Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_25835 and MCAODC_25840 overlap by 4 nucleotides MCAODC_25840 and MCAODC_25845 overlap by 4 nucleotides MCAODC_25845 and MCAODC_25850 are separated by 98 nucleotides MCAODC_25850 and MCAODC_25855 are separated by 210 nucleotides MCAODC_25855 and MCAODC_25860 are separated by 242 nucleotides MCAODC_25860 and int are separated by 235 nucleotides
MCAODC_25830: MCAODC_25830 - cruciferin, at 1,886,024 to 1,886,206
_25830
MCAODC_25835: MCAODC_25835 - DUF1382 domain-containing protein, at 1,886,179 to 1,886,370
_25835
MCAODC_25840: MCAODC_25840 - DUF1382 domain-containing protein, at 1,886,367 to 1,886,450
_25840
MCAODC_25845: MCAODC_25845 - Cell division protein ZapA, at 1,886,447 to 1,886,662
_25845
MCAODC_25850: MCAODC_25850 - conjugal transfer protein TraR, at 1,886,761 to 1,886,982
_25850
MCAODC_25855: MCAODC_25855 - hypothetical protein, at 1,887,193 to 1,887,795
_25855
MCAODC_25860: MCAODC_25860 - hypothetical protein, at 1,888,038 to 1,888,205
_25860
MCAODC_25865: int - Integrase, at 1,888,441 to 1,889,511
int
Position (kb) 1887
1888 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1886.292 kb on + strand, within MCAODC_25835 at 1886.292 kb on + strand, within MCAODC_25835 at 1886.408 kb on - strand, within MCAODC_25840 at 1886.421 kb on - strand, within MCAODC_25840 at 1887.117 kb on - strand at 1887.165 kb on - strand at 1887.183 kb on - strand at 1887.190 kb on - strand at 1887.271 kb on - strand, within MCAODC_25855 at 1887.379 kb on - strand, within MCAODC_25855 at 1887.470 kb on - strand, within MCAODC_25855 at 1887.497 kb on + strand, within MCAODC_25855 at 1887.559 kb on + strand, within MCAODC_25855 at 1887.572 kb on - strand, within MCAODC_25855 at 1887.607 kb on - strand, within MCAODC_25855 at 1887.878 kb on + strand at 1887.949 kb on + strand at 1888.036 kb on - strand at 1888.144 kb on + strand, within MCAODC_25860 at 1888.167 kb on - strand, within MCAODC_25860 at 1888.248 kb on + strand at 1888.248 kb on + strand at 1888.248 kb on + strand at 1888.248 kb on + strand at 1888.248 kb on + strand at 1888.249 kb on - strand at 1888.249 kb on - strand at 1888.249 kb on - strand at 1888.366 kb on - strand at 1888.366 kb on - strand at 1888.475 kb on + strand at 1888.489 kb on + strand at 1888.489 kb on + strand at 1888.490 kb on - strand at 1888.491 kb on + strand at 1888.491 kb on + strand at 1888.492 kb on - strand at 1888.492 kb on - strand at 1888.493 kb on + strand at 1888.507 kb on + strand at 1888.511 kb on - strand at 1888.517 kb on - strand at 1888.517 kb on - strand at 1888.596 kb on + strand, within int at 1888.653 kb on + strand, within int at 1888.654 kb on - strand, within int at 1888.662 kb on + strand, within int at 1888.662 kb on + strand, within int at 1888.668 kb on - strand, within int at 1888.668 kb on - strand, within int at 1888.738 kb on + strand, within int at 1888.738 kb on + strand, within int at 1888.739 kb on - strand, within int
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,886,292 + MCAODC_25835 0.59 +0.4 1,886,292 + MCAODC_25835 0.59 -0.3 1,886,408 - MCAODC_25840 0.49 -1.3 1,886,421 - MCAODC_25840 0.64 +1.2 1,887,117 - -0.1 1,887,165 - +0.0 1,887,183 - +0.3 1,887,190 - +1.3 1,887,271 - MCAODC_25855 0.13 +0.0 1,887,379 - MCAODC_25855 0.31 -0.7 1,887,470 - MCAODC_25855 0.46 +1.9 1,887,497 + MCAODC_25855 0.50 +0.4 1,887,559 + MCAODC_25855 0.61 +0.6 1,887,572 - MCAODC_25855 0.63 -1.7 1,887,607 - MCAODC_25855 0.69 -0.6 1,887,878 + -0.7 1,887,949 + +0.9 1,888,036 - -0.5 1,888,144 + MCAODC_25860 0.63 -0.1 1,888,167 - MCAODC_25860 0.77 +0.9 1,888,248 + +0.3 1,888,248 + +0.6 1,888,248 + -2.0 1,888,248 + -0.9 1,888,248 + -0.7 1,888,249 - +0.3 1,888,249 - -0.3 1,888,249 - +0.9 1,888,366 - +1.1 1,888,366 - -0.5 1,888,475 + +1.6 1,888,489 + -0.1 1,888,489 + -0.8 1,888,490 - +0.4 1,888,491 + -0.4 1,888,491 + +0.6 1,888,492 - -0.3 1,888,492 - +0.8 1,888,493 + -0.1 1,888,507 + -0.1 1,888,511 - -0.6 1,888,517 - -0.5 1,888,517 - -0.3 1,888,596 + int MCAODC_25865 0.14 -0.1 1,888,653 + int MCAODC_25865 0.20 -0.4 1,888,654 - int MCAODC_25865 0.20 +0.8 1,888,662 + int MCAODC_25865 0.21 -1.1 1,888,662 + int MCAODC_25865 0.21 -0.1 1,888,668 - int MCAODC_25865 0.21 -0.1 1,888,668 - int MCAODC_25865 0.21 +1.4 1,888,738 + int MCAODC_25865 0.28 -0.3 1,888,738 + int MCAODC_25865 0.28 -0.4 1,888,739 - int MCAODC_25865 0.28 -0.0
Or see this region's nucleotide sequence