Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gpsA and cysE are separated by 79 nucleotides cysE and trmL are separated by 52 nucleotides trmL and lldD are separated by 185 nucleotides
MCAODC_06265: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 1,255,678 to 1,256,697
gpsA
MCAODC_06270: cysE - serine O-acetyltransferase, at 1,256,777 to 1,257,598
cysE
MCAODC_06275: trmL - tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine (34)-2'-O)-methyltransferase TrmL, at 1,257,651 to 1,258,124
trmL
MCAODC_06280: lldD - quinone-dependent L-lactate dehydrogenase, at 1,258,310 to 1,259,500
lldD
Position (kb)
1257
1258
1259 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1256.722 kb on - strand at 1256.754 kb on + strand at 1256.754 kb on + strand at 1256.755 kb on - strand at 1257.511 kb on + strand, within cysE at 1257.563 kb on - strand at 1257.563 kb on - strand at 1257.565 kb on + strand at 1257.565 kb on + strand at 1257.566 kb on - strand at 1257.571 kb on + strand at 1257.571 kb on + strand at 1257.571 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.626 kb on + strand at 1257.626 kb on + strand at 1257.626 kb on + strand at 1257.626 kb on + strand at 1257.627 kb on - strand at 1257.627 kb on - strand at 1257.627 kb on - strand at 1257.634 kb on + strand at 1257.635 kb on - strand at 1257.643 kb on + strand at 1257.643 kb on + strand at 1257.643 kb on + strand at 1257.643 kb on + strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.648 kb on + strand at 1257.649 kb on - strand at 1257.649 kb on - strand at 1257.649 kb on - strand at 1257.652 kb on + strand at 1257.652 kb on + strand at 1257.652 kb on + strand at 1257.652 kb on + strand at 1257.653 kb on - strand at 1257.653 kb on - strand at 1257.700 kb on + strand, within trmL at 1257.700 kb on + strand, within trmL at 1257.700 kb on + strand, within trmL at 1257.700 kb on + strand, within trmL at 1257.700 kb on + strand, within trmL at 1257.701 kb on - strand, within trmL at 1257.701 kb on - strand, within trmL at 1257.701 kb on - strand, within trmL at 1257.701 kb on - strand, within trmL at 1257.839 kb on - strand, within trmL at 1257.839 kb on - strand, within trmL at 1257.839 kb on - strand, within trmL at 1257.854 kb on - strand, within trmL at 1257.854 kb on - strand, within trmL at 1257.859 kb on - strand, within trmL at 1257.878 kb on + strand, within trmL at 1257.879 kb on - strand, within trmL at 1257.973 kb on + strand, within trmL at 1257.974 kb on - strand, within trmL at 1258.035 kb on - strand, within trmL at 1258.052 kb on + strand, within trmL at 1258.052 kb on + strand, within trmL at 1258.073 kb on + strand, within trmL at 1258.073 kb on + strand, within trmL at 1258.074 kb on - strand, within trmL at 1258.074 kb on - strand, within trmL at 1258.082 kb on + strand at 1258.083 kb on - strand at 1258.105 kb on + strand at 1258.105 kb on + strand at 1258.106 kb on - strand at 1258.106 kb on - strand at 1258.110 kb on + strand at 1258.110 kb on + strand at 1258.119 kb on + strand at 1258.120 kb on - strand at 1258.124 kb on + strand at 1258.125 kb on - strand at 1258.245 kb on + strand at 1258.245 kb on + strand at 1258.245 kb on + strand at 1258.245 kb on + strand at 1258.246 kb on - strand at 1258.246 kb on - strand at 1258.246 kb on - strand at 1258.246 kb on - strand at 1258.249 kb on + strand at 1258.249 kb on + strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.311 