Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvG and MCAODC_05165 are separated by 346 nucleotides MCAODC_05165 and yifB are separated by 154 nucleotides
MCAODC_05160: ilvG - acetolactate synthase 2 catalytic subunit, at 1,040,531 to 1,042,177
ilvG
MCAODC_05165: MCAODC_05165 - hypothetical protein, at 1,042,524 to 1,042,613
_05165
MCAODC_05170: yifB - YifB family Mg chelatase-like AAA ATPase, at 1,042,768 to 1,044,288
yifB
Position (kb)
1042
1043 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1041.573 kb on + strand, within ilvG at 1041.573 kb on + strand, within ilvG at 1041.573 kb on + strand, within ilvG at 1041.573 kb on + strand, within ilvG at 1041.573 kb on + strand, within ilvG at 1041.573 kb on + strand, within ilvG at 1041.573 kb on + strand, within ilvG at 1041.573 kb on + strand, within ilvG at 1041.573 kb on + strand, within ilvG at 1041.574 kb on - strand, within ilvG at 1041.574 kb on - strand, within ilvG at 1041.574 kb on - strand, within ilvG at 1041.574 kb on - strand, within ilvG at 1041.574 kb on - strand, within ilvG at 1041.698 kb on - strand, within ilvG at 1041.698 kb on - strand, within ilvG at 1041.766 kb on + strand, within ilvG at 1041.815 kb on - strand, within ilvG at 1041.910 kb on + strand, within ilvG at 1041.911 kb on - strand, within ilvG at 1041.917 kb on + strand, within ilvG at 1041.976 kb on + strand, within ilvG at 1041.976 kb on + strand, within ilvG at 1041.976 kb on + strand, within ilvG at 1041.976 kb on + strand, within ilvG at 1041.976 kb on + strand, within ilvG at 1041.976 kb on + strand, within ilvG at 1041.976 kb on + strand, within ilvG at 1041.980 kb on + strand, within ilvG at 1041.980 kb on + strand, within ilvG at 1041.980 kb on + strand, within ilvG at 1041.980 kb on + strand, within ilvG at 1041.981 kb on - strand, within ilvG at 1041.981 kb on - strand, within ilvG at 1042.074 kb on + strand at 1042.074 kb on + strand at 1042.075 kb on - strand at 1042.075 kb on - strand at 1042.075 kb on - strand at 1042.075 kb on - strand at 1042.075 kb on - strand at 1042.075 kb on - strand at 1042.086 kb on + strand at 1042.096 kb on + strand at 1042.096 kb on + strand at 1042.097 kb on - strand at 1042.154 kb on + strand at 1042.154 kb on + strand at 1042.155 kb on - strand at 1042.155 kb on - strand at 1042.155 kb on - strand at 1042.155 kb on - strand at 1042.155 kb on - strand at 1042.155 kb on - strand at 1042.177 kb on + strand at 1042.178 kb on - strand at 1042.178 kb on - strand at 1042.178 kb on - strand at 1042.181 kb on + strand at 1042.182 kb on - strand at 1042.296 kb on + strand at 1042.296 kb on + strand at 1042.297 kb on - strand at 1042.297 kb on - strand at 1042.318 kb on + strand at 1042.318 kb on + strand at 1042.361 kb on + strand at 1042.402 kb on - strand at 1042.402 kb on - strand at 1042.465 kb on - strand at 1042.477 kb on + strand at 1042.487 kb on + strand at 1042.606 kb on + strand at 1042.607 kb on - strand at 1042.682 kb on - strand at 1042.737 kb on + strand at 1042.737 kb on + strand at 1042.738 kb on - strand at 1042.738 