Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nanM and nanS are separated by 64 nucleotides nanS and MCAODC_02150 are separated by 295 nucleotides MCAODC_02150 and MCAODC_02155 are separated by 101 nucleotides MCAODC_02155 and MCAODC_02160 are separated by 159 nucleotides
MCAODC_02140: nanM - N-acetylneuraminate epimerase, at 421,944 to 423,050
nanM
MCAODC_02145: nanS - putative 9-O-acetyl-N-acetylneuraminic acid deacetylase, at 423,115 to 424,095
nanS
MCAODC_02150: MCAODC_02150 - Transposase, IS4 family protein, at 424,391 to 424,636
_02150
MCAODC_02155: MCAODC_02155 - Transposase, at 424,738 to 424,824
_02155
MCAODC_02160: MCAODC_02160 - Orf, at 424,984 to 425,022
_02160
Position (kb) 423
424
425 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 422.144 kb on - strand, within nanM at 422.189 kb on + strand, within nanM at 422.190 kb on - strand, within nanM at 422.190 kb on - strand, within nanM at 422.190 kb on - strand, within nanM at 422.205 kb on + strand, within nanM at 422.206 kb on - strand, within nanM at 422.263 kb on + strand, within nanM at 422.271 kb on + strand, within nanM at 422.272 kb on - strand, within nanM at 422.272 kb on - strand, within nanM at 422.365 kb on - strand, within nanM at 422.365 kb on - strand, within nanM at 422.466 kb on + strand, within nanM at 422.615 kb on + strand, within nanM at 422.826 kb on + strand, within nanM at 422.864 kb on + strand, within nanM at 422.933 kb on - strand, within nanM at 422.948 kb on + strand at 422.957 kb on - strand at 423.125 kb on + strand at 423.145 kb on + strand at 423.146 kb on - strand at 423.172 kb on - strand at 423.336 kb on + strand, within nanS at 423.337 kb on - strand, within nanS at 423.346 kb on + strand, within nanS at 423.346 kb on + strand, within nanS at 423.346 kb on + strand, within nanS at 423.408 kb on + strand, within nanS at 423.409 kb on - strand, within nanS at 423.409 kb on - strand, within nanS at 423.442 kb on - strand, within nanS at 423.442 kb on - strand, within nanS at 423.511 kb on + strand, within nanS at 423.511 kb on + strand, within nanS at 423.511 kb on + strand, within nanS at 423.512 kb on - strand, within nanS at 423.512 kb on - strand, within nanS at 423.571 kb on + strand, within nanS at 423.581 kb on + strand, within nanS at 423.638 kb on + strand, within nanS at 423.639 kb on - strand, within nanS at 423.665 kb on + strand, within nanS at 423.666 kb on - strand, within nanS at 423.666 kb on - strand, within nanS at 423.666 kb on - strand, within nanS at 423.680 kb on - strand, within nanS at 423.702 kb on + strand, within nanS at 423.703 kb on - strand, within nanS at 423.703 kb on - strand, within nanS at 423.709 kb on - strand, within nanS at 423.734 kb on + strand, within nanS at 423.742 kb on + strand, within nanS at 423.742 kb on + strand, within nanS at 423.743 kb on - strand, within nanS at 423.757 kb on - strand, within nanS at 423.757 kb on - strand, within nanS at 423.760 kb on - strand, within nanS at 423.765 kb on + strand, within nanS at 423.766 kb on - strand, within nanS at 423.803 kb on - strand, within nanS at 423.829 kb on + strand, within nanS at 423.837 kb on + strand, within nanS at 423.858 kb on + strand, within nanS at 423.870 kb on + strand, within nanS at 423.870 kb on + strand, within nanS at 423.870 kb on + strand, within nanS at 423.948 kb on - strand, within nanS at 423.948 kb on - strand, within nanS at 423.962 kb on - strand, within nanS at 423.962 kb on - strand, within