Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mlaC and mlaC overlap by 1 nucleotides mlaC and ibaG are separated by 144 nucleotides ibaG and murA are separated by 54 nucleotides murA and sfsB are separated by 47 nucleotides sfsB and ispB are separated by 228 nucleotides
MCAODC_08510: mlaC - phospholipid-binding protein MlaC, at 1,705,951 to 1,706,586
mlaC
MCAODC_08515: mlaC - phospholipid-binding protein MlaC, at 1,706,586 to 1,706,879
mlaC
MCAODC_08520: ibaG - BolA family iron metabolism protein IbaG, at 1,707,024 to 1,707,293
ibaG
MCAODC_08525: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 1,707,348 to 1,708,607
murA
MCAODC_08530: sfsB - DNA-binding transcriptional regulator SfsB, at 1,708,655 to 1,708,933
sfsB
MCAODC_08535: ispB - octaprenyl diphosphate synthase, at 1,709,162 to 1,710,133
ispB
Position (kb)
1707
1708
1709 Strain fitness (log2 ratio)
-2
-1
0
1 at 1706.440 kb on + strand, within mlaC at 1706.466 kb on + strand, within mlaC at 1706.467 kb on - strand, within mlaC at 1706.504 kb on + strand, within mlaC at 1706.504 kb on + strand, within mlaC at 1706.508 kb on + strand, within mlaC at 1706.508 kb on + strand, within mlaC at 1706.508 kb on + strand, within mlaC at 1706.509 kb on - strand, within mlaC at 1706.518 kb on - strand, within mlaC at 1706.702 kb on + strand, within mlaC at 1706.702 kb on + strand, within mlaC at 1706.703 kb on - strand, within mlaC at 1706.703 kb on - strand, within mlaC at 1706.703 kb on - strand, within mlaC at 1706.717 kb on + strand, within mlaC at 1706.718 kb on - strand, within mlaC at 1706.738 kb on + strand, within mlaC at 1706.738 kb on + strand, within mlaC at 1706.816 kb on - strand, within mlaC at 1706.829 kb on + strand, within mlaC at 1706.829 kb on + strand, within mlaC at 1706.829 kb on + strand, within mlaC at 1706.829 kb on + strand, within mlaC at 1706.830 kb on - strand, within mlaC at 1706.830 kb on - strand, within mlaC at 1706.830 kb on - strand, within mlaC at 1706.830 kb on - strand, within mlaC at 1706.830 kb on - strand, within mlaC at 1706.830 kb on - strand, within mlaC at 1706.831 kb on + strand, within mlaC at 1706.847 kb on + strand, within mlaC at 1706.847 kb on + strand, within mlaC at 1706.849 kb on + strand, within mlaC at 1706.952 kb on + strand at 1706.972 kb on + strand at 1706.973 kb on - strand at 1706.976 kb on + strand at 1706.997 kb on + strand at 1707.018 kb on + strand at 1707.018 kb on + strand at 1707.018 kb on + strand at 1707.047 kb on + strand at 1707.204 kb on + strand, within ibaG at 1707.204 kb on + strand, within ibaG at 1707.204 kb on + strand, within ibaG at 1707.204 kb on + strand, within ibaG at 1707.204 kb on + strand, within ibaG at 1707.206 kb on + strand, within ibaG at 1707.246 kb on + strand, within ibaG at 1707.246 kb on + strand, within ibaG at 1707.246 kb on + strand, within ibaG at 1707.246 kb on + strand, within ibaG at 1707.246 kb on + strand, within ibaG at 1707.296 kb on + strand at 1708.606 kb on - strand at 1708.674 kb on + strand at 1708.674 kb on + strand at 