Strain Fitness in Escherichia coli ECRC101 around MCAODC_08045

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntzntR and arfA are separated by 55 nucleotidesarfA and mscL overlap by 4 nucleotidesmscL and trkA are separated by 129 nucleotidestrkA and rsmB are separated by 21 nucleotides MCAODC_08030: zntR - Zn(2+)-responsive transcriptional regulator, at 1,612,053 to 1,612,478 zntR MCAODC_08035: arfA - alternative ribosome-rescue factor ArfA, at 1,612,534 to 1,612,752 arfA MCAODC_08040: mscL - large-conductance mechanosensitive channel protein MscL, at 1,612,749 to 1,613,159 mscL MCAODC_08045: trkA - Trk system potassium transporter TrkA, at 1,613,289 to 1,614,665 trkA MCAODC_08050: rsmB - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 1,614,687 to 1,615,976 rsmB Position (kb) 1613 1614 1615Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1612.359 kb on - strand, within zntRat 1612.410 kb on - strand, within zntRat 1612.410 kb on - strand, within zntRat 1612.419 kb on + strand, within zntRat 1612.420 kb on - strand, within zntRat 1612.498 kb on + strandat 1612.498 kb on + strandat 1612.498 kb on + strandat 1612.498 kb on + strandat 1612.498 kb on + strandat 1612.499 kb on - strandat 1612.516 kb on + strandat 1612.551 kb on + strandat 1612.551 kb on + strandat 1612.551 kb on + strandat 1612.551 kb on + strandat 1612.552 kb on - strandat 1612.554 kb on + strandat 1612.554 kb on + strandat 1612.580 kb on + strand, within arfAat 1612.581 kb on - strand, within arfAat 1612.590 kb on - strand, within arfAat 1612.592 kb on + strand, within arfAat 1612.592 kb on + strand, within arfAat 1612.592 kb on + strand, within arfAat 1612.592 kb on + strand, within arfAat 1612.592 kb on + strand, within arfAat 1612.592 kb on + strand, within arfAat 1612.629 kb on + strand, within arfAat 1612.648 kb on + strand, within arfAat 1612.648 kb on + strand, within arfAat 1612.649 kb on - strand, within arfAat 1612.649 kb on - strand, within arfAat 1612.649 kb on - strand, within arfAat 1612.707 kb on - strand, within arfAat 1612.750 kb on + strandat 1612.751 kb on - strandat 1612.751 kb on - strandat 1612.751 kb on - strandat 1612.800 kb on + strand, within mscLat 1612.800 kb on + strand, within mscLat 1612.801 kb on - strand, within mscLat 1612.801 kb on - strand, within mscLat 1612.801 kb on - strand, within mscLat 1612.801 kb on - strand, within mscLat 1612.811 kb on + strand, within mscLat 1612.811 kb on + strand, within mscLat 1612.812 kb on - strand, within mscLat 1612.812 kb on - strand, within mscLat 1612.866 kb on + strand, within mscLat 1612.866 kb on + strand, within mscLat 1612.867 kb on - strand, within mscLat 1612.871 kb on + strand, within mscLat 1612.872 kb on - strand, within mscLat 1613.016 kb on + strand, within mscLat 1613.017 kb on - strand, within mscLat 1613.020 kb on - strand, within mscLat 1613.020 kb on - strand, within mscLat 1613.061 kb on + strand, within mscLat 1613.062 kb on - strand, within mscLat 1613.062 kb on - strand, within mscLat 1613.062 kb on - strand, within mscLat 1613.150 kb on + strandat 1613.162 kb on + strandat 1613.188 kb on + strandat 1613.189 kb on - strandat 1613.189 kb on - strandat 1613.189 kb on - strandat 1613.189 kb on - strandat 1613.189 kb on - strandat 1613.195 kb on - strandat 1613.286 kb on + strandat 1613.287 kb on - strandat 1613.290 kb on + strandat 1614.831 kb on - strand, within rsmBat 1614.831 kb on - strand, within rsmBat 1614.863 kb on - strand, within rsmBat 1614.863 kb on - strand, within rsmBat 1614.863 kb on - strand, within rsmBat 1614.966 kb on - strand, within rsmBat 1614.966 kb on - strand, within rsmBat 1614.966 kb on - strand, within rsmBat 1614.966 kb on - strand, within rsmBat 1614.966 kb on - strand, within rsmBat 1614.966 kb on - strand, within rsmBat 1615.018 kb on - strand, within rsmBat 1615.095 