF3564 at 455.804 kb on + strand, within GFF3564 at 455.804 kb on + strand, within GFF3564 at 455.805 kb on - strand, within GFF3564 at 455.894 kb on + strand, within GFF3564 at 455.894 kb on + strand, within GFF3564 at 455.894 kb on + strand, within GFF3564 at 455.894 kb on + strand, within GFF3564 at 455.894 kb on + strand, within GFF3564 at 455.894 kb on + strand, within GFF3564 at 455.894 kb on + strand, within GFF3564 at 455.895 kb on - strand, within GFF3564 at 455.895 kb on - strand, within GFF3564 at 455.895 kb on - strand, within GFF3564 at 455.895 kb on - strand, within GFF3564 at 455.895 kb on - strand, within GFF3564 at 456.150 kb on - strand at 456.256 kb on + strand at 456.472 kb on + strand, within GFF3565 at 456.472 kb on + strand, within GFF3565 at 456.472 kb on + strand, within GFF3565 at 456.472 kb on + strand, within GFF3565 at 456.472 kb on + strand, within GFF3565 at 456.473 kb on - strand, within GFF3565 at 456.484 kb on + strand, within GFF3565 at 456.484 kb on + strand, within GFF3565 at 456.484 kb on + strand, within GFF3565
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1 remove 453,908 - -0.5 453,908 - -0.2 453,908 - +0.1 453,959 + +2.0 453,959 + -0.1 453,959 + +1.0 453,959 + +0.1 453,959 + -0.5 453,959 + -0.3 453,959 + +0.0 453,960 - +0.1 453,960 - +0.3 453,960 - +0.5 453,960 - -0.8 453,960 - -0.3 453,960 - +0.0 453,960 - +1.5 453,960 - -0.6 453,960 - -1.1 453,960 - +0.2 453,960 - +0.4 454,047 + +0.2 454,047 + -0.8 454,047 + -0.1 454,048 - +0.7 454,048 - +0.2 454,048 - -0.6 454,048 - -0.1 454,048 - -2.5 454,200 + GFF3562 0.26 +0.7 454,248 + GFF3562 0.31 -0.6 454,248 + GFF3562 0.31 -0.1 454,248 + GFF3562 0.31 +0.1 454,248 + GFF3562 0.31 +0.5 454,248 + GFF3562 0.31 -0.3 454,249 - GFF3562 0.31 -1.1 454,249 - GFF3562 0.31 +0.3 454,249 - GFF3562 0.31 -0.2 454,371 + GFF3562 0.44 -0.1 454,372 - GFF3562 0.44 -0.6 454,554 + GFF3562 0.64 -0.6 454,617 + GFF3562 0.71 -1.4 454,617 + GFF3562 0.71 -0.8 454,617 + GFF3562 0.71 +0.4 454,618 - GFF3562 0.71 -0.1 454,621 - GFF3562 0.71 +0.5 454,621 - GFF3562 0.71 +0.3 454,722 + GFF3562 0.82 +1.0 454,722 + GFF3562 0.82 +0.1 454,723 - GFF3562 0.82 -0.1 454,723 - GFF3562 0.82 -0.6 454,755 + GFF3562 0.86 +0.0 454,755 + GFF3562 0.86 +0.3 455,084 + GFF3563 0.35 -0.1 455,084 + GFF3563 0.35 -0.1 455,084 + GFF3563 0.35 -0.0 455,085 - GFF3563 0.35 -0.6 455,331 - GFF3563 0.73 -0.6 455,529 + -0.9 455,530 - -0.1 455,530 - +0.4 455,530 - +0.1 455,544 - +0.1 455,753 + GFF3564 0.26 -0.4 455,753 + GFF3564 0.26 +0.2 455,753 + GFF3564 0.26 +0.1 455,753 + GFF3564 0.26 -0.6 455,753 + GFF3564 0.26 +0.1 455,753 + GFF3564 0.26 +0.0 455,754 - GFF3564 0.26 +0.9 455,804 + GFF3564 0.35 +0.1 455,804 + GFF3564 0.35 +0.2 455,805 - GFF3564 0.35 -2.3 455,894 + GFF3564 0.50 -0.8 455,894 + GFF3564 0.50 -0.2 455,894 + GFF3564 0.50 +0.1 455,894 + GFF3564 0.50 -0.4 455,894 + GFF3564 0.50 +0.4 455,894 + GFF3564 0.50 -0.6 455,894 + GFF3564 0.50 -0.3 455,895 - GFF3564 0.50 +0.4 455,895 - GFF3564 0.50 +0.7 455,895 - GFF3564 0.50 +0.3 455,895 - GFF3564 0.50 -0.6 455,895 - GFF3564 0.50 +0.9 456,150 - -0.4 456,256 + -0.6 456,472 + GFF3565 0.54 +0.4 456,472 + GFF3565 0.54 -0.7 456,472 + GFF3565 0.54 -0.3 456,472 + GFF3565 0.54 -0.2 456,472 + GFF3565 0.54 -0.4 456,473 - GFF3565 0.55 -0.5 456,484 + GFF3565 0.57 -0.3 456,484 + GFF3565 0.57 -0.5 456,484 + GFF3565 0.57 -0.4
Or see this region's nucleotide sequence