Strain Fitness in Escherichia coli ECRC101 around MCAODC_23140

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmdtG and msyB are separated by 82 nucleotidesmsyB and yceK are separated by 0 nucleotidesyceK and mdoH are separated by 172 nucleotides MCAODC_23135: mdtG - multidrug efflux MFS transporter MdtG, at 1,366,103 to 1,367,074 mdtG MCAODC_23140: msyB - MysB family protein, at 1,367,157 to 1,367,531 msyB MCAODC_23145: yceK - YceK/YidQ family lipoprotein, at 1,367,532 to 1,367,759 yceK MCAODC_23155: mdoH - glucans biosynthesis glucosyltransferase MdoH, at 1,367,932 to 1,370,445 mdoH Position (kb) 1367 1368Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1366.261 kb on + strand, within mdtGat 1366.376 kb on - strand, within mdtGat 1366.395 kb on + strand, within mdtGat 1366.492 kb on + strand, within mdtGat 1366.580 kb on + strand, within mdtGat 1366.581 kb on - strand, within mdtGat 1366.610 kb on - strand, within mdtGat 1366.766 kb on + strand, within mdtGat 1366.767 kb on - strand, within mdtGat 1366.767 kb on - strand, within mdtGat 1366.767 kb on - strand, within mdtGat 1366.889 kb on - strand, within mdtGat 1366.898 kb on + strand, within mdtGat 1366.899 kb on - strand, within mdtGat 1366.984 kb on - strandat 1367.004 kb on + strandat 1367.040 kb on + strandat 1367.089 kb on + strandat 1367.090 kb on - strandat 1367.113 kb on + strandat 1367.166 kb on + strandat 1367.351 kb on + strand, within msyBat 1367.351 kb on + strand, within msyBat 1367.435 kb on + strand, within msyBat 1367.435 kb on + strand, within msyBat 1367.435 kb on + strand, within msyBat 1367.435 kb on + strand, within msyBat 1367.435 kb on + strand, within msyBat 1367.436 kb on - strand, within msyBat 1367.440 kb on + strand, within msyBat 1367.440 kb on + strand, within msyBat 1367.440 kb on + strand, within msyBat 1367.440 kb on + strand, within msyBat 1367.529 kb on + strandat 1367.530 kb on - strandat 1367.533 kb on + strandat 1367.617 kb on + strand, within yceKat 1367.617 kb on + strand, within yceKat 1367.617 kb on + strand, within yceKat 1367.617 kb on + strand, within yceKat 1367.618 kb on - strand, within yceKat 1367.662 kb on + strand, within yceKat 1367.662 kb on + strand, within yceKat 1367.691 kb on - strand, within yceKat 1367.751 kb on + strandat 1367.991 kb on - strandat 1367.991 kb on - strandat 1367.994 kb on - strandat 1367.994 kb on - strandat 1367.994 kb on - strandat 1368.011 kb on + strandat 1368.012 kb on - strandat 1368.208 kb on - strand, within mdoHat 1368.235 kb on - strand, within mdoHat 1368.235 kb on - strand, within mdoHat 1368.296 kb on - strand, within mdoHat 1368.296 kb on - strand, within mdoHat 1368.482 kb on - strand, within mdoH

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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1,366,261 + mdtG MCAODC_23135 0.16 +1.2
1,366,376 - mdtG MCAODC_23135 0.28 +0.4
1,366,395 + mdtG MCAODC_23135 0.30 -2.1
1,366,492 + mdtG MCAODC_23135 0.40 +0.0
1,366,580 + mdtG MCAODC_23135 0.49 -1.0
1,366,581 - mdtG MCAODC_23135 0.49 +0.2
1,366,610 - mdtG MCAODC_23135 0.52 +2.2
1,366,766 + mdtG MCAODC_23135 0.68 -0.4
1,366,767 - mdtG MCAODC_23135 0.68 -1.1
1,366,767 - mdtG MCAODC_23135 0.68 +0.0
1,366,767 - mdtG MCAODC_23135 0.68 +2.0
1,366,889 - mdtG MCAODC_23135 0.81 +1.8
1,366,898 + mdtG MCAODC_23135 0.82 +1.8
1,366,899 - mdtG MCAODC_23135 0.82 +0.5
1,366,984 - +0.8
1,367,004 + -1.5
1,367,040 + +0.6
1,367,089 + +1.2
1,367,090 - -1.0
1,367,113 + +1.8
1,367,166 + +0.5
1,367,351 + msyB MCAODC_23140 0.52 -1.3
1,367,351 + msyB MCAODC_23140 0.52 +0.1
1,367,435 + msyB MCAODC_23140 0.74 +1.8
1,367,435 + msyB MCAODC_23140 0.74 -0.9
1,367,435 + msyB MCAODC_23140 0.74 -1.4
1,367,435 + msyB MCAODC_23140 0.74 +0.2
1,367,435 + msyB MCAODC_23140 0.74 +1.4
1,367,436 - msyB MCAODC_23140 0.74 -0.9
1,367,440 + msyB MCAODC_23140 0.75 -0.5
1,367,440 + msyB MCAODC_23140 0.75 +1.1
1,367,440 + msyB MCAODC_23140 0.75 +0.2
1,367,440 + msyB MCAODC_23140 0.75 -1.0
1,367,529 + +0.8
1,367,530 - +2.8
1,367,533 + +0.8
1,367,617 + yceK MCAODC_23145 0.37 +1.8
1,367,617 + yceK MCAODC_23145 0.37 -0.2
1,367,617 + yceK MCAODC_23145 0.37 +1.0
1,367,617 + yceK MCAODC_23145 0.37 +1.5
1,367,618 - yceK MCAODC_23145 0.38 -0.6
1,367,662 + yceK MCAODC_23145 0.57 +1.1
1,367,662 + yceK MCAODC_23145 0.57 +4.1
1,367,691 - yceK MCAODC_23145 0.70 -0.6
1,367,751 + +0.8
1,367,991 - +1.4
1,367,991 - -0.2
1,367,994 - +0.2
1,367,994 - +0.6
1,367,994 - -1.3
1,368,011 + -0.2
1,368,012 - +0.2
1,368,208 - mdoH MCAODC_23155 0.11 +0.2
1,368,235 - mdoH MCAODC_23155 0.12 -0.7
1,368,235 - mdoH MCAODC_23155 0.12 -1.6
1,368,296 - mdoH MCAODC_23155 0.14 +1.8
1,368,296 - mdoH MCAODC_23155 0.14 -0.2
1,368,482 - mdoH MCAODC_23155 0.22 +1.5

Or see this region's nucleotide sequence