kb on - strand, within rsmBat 1615.095 kb on - strand, within rsmBat 1615.095 kb on - strand, within rsmBat 1615.095 kb on - strand, within rsmBat 1615.095 kb on - strand, within rsmBat 1615.108 kb on - strand, within rsmBat 1615.118 kb on - strand, within rsmBat 1615.351 kb on - strand, within rsmBat 1615.437 kb on - strand, within rsmBat 1615.443 kb on - strand, within rsmBat 1615.612 kb on - strand, within rsmBat 1615.615 kb on - strand, within rsmB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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1,612,359 - zntR MCAODC_08030 0.72 -1.1
1,612,410 - zntR MCAODC_08030 0.84 -1.0
1,612,410 - zntR MCAODC_08030 0.84 +0.8
1,612,419 + zntR MCAODC_08030 0.86 +0.1
1,612,420 - zntR MCAODC_08030 0.86 -0.9
1,612,498 + -1.4
1,612,498 + +0.5
1,612,498 + -0.7
1,612,498 + +0.5
1,612,498 + +1.1
1,612,499 - +0.3
1,612,516 + +0.7
1,612,551 + -0.3
1,612,551 + -0.5
1,612,551 + -0.3
1,612,551 + -0.4
1,612,552 - +0.3
1,612,554 + +0.5
1,612,554 + -0.4
1,612,580 + arfA MCAODC_08035 0.21 -1.0
1,612,581 - arfA MCAODC_08035 0.21 -0.1
1,612,590 - arfA MCAODC_08035 0.26 +2.1
1,612,592 + arfA MCAODC_08035 0.26 +0.9
1,612,592 + arfA MCAODC_08035 0.26 -0.5
1,612,592 + arfA MCAODC_08035 0.26 +0.1
1,612,592 + arfA MCAODC_08035 0.26 +0.9
1,612,592 + arfA MCAODC_08035 0.26 -1.7
1,612,592 + arfA MCAODC_08035 0.26 +3.2
1,612,629 + arfA MCAODC_08035 0.43 +1.1
1,612,648 + arfA MCAODC_08035 0.52 -0.2
1,612,648 + arfA MCAODC_08035 0.52 +2.1
1,612,649 - arfA MCAODC_08035 0.53 +0.7
1,612,649 - arfA MCAODC_08035 0.53 -0.5
1,612,649 - arfA MCAODC_08035 0.53 -1.8
1,612,707 - arfA MCAODC_08035 0.79 -1.4
1,612,750 + -1.8
1,612,751 - +1.5
1,612,751 - -0.5
1,612,751 - -0.3
1,612,800 + mscL MCAODC_08040 0.12 -1.3
1,612,800 + mscL MCAODC_08040 0.12 -0.7
1,612,801 - mscL MCAODC_08040 0.13 +0.1
1,612,801 - mscL MCAODC_08040 0.13 -0.0
1,612,801 - mscL MCAODC_08040 0.13 -0.3
1,612,801 - mscL MCAODC_08040 0.13 -1.8
1,612,811 + mscL MCAODC_08040 0.15 +2.1
1,612,811 + mscL MCAODC_08040 0.15 -0.3
1,612,812 - mscL MCAODC_08040 0.15 -0.8
1,612,812 - mscL MCAODC_08040 0.15 +0.8
1,612,866 + mscL MCAODC_08040 0.28 +1.6
1,612,866 + mscL MCAODC_08040 0.28 +0.1
1,612,867 - mscL MCAODC_08040 0.29 -0.1
1,612,871 + mscL MCAODC_08040 0.30 -0.5
1,612,872 - mscL MCAODC_08040 0.30 -0.9
1,613,016 + mscL MCAODC_08040 0.65 +1.3
1,613,017 - mscL MCAODC_08040 0.65 +1.3
1,613,020 - mscL MCAODC_08040 0.66 +0.1
1,613,020 - mscL MCAODC_08040 0.66 -1.1
1,613,061 + mscL MCAODC_08040 0.76 -0.3
1,613,062 - mscL MCAODC_08040 0.76 -0.8
1,613,062 - mscL MCAODC_08040 0.76 +0.8
1,613,062 - mscL MCAODC_08040 0.76 +0.6
1,613,150 + -0.2
1,613,162 + -3.0
1,613,188 + -0.3
1,613,189 - +0.4
1,613,189 - +0.8
1,613,189 - -0.6
1,613,189 - -0.5
1,613,189 - +0.7
1,613,195 - -0.4
1,613,286 + -0.8
1,613,287 - +0.1
1,613,290 + -1.7
1,614,831 - rsmB MCAODC_08050 0.11 -0.1
1,614,831 - rsmB MCAODC_08050 0.11 -1.7
1,614,863 - rsmB MCAODC_08050 0.14 +0.1
1,614,863 - rsmB MCAODC_08050 0.14 -0.8
1,614,863 - rsmB MCAODC_08050 0.14 -1.1
1,614,966 - rsmB MCAODC_08050 0.22 -0.9
1,614,966 - rsmB MCAODC_08050 0.22 +0.2
1,614,966 - rsmB MCAODC_08050 0.22 +0.3
1,614,966 - rsmB MCAODC_08050 0.22 -2.3
1,614,966 - rsmB MCAODC_08050 0.22 -1.5
1,614,966 - rsmB MCAODC_08050 0.22 +0.2
1,615,018 - rsmB MCAODC_08050 0.26 +0.5
1,615,095 - rsmB MCAODC_08050 0.32 -2.6
1,615,095 - rsmB MCAODC_08050 0.32 +1.3
1,615,095 - rsmB MCAODC_08050 0.32 -0.3
1,615,095 - rsmB MCAODC_08050 0.32 -1.3
1,615,095 - rsmB MCAODC_08050 0.32 -1.7
1,615,108 - rsmB MCAODC_08050 0.33 +1.4
1,615,118 - rsmB MCAODC_08050 0.33 -0.7
1,615,351 - rsmB MCAODC_08050 0.51 -0.2
1,615,437 - rsmB MCAODC_08050 0.58 +0.7
1,615,443 - rsmB MCAODC_08050 0.59 -0.3
1,615,612 - rsmB MCAODC_08050 0.72 -1.3
1,615,615 - rsmB MCAODC_08050 0.72 -0.6

Or see this region's nucleotide sequence