Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF14

Experiment: Ying_Nucleobase14 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF12 and GFF13 are separated by 32 nucleotidesGFF13 and GFF14 are separated by 10 nucleotidesGFF14 and GFF15 overlap by 4 nucleotidesGFF15 and GFF16 are separated by 42 nucleotidesGFF16 and GFF17 are separated by 1 nucleotides GFF12 - Acetolactate synthase large subunit (EC 2.2.1.6), at 9,626 to 10,285 GFF12 GFF13 - Aldehyde dehydrogenase (EC 1.2.1.3), at 10,318 to 10,647 GFF13 GFF14 - Aldehyde dehydrogenase (EC 1.2.1.3), at 10,658 to 11,764 GFF14 GFF15 - Aldehyde dehydrogenase (EC 1.2.1.3), at 11,761 to 11,874 GFF15 GFF16 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 11,917 to 12,696 GFF16 GFF17 - LysR family transcriptional regulator YfeR, at 12,698 to 13,600 GFF17 Position (kb) 10 11 12Strain fitness (log2 ratio) -1 0 1at 11.331 kb on - strand, within GFF14at 12.396 kb on + strand, within GFF16at 12.616 kb on + strand, within GFF16at 12.641 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1
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11,331 - GFF14 0.61 +0.6
12,396 + GFF16 0.61 +0.4
12,616 + GFF16 0.90 -1.1
12,641 - -0.7

Or see this region's nucleotide sequence