kb on - strand at 1042.746 kb on - strand at 1042.767 kb on + strand at 1042.767 kb on + strand at 1042.767 kb on + strand at 1042.767 kb on + strand at 1042.767 kb on + strand at 1042.767 kb on + strand at 1042.767 kb on + strand at 1042.767 kb on + strand at 1042.768 kb on - strand at 1042.768 kb on - strand at 1042.768 kb on - strand at 1042.768 kb on - strand at 1042.789 kb on - strand at 1042.835 kb on + strand at 1042.835 kb on + strand at 1042.835 kb on + strand at 1042.836 kb on - strand at 1042.836 kb on - strand at 1042.836 kb on - strand at 1042.836 kb on - strand at 1042.839 kb on + strand at 1042.839 kb on + strand at 1042.839 kb on + strand at 1042.839 kb on + strand at 1042.839 kb on + strand at 1042.839 kb on + strand at 1042.839 kb on + strand at 1042.840 kb on - strand at 1042.840 kb on - strand at 1042.840 kb on - strand at 1042.840 kb on - strand at 1042.840 kb on - strand at 1042.840 kb on - strand at 1042.840 kb on - strand at 1042.840 kb on - strand at 1042.840 kb on - strand at 1042.840 kb on - strand at 1042.862 kb on + strand at 1042.892 kb on + strand at 1042.926 kb on + strand, within yifB at 1042.926 kb on + strand, within yifB at 1042.927 kb on - strand, within yifB at 1042.927 kb on - strand, within yifB at 1042.939 kb on + strand, within yifB at 1042.969 kb on - strand, within yifB at 1042.988 kb on + strand, within yifB at 1043.015 kb on + strand, within yifB at 1043.015 kb on + strand, within yifB at 1043.015 kb on + strand, within yifB at 1043.016 kb on - strand, within yifB at 1043.016 kb on - strand, within yifB at 1043.016 kb on - strand, within yifB at 1043.019 kb on + strand, within yifB at 1043.020 kb on - strand, within yifB at 1043.055 kb on + strand, within yifB at 1043.070 kb on - strand, within yifB at 1043.070 kb on - strand, within yifB at 1043.070 kb on - strand, within yifB at 1043.070 kb on - strand, within yifB at 1043.078 kb on - strand, within yifB at 1043.085 kb on - strand, within yifB at 1043.085 kb on - strand, within yifB at 1043.104 kb on - strand, within yifB at 1043.104 kb on - strand, within yifB at 1043.104 kb on - strand, within yifB at 1043.154 kb on + strand, within yifB at 1043.154 kb on + strand, within yifB at 1043.155 kb on - strand, within yifB at 1043.157 kb on + strand, within yifB at 1043.158 kb on - strand, within yifB at 1043.158 kb on - strand, within yifB at 1043.191 kb on - strand, within yifB at 1043.191 kb on - strand, within yifB at 1043.210 kb on + strand, within yifB at 1043.211 kb on - strand, within yifB at 1043.214 kb on + strand, within yifB at 1043.274 kb on + strand, within yifB at 1043.274 kb on + strand, within yifB at 1043.274 kb on + strand, within yifB at 1043.274 kb on + strand, within yifB at 1043.275 kb on - strand, within yifB at 1043.275 kb on - strand, within yifB at 1043.324 kb on + strand, within yifB at 1043.324 kb on + strand, within yifB at 1043.325 kb on - strand, within yifB at 1043.433 kb on + strand, within yifB at 1043.434 kb on - strand, within yifB at 1043.434 kb on - strand, within yifB at 