Experiment: Ying_Nucleobase14 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF119 and GFF120 are separated by 102 nucleotides GFF120 and GFF121 are separated by 591 nucleotides
GFF119 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 130,261 to 131,565
GFF119
GFF120 - Ribonuclease E (EC 3.1.26.12), at 131,668 to 134,763
GFF120
GFF121 - Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70), at 135,355 to 136,344
GFF121
Position (kb) 131
132
133
134
135 Strain fitness (log2 ratio) -1
0
1 at 131.073 kb on + strand, within GFF119 at 131.074 kb on - strand, within GFF119 at 131.074 kb on - strand, within GFF119 at 131.371 kb on + strand, within GFF119 at 131.372 kb on - strand, within GFF119 at 131.372 kb on - strand, within GFF119 at 131.372 kb on - strand, within GFF119 at 132.149 kb on + strand, within GFF120 at 132.150 kb on - strand, within GFF120 at 132.150 kb on - strand, within GFF120 at 132.150 kb on - strand, within GFF120 at 132.171 kb on - strand, within GFF120 at 134.948 kb on - strand at 135.287 kb on - strand at 135.287 kb on - strand at 135.314 kb on + strand at 135.314 kb on + strand at 135.315 kb on - strand at 135.344 kb on + strand at 135.344 kb on + strand at 135.345 kb on - strand at 135.345 kb on - strand at 135.462 kb on + strand, within GFF121 at 135.462 kb on + strand, within GFF121 at 135.462 kb on + strand, within GFF121 at 135.462 kb on + strand, within GFF121 at 135.462 kb on + strand, within GFF121 at 135.462 kb on + strand, within GFF121 at 135.462 kb on + strand, within GFF121 at 135.463 kb on - strand, within GFF121 at 135.463 kb on - strand, within GFF121 at 135.463 kb on - strand, within GFF121 at 135.475 kb on + strand, within GFF121 at 135.475 kb on + strand, within GFF121 at 135.475 kb on + strand, within GFF121 at 135.475 kb on + strand, within GFF121 at 135.475 kb on + strand, within GFF121 at 135.476 kb on - strand, within GFF121 at 135.476 kb on - strand, within GFF121 at 135.476 kb on - strand, within GFF121 at 135.476 kb on - strand, within GFF121 at 135.476 kb on - strand, within GFF121 at 135.476 kb on - strand, within GFF121 at 135.476 kb on - strand, within GFF121 at 135.689 kb on + strand, within GFF121 at 135.689 kb on + strand, within GFF121 at 135.689 kb on + strand, within GFF121 at 135.689 kb on + strand, within GFF121 at 135.689 kb on + strand, within GFF121 at 135.689 kb on + strand, within GFF121 at 135.690 kb on - strand, within GFF121 at 135.690 kb on - strand, within GFF121 at 135.690 kb on - strand, within GFF121 at 135.690 kb on - strand, within GFF121 at 135.690 kb on - strand, within GFF121 at 135.690 kb on - strand, within GFF121
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1 remove 131,073 + GFF119 0.62 -0.4 131,074 - GFF119 0.62 -0.1 131,074 - GFF119 0.62 -0.4 131,371 + GFF119 0.85 -0.2 131,372 - GFF119 0.85 -0.4 131,372 - GFF119 0.85 -0.5 131,372 - GFF119 0.85 +0.1 132,149 + GFF120 0.16 -0.3 132,150 - GFF120 0.16 +1.0 132,150 - GFF120 0.16 +0.8 132,150 - GFF120 0.16 -1.7 132,171 - GFF120 0.16 +0.8 134,948 - -1.3 135,287 - +0.3 135,287 - +0.7 135,314 + +1.0 135,314 + +0.7 135,315 - -0.4 135,344 + -0.6 135,344 + +0.6 135,345 - +0.7 135,345 - +0.3 135,462 + GFF121 0.11 -0.4 135,462 + GFF121 0.11 +0.0 135,462 + GFF121 0.11 +0.4 135,462 + GFF121 0.11 -0.4 135,462 + GFF121 0.11 +0.1 135,462 + GFF121 0.11 -0.3 135,462 + GFF121 0.11 +0.5 135,463 - GFF121 0.11 -0.1 135,463 - GFF121 0.11 +0.7 135,463 - GFF121 0.11 +1.2 135,475 + GFF121 0.12 +1.5 135,475 + GFF121 0.12 +0.5 135,475 + GFF121 0.12 -0.5 135,475 + GFF121 0.12 -0.7 135,475 + GFF121 0.12 +0.3 135,476 - GFF121 0.12 +1.1 135,476 - GFF121 0.12 +0.7 135,476 - GFF121 0.12 +0.0 135,476 - GFF121 0.12 +0.3 135,476 - GFF121 0.12 +0.3 135,476 - GFF121 0.12 +0.4 135,476 - GFF121 0.12 +0.5 135,689 + GFF121 0.34 -0.3 135,689 + GFF121 0.34 -0.1 135,689 + GFF121 0.34 +0.8 135,689 + GFF121 0.34 +0.2 135,689 + GFF121 0.34 +0.5 135,689 + GFF121 0.34 -1.5 135,690 - GFF121 0.34 +0.8 135,690 - GFF121 0.34 -0.9 135,690 - GFF121 0.34 +0.5 135,690 - GFF121 0.34 -0.7 135,690 - GFF121 0.34 -0.8 135,690 - GFF121 0.34 +0.3
Or see this region's nucleotide sequence