Experiment: Ying_Nucleobase14 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2874 and GFF2875 are separated by 2 nucleotides GFF2875 and GFF2876 are separated by 100 nucleotides GFF2876 and GFF2877 are separated by 24 nucleotides
GFF2874 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 462,118 to 462,873
GFF2874
GFF2875 - Transcriptional regulator, LysR family, at 462,876 to 463,769
GFF2875
GFF2876 - Putative drug efflux protein, at 463,870 to 465,081
GFF2876
GFF2877 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274), at 465,106 to 465,918
GFF2877
Position (kb) 463
464
465
466 Strain fitness (log2 ratio) -2
-1
0
1 at 462.953 kb on - strand at 462.955 kb on + strand at 463.255 kb on + strand, within GFF2875 at 463.256 kb on - strand, within GFF2875 at 464.009 kb on - strand, within GFF2876 at 464.533 kb on + strand, within GFF2876 at 464.534 kb on - strand, within GFF2876 at 464.611 kb on + strand, within GFF2876 at 464.809 kb on + strand, within GFF2876 at 464.809 kb on + strand, within GFF2876 at 464.810 kb on - strand, within GFF2876 at 465.229 kb on + strand, within GFF2877 at 465.661 kb on + strand, within GFF2877 at 465.662 kb on - strand, within GFF2877 at 465.986 kb on - strand at 465.986 kb on - strand at 465.986 kb on - strand at 466.037 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1 remove 462,953 - -2.1 462,955 + +1.2 463,255 + GFF2875 0.42 -0.2 463,256 - GFF2875 0.43 -2.6 464,009 - GFF2876 0.11 +0.5 464,533 + GFF2876 0.55 -1.1 464,534 - GFF2876 0.55 +0.4 464,611 + GFF2876 0.61 -0.0 464,809 + GFF2876 0.77 -0.1 464,809 + GFF2876 0.77 -0.1 464,810 - GFF2876 0.78 -1.0 465,229 + GFF2877 0.15 +0.9 465,661 + GFF2877 0.68 +0.2 465,662 - GFF2877 0.68 +0.6 465,986 - +0.3 465,986 - +0.3 465,986 - +0.9 466,037 + -0.8
Or see this region's nucleotide sequence