kb on + strand at 1258.391 kb on - strand at 1258.457 kb on + strand, within lldD at 1258.465 kb on + strand, within lldD at 1258.466 kb on - strand, within lldD at 1258.466 kb on - strand, within lldD at 1258.469 kb on + strand, within lldD at 1258.469 kb on + strand, within lldD at 1258.469 kb on + strand, within lldD at 1258.470 kb on - strand, within lldD at 1258.470 kb on - strand, within lldD at 1258.500 kb on + strand, within lldD at 1258.523 kb on + strand, within lldD at 1258.523 kb on + strand, within lldD at 1258.550 kb on - strand, within lldD at 1258.550 kb on - strand, within lldD at 1258.652 kb on + strand, within lldD at 1258.652 kb on + strand, within lldD at 1258.652 kb on + strand, within lldD at 1258.652 kb on + strand, within lldD at 1258.653 kb on - strand, within lldD at 1258.681 kb on + strand, within lldD at 1258.689 kb on - strand, within lldD at 1258.689 kb on - strand, within lldD at 1258.712 kb on + strand, within lldD at 1258.842 kb on + strand, within lldD at 1258.842 kb on + strand, within lldD at 1258.843 kb on - strand, within lldD at 1258.843 kb on - strand, within lldD at 1258.843 kb on - strand, within lldD at 1258.843 kb on - strand, within lldD at 1258.872 kb on + strand, within lldD at 1258.882 kb on + strand, within lldD at 1258.882 kb on + strand, within lldD at 1258.886 kb on - strand, within lldD at 1258.886 kb on - strand, within lldD at 1258.886 kb on - strand, within lldD at 1258.889 kb on + strand, within lldD at 1258.889 kb on + strand, within lldD at 1258.890 kb on - strand, within lldD at 1259.030 kb on - strand, within lldD at 1259.030 kb on - strand, within lldD at 1259.093 kb on - strand, within lldD at 1259.111 kb on + strand, within lldD at 1259.111 kb on + strand, within lldD at 1259.112 kb on - strand, within lldD at 1259.112 kb on - strand, within lldD at 1259.115 kb on + strand, within lldD at 1259.115 kb on + strand, within lldD at 1259.115 kb on + strand, within lldD at 1259.116 kb on - strand, within lldD at 1259.116 kb on - strand, within lldD at 1259.116 kb on - strand, within lldD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,256,722 - +2.1 1,256,754 + +0.2 1,256,754 + -0.6 1,256,755 - +1.7 1,257,511 + cysE MCAODC_06270 0.89 +1.8 1,257,563 - -1.7 1,257,563 - -0.1 1,257,565 + -0.3 1,257,565 + -1.7 1,257,566 - +0.1 1,257,571 + -1.9 1,257,571 + +0.1 1,257,571 + -2.0 1,257,596 + -2.6 1,257,596 + -1.3 1,257,596 + +0.6 1,257,596 + -1.1 1,257,596 + +0.1 1,257,596 + -0.0 1,257,596 + -0.7 1,257,596 + +0.5 1,257,596 + -0.1 1,257,596 + -0.3 1,257,597 - -1.5 1,257,597 - -0.1 1,257,597 - +0.9 1,257,597 - +1.7 1,257,597 - -1.2 1,257,597 - -1.2 1,257,597 - -0.2 1,257,626 + +0.1 1,257,626 + -1.5 1,257,626 + -0.8 1,257,626 + -1.8 1,257,627 - +0.9 1,257,627 - -0.5 1,257,627 - +0.2 1,257,634 + -0.4 1,257,635 - -0.7 1,257,643 + -0.0 1,257,643 + -0.0 1,257,643 + -0.1 1,257,643 + -0.5 1,257,644 - +0.1 1,257,644 - +0.7 1,257,644 - +0.2 1,257,644 - -1.7 1,257,644 - -0.1 1,257,644 - -0.5 1,257,648 + +0.7 1,257,649 - +1.5 1,257,649 - -0.4 1,257,649 - +0.2 1,257,652 + -0.7 1,257,652 + -0.2 1,257,652 + -1.4 1,257,652 + +0.7 1,257,653 - +0.5 1,257,653 - -0.6 1,257,700 + trmL MCAODC_06275 0.10 +3.1 1,257,700 + trmL MCAODC_06275 0.10 -0.7 1,257,700 + trmL MCAODC_06275 0.10 -0.9 1,257,700 + trmL MCAODC_06275 0.10 -0.3 1,257,700 + trmL MCAODC_06275 0.10 +0.2 1,257,701 - trmL