1043.454 kb on + strand, within yifB at 1043.454 kb on + strand, within yifB at 1043.454 kb on + strand, within yifB at 1043.454 kb on + strand, within yifB at 1043.455 kb on - strand, within yifB at 1043.455 kb on - strand, within yifB at 1043.458 kb on - strand, within yifB at 1043.458 kb on - strand, within yifB at 1043.477 kb on + strand, within yifB at 1043.478 kb on - strand, within yifB at 1043.478 kb on - strand, within yifB at 1043.478 kb on - strand, within yifB at 1043.513 kb on + strand, within yifB at 1043.514 kb on - strand, within yifB at 1043.522 kb on + strand, within yifB at 1043.537 kb on + strand, within yifB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,041,573 + ilvG MCAODC_05160 0.63 -0.1 1,041,573 + ilvG MCAODC_05160 0.63 +0.7 1,041,573 + ilvG MCAODC_05160 0.63 -0.7 1,041,573 + ilvG MCAODC_05160 0.63 -0.3 1,041,573 + ilvG MCAODC_05160 0.63 -0.0 1,041,573 + ilvG MCAODC_05160 0.63 +1.8 1,041,573 + ilvG MCAODC_05160 0.63 -1.1 1,041,573 + ilvG MCAODC_05160 0.63 +0.1 1,041,573 + ilvG MCAODC_05160 0.63 -0.0 1,041,574 - ilvG MCAODC_05160 0.63 +0.1 1,041,574 - ilvG MCAODC_05160 0.63 -0.2 1,041,574 - ilvG MCAODC_05160 0.63 +0.5 1,041,574 - ilvG MCAODC_05160 0.63 +0.5 1,041,574 - ilvG MCAODC_05160 0.63 +1.1 1,041,698 - ilvG MCAODC_05160 0.71 +1.3 1,041,698 - ilvG MCAODC_05160 0.71 -1.2 1,041,766 + ilvG MCAODC_05160 0.75 +1.5 1,041,815 - ilvG MCAODC_05160 0.78 +0.7 1,041,910 + ilvG MCAODC_05160 0.84 -0.5 1,041,911 - ilvG MCAODC_05160 0.84 -0.6 1,041,917 + ilvG MCAODC_05160 0.84 -0.2 1,041,976 + ilvG MCAODC_05160 0.88 -0.7 1,041,976 + ilvG MCAODC_05160 0.88 -0.6 1,041,976 + ilvG MCAODC_05160 0.88 -1.5 1,041,976 + ilvG MCAODC_05160 0.88 -1.8 1,041,976 + ilvG MCAODC_05160 0.88 -0.5 1,041,976 + ilvG MCAODC_05160 0.88 -1.1 1,041,976 + ilvG MCAODC_05160 0.88 -0.9 1,041,980 + ilvG MCAODC_05160 0.88 +0.5 1,041,980 + ilvG MCAODC_05160 0.88 -0.1 1,041,980 + ilvG MCAODC_05160 0.88 -0.4 1,041,980 + ilvG MCAODC_05160 0.88 -0.5 1,041,981 - ilvG MCAODC_05160 0.88 +0.7 1,041,981 - ilvG MCAODC_05160 0.88 +2.1 1,042,074 + +0.1 1,042,074 + +1.7 1,042,075 - -0.9 1,042,075 - +2.2 1,042,075 - +0.3 1,042,075 - -0.5 1,042,075 - -0.8 1,042,075 - +0.1 1,042,086 + -0.9 1,042,096 + -2.8 1,042,096 + -0.5 1,042,097 - +0.1 1,042,154 + -0.2 1,042,154 + -1.5 1,042,155 - -1.0 1,042,155 - +3.1 1,042,155 - -1.6 1,042,155 - -0.6 1,042,155 - +1.7 1,042,155 - -0.2 1,042,177 + -0.5 1,042,178 - -1.0 1,042,178 - -0.6 1,042,178 - -0.2 1,042,181 + -0.1 1,042,182 - -0.5 1,042,296 + +1.8 1,042,296 + -0.7 1,042,297 - +1.9 1,042,297 - +0.9 1,042,318 + +1.3 1,042,318 + +0.0 1,042,361 + -0.2 1,042,402 - +0.2 1,042,402 - -2.5 1,042,465 - +0.3 1,042,477 + -0.4 1,042,487 + +0.3 1,042,606 + +0.7 1,042,607 - -1.5 1,042,682 - +0.6 1,042,737 + +1.1 1,042,737 + -0.6 1,042,738 - -0.7 1,042,738 - -0.6 1,042,746 - +1.0 1,042,767 + +0.7 1,042,767 + +2.4 1,042,767 + +2.1 1,042,767 + +0.9 1,042,767 + +2.1 1,042,767 + -0.5 1,042,767 + -0.3 1,042,767 + +2.1 1,042,768 - +0.1 1,042,768 - +0.1 1,042,768 - +2.1 1,042,768 - +1.1 1,042,789 - +1.5 1,042,835 + -0.9 