Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfaL and ubiG are separated by 140 nucleotides ubiG and gyrA are separated by 146 nucleotides
MCAODC_14175: yfaL - AIDA-I family autotransporter adhesin YfaL/EhaC, at 2,777,768 to 2,781,520
yfaL
MCAODC_14180: ubiG - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 2,781,661 to 2,782,383
ubiG
MCAODC_14185: gyrA - DNA topoisomerase (ATP-hydrolyzing) subunit A, at 2,782,530 to 2,785,157
gyrA
Position (kb)
2781
2782
2783 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2780.683 kb on + strand, within yfaL at 2780.684 kb on - strand, within yfaL at 2780.684 kb on - strand, within yfaL at 2780.733 kb on + strand, within yfaL at 2780.754 kb on - strand, within yfaL at 2780.800 kb on + strand, within yfaL at 2780.800 kb on + strand, within yfaL at 2780.800 kb on + strand, within yfaL at 2780.801 kb on - strand, within yfaL at 2780.801 kb on - strand, within yfaL at 2781.027 kb on - strand, within yfaL at 2781.037 kb on + strand, within yfaL at 2781.037 kb on + strand, within yfaL at 2781.037 kb on + strand, within yfaL at 2781.078 kb on - strand, within yfaL at 2781.094 kb on + strand, within yfaL at 2781.241 kb on + strand at 2781.261 kb on + strand at 2781.295 kb on - strand at 2781.303 kb on + strand at 2781.304 kb on - strand at 2781.304 kb on - strand at 2781.315 kb on - strand at 2781.362 kb on - strand at 2781.418 kb on + strand at 2781.519 kb on - strand at 2781.539 kb on + strand at 2781.540 kb on - strand at 2781.540 kb on - strand at 2781.557 kb on - strand at 2781.590 kb on - strand at 2781.598 kb on + strand at 2781.599 kb on - strand at 2781.666 kb on + strand at 2782.394 kb on - strand at 2782.421 kb on - strand at 2782.422 kb on + strand at 2782.423 kb on - strand at 2782.423 kb on - strand at 2782.423 kb on - strand at 2782.442 kb on + strand at 2782.442 kb on + strand at 2782.443 kb on - strand at 2782.451 kb on + strand at 2782.451 kb on + strand at 2782.451 kb on + strand at 2782.451 kb on + strand at 2782.451 kb on + strand at 2782.895 kb on + strand, within gyrA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,780,683 + yfaL MCAODC_14175 0.78 -0.2 2,780,684 - yfaL MCAODC_14175 0.78 -0.4 2,780,684 - yfaL MCAODC_14175 0.78 -0.2 2,780,733 + yfaL MCAODC_14175 0.79 -0.9 2,780,754 - yfaL MCAODC_14175 0.80 +1.1 2,780,800 + yfaL MCAODC_14175 0.81 +1.6 2,780,800 + yfaL MCAODC_14175 0.81 +1.5 2,780,800 + yfaL MCAODC_14175 0.81 +0.0 2,780,801 - yfaL MCAODC_14175 0.81 -1.4 2,780,801 - yfaL MCAODC_14175 0.81 +1.0 2,781,027 - yfaL MCAODC_14175 0.87 +0.9 2,781,037 + yfaL MCAODC_14175 0.87 +0.9 2,781,037 + yfaL MCAODC_14175 0.87 +0.4 2,781,037 + yfaL MCAODC_14175 0.87 -0.1 2,781,078 - yfaL MCAODC_14175 0.88 +0.9 2,781,094 + yfaL MCAODC_14175 0.89 +0.1 2,781,241 + +0.3 2,781,261 + -0.1 2,781,295 - +0.3 2,781,303 + -0.7 2,781,304 - -0.1 2,781,304 - +0.9 2,781,315 - +0.9 2,781,362 - +2.9 2,781,418 + -0.6 2,781,519 - -0.1 2,781,539 + +1.9 2,781,540 - +0.6 2,781,540 - -0.9 2,781,557 - -1.3 2,781,590 - +1.3 2,781,598 + +0.1 2,781,599 - +0.4 2,781,666 + +1.6 2,782,394 - +1.9 2,782,421 - +0.1 2,782,422 + -0.2 2,782,423 - -0.2 2,782,423 - +0.3 2,782,423 - -0.4 2,782,442 + -1.9 2,782,442 + -0.4 2,782,443 - -1.6 2,782,451 + -1.6 2,782,451 + -0.5 2,782,451 + -0.5 2,782,451 + +2.5 2,782,451 + +0.1 2,782,895 + gyrA MCAODC_14185 0.14 +3.2
Or see this region's nucleotide sequence