1708.674 kb on + strand at 1708.674 kb on + strand at 1708.675 kb on - strand at 1708.679 kb on - strand at 1708.679 kb on - strand at 1708.777 kb on + strand, within sfsB at 1708.777 kb on + strand, within sfsB at 1708.925 kb on - strand at 1708.925 kb on - strand at 1708.949 kb on - strand at 1708.954 kb on - strand at 1708.994 kb on + strand at 1708.995 kb on - strand at 1708.996 kb on + strand at 1709.032 kb on - strand at 1709.065 kb on + strand at 1709.065 kb on + strand at 1709.065 kb on + strand at 1709.065 kb on + strand at 1709.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,706,440 + mlaC MCAODC_08510 0.77 -0.4 1,706,466 + mlaC MCAODC_08510 0.81 -0.9 1,706,467 - mlaC MCAODC_08510 0.81 -0.1 1,706,504 + mlaC MCAODC_08510 0.87 -1.5 1,706,504 + mlaC MCAODC_08510 0.87 +1.1 1,706,508 + mlaC MCAODC_08510 0.88 -0.7 1,706,508 + mlaC MCAODC_08510 0.88 -1.4 1,706,508 + mlaC MCAODC_08510 0.88 -1.4 1,706,509 - mlaC MCAODC_08510 0.88 -0.8 1,706,518 - mlaC MCAODC_08510 0.89 -1.2 1,706,702 + mlaC MCAODC_08515 0.39 -0.3 1,706,702 + mlaC MCAODC_08515 0.39 +1.4 1,706,703 - mlaC MCAODC_08515 0.40 -0.9 1,706,703 - mlaC MCAODC_08515 0.40 +1.1 1,706,703 - mlaC MCAODC_08515 0.40 -0.9 1,706,717 + mlaC MCAODC_08515 0.45 -2.1 1,706,718 - mlaC MCAODC_08515 0.45 -1.6 1,706,738 + mlaC MCAODC_08515 0.52 +0.1 1,706,738 + mlaC MCAODC_08515 0.52 -1.8 1,706,816 - mlaC MCAODC_08515 0.78 -1.6 1,706,829 + mlaC MCAODC_08515 0.83 -1.3 1,706,829 + mlaC MCAODC_08515 0.83 -0.9 1,706,829 + mlaC MCAODC_08515 0.83 -0.9 1,706,829 + mlaC MCAODC_08515 0.83 -0.8 1,706,830 - mlaC MCAODC_08515 0.83 +1.1 1,706,830 - mlaC MCAODC_08515 0.83 -0.6 1,706,830 - mlaC MCAODC_08515 0.83 +1.7 1,706,830 - mlaC MCAODC_08515 0.83 -0.2 1,706,830 - mlaC MCAODC_08515 0.83 +0.5 1,706,830 - mlaC MCAODC_08515 0.83 +0.1 1,706,831 + mlaC MCAODC_08515 0.83 +1.1 1,706,847 + mlaC MCAODC_08515 0.89 -1.9 1,706,847 + mlaC MCAODC_08515 0.89 -1.1 1,706,849 + mlaC MCAODC_08515 0.89 -0.3 1,706,952 + +1.5 1,706,972 + +0.6 1,706,973 - +1.3 1,706,976 + -1.9 1,706,997 + -0.9 1,707,018 + +0.3 1,707,018 + +0.3 1,707,018 + -0.5 1,707,047 + -1.1 1,707,204 + ibaG MCAODC_08520 0.67 -0.8 1,707,204 + ibaG MCAODC_08520 0.67 -1.4 1,707,204 + ibaG MCAODC_08520 0.67 -0.3 1,707,204 + ibaG MCAODC_08520 0.67 +0.5 1,707,204 + ibaG MCAODC_08520 0.67 -1.5 1,707,206 + ibaG MCAODC_08520 0.67 +1.1 1,707,246 + ibaG MCAODC_08520 0.82 +1.1 1,707,246 + ibaG MCAODC_08520 0.82 +0.6 1,707,246 + ibaG MCAODC_08520 0.82 -1.4 1,707,246 + ibaG MCAODC_08520 0.82 +0.6 1,707,246 + ibaG MCAODC_08520 0.82 +1.3 1,707,296 + -0.6 1,708,606 - +0.7 1,708,674 + -0.3 1,708,674 + -0.5 1,708,674 + +0.1 1,708,674 + -2.1 1,708,675 - +0.5 1,708,679 - +0.9 1,708,679 - +0.2 1,708,777 + sfsB MCAODC_08530 0.44 +0.9 1,708,777 + sfsB MCAODC_08530 0.44 -0.4 1,708,925 - -0.6 1,708,925 - +0.8 1,708,949 - -0.4 1,708,954 - -0.7 1,708,994 + -0.2 1,708,995 - -0.3 1,708,996 + -0.3 1,709,032 - -0.8 1,709,065 + -0.7 1,709,065 + +0.8 1,709,065 + +1.1 1,709,065 + +0.3 1,709,120 - +0.7
Or see this region's nucleotide sequence