1,042,835 + +0.2 1,042,835 + -3.3 1,042,836 - -0.7 1,042,836 - +0.4 1,042,836 - -2.0 1,042,836 - -1.4 1,042,839 + +1.3 1,042,839 + +0.3 1,042,839 + -0.4 1,042,839 + -2.0 1,042,839 + +0.9 1,042,839 + -1.1 1,042,839 + +0.2 1,042,840 - +0.3 1,042,840 - -0.7 1,042,840 - -1.3 1,042,840 - +0.5 1,042,840 - -1.7 1,042,840 - -0.9 1,042,840 - +0.3 1,042,840 - -0.0 1,042,840 - -1.6 1,042,840 - -0.7 1,042,862 + +0.5 1,042,892 + -0.2 1,042,926 + yifB MCAODC_05170 0.10 -0.7 1,042,926 + yifB MCAODC_05170 0.10 +0.7 1,042,927 - yifB MCAODC_05170 0.10 -0.2 1,042,927 - yifB MCAODC_05170 0.10 -0.6 1,042,939 + yifB MCAODC_05170 0.11 -0.5 1,042,969 - yifB MCAODC_05170 0.13 -0.9 1,042,988 + yifB MCAODC_05170 0.14 +0.9 1,043,015 + yifB MCAODC_05170 0.16 +1.2 1,043,015 + yifB MCAODC_05170 0.16 +1.4 1,043,015 + yifB MCAODC_05170 0.16 -0.9 1,043,016 - yifB MCAODC_05170 0.16 -0.8 1,043,016 - yifB MCAODC_05170 0.16 -1.2 1,043,016 - yifB MCAODC_05170 0.16 +0.6 1,043,019 + yifB MCAODC_05170 0.17 +1.2 1,043,020 - yifB MCAODC_05170 0.17 +0.8 1,043,055 + yifB MCAODC_05170 0.19 +1.2 1,043,070 - yifB MCAODC_05170 0.20 +0.4 1,043,070 - yifB MCAODC_05170 0.20 +1.0 1,043,070 - yifB MCAODC_05170 0.20 +0.3 1,043,070 - yifB MCAODC_05170 0.20 +0.1 1,043,078 - yifB MCAODC_05170 0.20 +0.7 1,043,085 - yifB MCAODC_05170 0.21 -0.7 1,043,085 - yifB MCAODC_05170 0.21 -1.2 1,043,104 - yifB MCAODC_05170 0.22 +0.0 1,043,104 - yifB MCAODC_05170 0.22 +0.8 1,043,104 - yifB MCAODC_05170 0.22 -0.3 1,043,154 + yifB MCAODC_05170 0.25 -2.3 1,043,154 + yifB MCAODC_05170 0.25 -0.0 1,043,155 - yifB MCAODC_05170 0.25 +0.8 1,043,157 + yifB MCAODC_05170 0.26 -0.7 1,043,158 - yifB MCAODC_05170 0.26 +0.5 1,043,158 - yifB MCAODC_05170 0.26 -0.5 1,043,191 - yifB MCAODC_05170 0.28 +0.1 1,043,191 - yifB MCAODC_05170 0.28 -3.0 1,043,210 + yifB MCAODC_05170 0.29 +2.3 1,043,211 - yifB MCAODC_05170 0.29 +0.4 1,043,214 + yifB MCAODC_05170 0.29 +0.6 1,043,274 + yifB MCAODC_05170 0.33 +0.1 1,043,274 + yifB MCAODC_05170 0.33 -0.5 1,043,274 + yifB MCAODC_05170 0.33 -1.4 1,043,274 + yifB MCAODC_05170 0.33 -0.4 1,043,275 - yifB MCAODC_05170 0.33 -0.4 1,043,275 - yifB MCAODC_05170 0.33 -0.2 1,043,324 + yifB MCAODC_05170 0.37 +0.1 1,043,324 + yifB MCAODC_05170 0.37 +1.2 1,043,325 - yifB MCAODC_05170 0.37 +2.1 1,043,433 + yifB MCAODC_05170 0.44 +0.3 1,043,434 - yifB MCAODC_05170 0.44 -0.7 1,043,434 - yifB MCAODC_05170 0.44 -0.2 1,043,454 + yifB MCAODC_05170 0.45 +1.2 1,043,454 + yifB MCAODC_05170 0.45 -1.1 1,043,454 + yifB MCAODC_05170 0.45 +0.7 1,043,454 + yifB MCAODC_05170 0.45 -1.8 1,043,455 - yifB MCAODC_05170 0.45 -1.1 1,043,455 - yifB MCAODC_05170 0.45 -0.6 1,043,458 - yifB MCAODC_05170 0.45 +0.1 1,043,458 - yifB MCAODC_05170 0.45 -0.5 1,043,477 + yifB MCAODC_05170 0.47 -0.5 1,043,478 - yifB MCAODC_05170 0.47 -1.2 1,043,478 - yifB MCAODC_05170 0.47 -2.2 1,043,478 - yifB MCAODC_05170 0.47 +1.8 1,043,513 + yifB MCAODC_05170 0.49 +0.2 1,043,514 - yifB MCAODC_05170 0.49 +0.8 1,043,522 + yifB MCAODC_05170 0.50 +1.6 1,043,537 + yifB MCAODC_05170 0.51 +0.9
Or see this region's nucleotide sequence