MCAODC_06275 0.11 -2.3 1,257,701 - trmL MCAODC_06275 0.11 -0.9 1,257,701 - trmL MCAODC_06275 0.11 -1.0 1,257,701 - trmL MCAODC_06275 0.11 -0.2 1,257,839 - trmL MCAODC_06275 0.40 +0.7 1,257,839 - trmL MCAODC_06275 0.40 +1.1 1,257,839 - trmL MCAODC_06275 0.40 +0.1 1,257,854 - trmL MCAODC_06275 0.43 -0.4 1,257,854 - trmL MCAODC_06275 0.43 +0.2 1,257,859 - trmL MCAODC_06275 0.44 -1.3 1,257,878 + trmL MCAODC_06275 0.48 -0.1 1,257,879 - trmL MCAODC_06275 0.48 -0.5 1,257,973 + trmL MCAODC_06275 0.68 -1.5 1,257,974 - trmL MCAODC_06275 0.68 -0.9 1,258,035 - trmL MCAODC_06275 0.81 -0.1 1,258,052 + trmL MCAODC_06275 0.85 -2.0 1,258,052 + trmL MCAODC_06275 0.85 -0.6 1,258,073 + trmL MCAODC_06275 0.89 -0.6 1,258,073 + trmL MCAODC_06275 0.89 +1.1 1,258,074 - trmL MCAODC_06275 0.89 -2.3 1,258,074 - trmL MCAODC_06275 0.89 +0.6 1,258,082 + -1.1 1,258,083 - -0.3 1,258,105 + +0.6 1,258,105 + +0.1 1,258,106 - -1.9 1,258,106 - -1.0 1,258,110 + -1.4 1,258,110 + -0.3 1,258,119 + +0.3 1,258,120 - -0.2 1,258,124 + +0.2 1,258,125 - +0.7 1,258,245 + +0.5 1,258,245 + -3.0 1,258,245 + +0.1 1,258,245 + -0.1 1,258,246 - -0.2 1,258,246 - +0.2 1,258,246 - +0.7 1,258,246 - +0.6 1,258,249 + -1.2 1,258,249 + -0.7 1,258,250 - -0.6 1,258,250 - +1.6 1,258,250 - +1.5 1,258,250 - -1.3 1,258,250 - -0.3 1,258,250 - -0.3 1,258,311 + +1.1 1,258,391 - -1.1 1,258,457 + lldD MCAODC_06280 0.12 +0.4 1,258,465 + lldD MCAODC_06280 0.13 -0.3 1,258,466 - lldD MCAODC_06280 0.13 +0.8 1,258,466 - lldD MCAODC_06280 0.13 -1.5 1,258,469 + lldD MCAODC_06280 0.13 -0.3 1,258,469 + lldD MCAODC_06280 0.13 -0.6 1,258,469 + lldD MCAODC_06280 0.13 -0.2 1,258,470 - lldD MCAODC_06280 0.13 -0.6 1,258,470 - lldD MCAODC_06280 0.13 -0.8 1,258,500 + lldD MCAODC_06280 0.16 +0.5 1,258,523 + lldD MCAODC_06280 0.18 -0.7 1,258,523 + lldD MCAODC_06280 0.18 +1.1 1,258,550 - lldD MCAODC_06280 0.20 -0.9 1,258,550 - lldD MCAODC_06280 0.20 +1.2 1,258,652 + lldD MCAODC_06280 0.29 +0.6 1,258,652 + lldD MCAODC_06280 0.29 -0.9 1,258,652 + lldD MCAODC_06280 0.29 -2.9 1,258,652 + lldD MCAODC_06280 0.29 +0.6 1,258,653 - lldD MCAODC_06280 0.29 +0.8 1,258,681 + lldD MCAODC_06280 0.31 -1.0 1,258,689 - lldD MCAODC_06280 0.32 -0.7 1,258,689 - lldD MCAODC_06280 0.32 -0.1 1,258,712 + lldD MCAODC_06280 0.34 +0.1 1,258,842 + lldD MCAODC_06280 0.45 -0.8 1,258,842 + lldD MCAODC_06280 0.45 -0.5 1,258,843 - lldD MCAODC_06280 0.45 -1.2 1,258,843 - lldD MCAODC_06280 0.45 -1.3 1,258,843 - lldD MCAODC_06280 0.45 +0.7 1,258,843 - lldD MCAODC_06280 0.45 -1.6 1,258,872 + lldD MCAODC_06280 0.47 -0.1 1,258,882 + lldD MCAODC_06280 0.48 +2.1 1,258,882 + lldD MCAODC_06280 0.48 -0.5 1,258,886 - lldD MCAODC_06280 0.48 +0.3 1,258,886 - lldD MCAODC_06280 0.48 -1.0 1,258,886 - lldD MCAODC_06280 0.48 -1.0 1,258,889 + lldD MCAODC_06280 0.49 -1.6 1,258,889 + lldD MCAODC_06280 0.49 +1.2 1,258,890 - lldD MCAODC_06280 0.49 -1.5 1,259,030 - lldD MCAODC_06280 0.60 -0.1 1,259,030 - lldD MCAODC_06280 0.60 -0.3 1,259,093 - lldD MCAODC_06280 0.66 -1.3 1,259,111 + lldD MCAODC_06280 0.67 -0.1 1,259,111 + lldD MCAODC_06280 0.67 +1.1 1,259,112 - lldD MCAODC_06280 0.67 +0.5 1,259,112 - lldD MCAODC_06280 0.67 -0.1 1,259,115 + lldD MCAODC_06280 0.68 -1.2 1,259,115 + lldD MCAODC_06280 0.68 -2.1 1,259,115 + lldD MCAODC_06280 0.68 -0.5 1,259,116 - lldD MCAODC_06280 0.68 +1.6 1,259,116 - lldD MCAODC_06280 0.68 -0.1 1,259,116 - lldD MCAODC_06280 0.68 -1.7
Or see this region's nucleotide sequence