nanS at 424.104 kb on + strand at 424.136 kb on + strand at 424.136 kb on + strand at 424.136 kb on + strand at 424.158 kb on + strand at 424.158 kb on + strand at 424.158 kb on + strand at 424.159 kb on - strand at 424.159 kb on - strand at 424.191 kb on + strand at 424.192 kb on - strand at 424.194 kb on - strand at 424.232 kb on + strand at 424.541 kb on + strand, within MCAODC_02150 at 424.544 kb on + strand, within MCAODC_02150 at 424.544 kb on + strand, within MCAODC_02150 at 424.608 kb on + strand, within MCAODC_02150 at 424.631 kb on + strand at 424.631 kb on + strand at 424.631 kb on + strand at 424.631 kb on + strand at 424.708 kb on - strand at 424.708 kb on - strand at 424.864 kb on + strand at 424.907 kb on + strand at 424.908 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 422,144 - nanM MCAODC_02140 0.18 +3.0 422,189 + nanM MCAODC_02140 0.22 -0.8 422,190 - nanM MCAODC_02140 0.22 -1.0 422,190 - nanM MCAODC_02140 0.22 +1.0 422,190 - nanM MCAODC_02140 0.22 +2.0 422,205 + nanM MCAODC_02140 0.24 +0.3 422,206 - nanM MCAODC_02140 0.24 +2.0 422,263 + nanM MCAODC_02140 0.29 +0.2 422,271 + nanM MCAODC_02140 0.30 -1.1 422,272 - nanM MCAODC_02140 0.30 +0.4 422,272 - nanM MCAODC_02140 0.30 +0.4 422,365 - nanM MCAODC_02140 0.38 +1.0 422,365 - nanM MCAODC_02140 0.38 -0.6 422,466 + nanM MCAODC_02140 0.47 +2.5 422,615 + nanM MCAODC_02140 0.61 +0.0 422,826 + nanM MCAODC_02140 0.80 -0.4 422,864 + nanM MCAODC_02140 0.83 +0.4 422,933 - nanM MCAODC_02140 0.89 +0.4 422,948 + +1.3 422,957 - -0.4 423,125 + -2.1 423,145 + -0.2 423,146 - -0.7 423,172 - -2.2 423,336 + nanS MCAODC_02145 0.23 -1.4 423,337 - nanS MCAODC_02145 0.23 +0.4 423,346 + nanS MCAODC_02145 0.24 +1.1 423,346 + nanS MCAODC_02145 0.24 -0.3 423,346 + nanS MCAODC_02145 0.24 +0.5 423,408 + nanS MCAODC_02145 0.30 +2.4 423,409 - nanS MCAODC_02145 0.30 +0.2 423,409 - nanS MCAODC_02145 0.30 -0.6 423,442 - nanS MCAODC_02145 0.33 +2.3 423,442 - nanS MCAODC_02145 0.33 -0.2 423,511 + nanS MCAODC_02145 0.40 -2.6 423,511 + nanS MCAODC_02145 0.40 +0.0 423,511 + nanS MCAODC_02145 0.40 -0.8 423,512 - nanS MCAODC_02145 0.40 +1.0 423,512 - nanS MCAODC_02145 0.40 +0.5 423,571 + nanS MCAODC_02145 0.46 -0.8 423,581 + nanS MCAODC_02145 0.48 -0.1 423,638 + nanS MCAODC_02145 0.53 +1.3 423,639 - nanS MCAODC_02145 0.53 +0.0 423,665 + nanS MCAODC_02145 0.56 -1.0 423,666 - nanS MCAODC_02145 0.56 -0.7 423,666 - nanS MCAODC_02145 0.56 -1.9 423,666 - nanS MCAODC_02145 0.56 +2.0 423,680 - nanS MCAODC_02145 0.58 +1.7 423,702 + nanS MCAODC_02145 0.60 +0.3 423,703 - nanS MCAODC_02145 0.60 +0.2 423,703 - nanS MCAODC_02145 0.60 +0.0 423,709 - nanS MCAODC_02145 0.61 -0.6 423,734 + nanS MCAODC_02145 0.63 +0.0 423,742 + nanS MCAODC_02145 0.64 +1.3 423,742 + nanS MCAODC_02145 0.64 -0.1 423,743 - nanS MCAODC_02145 0.64 -1.1 423,757 - nanS MCAODC_02145 0.65 -1.3 423,757 - nanS MCAODC_02145 0.65 +0.2 423,760 - nanS MCAODC_02145 0.66 +1.4 423,765 + nanS MCAODC_02145 0.66 +0.4 423,766 - nanS MCAODC_02145 0.66 +1.0 423,803 - nanS MCAODC_02145 0.70 +1.2 423,829 + nanS MCAODC_02145 0.73 -0.3 423,837 + nanS MCAODC_02145 0.74 -1.1 423,858 + nanS MCAODC_02145 0.76 +0.8 423,870 + nanS MCAODC_02145 0.77 -0.8 423,870 + nanS MCAODC_02145 0.77 +0.2 423,870 + nanS MCAODC_02145 0.77 -0.0 423,948 - nanS MCAODC_02145 0.85 -1.1 423,948 - nanS MCAODC_02145 0.85 -0.1 423,962 - nanS MCAODC_02145 0.86 +0.2 423,962 - nanS MCAODC_02145 0.86 -1.1 424,104 + +2.2 424,136 + +0.4 424,136 + +1.0 424,136 + +1.0 424,158 + -0.6 424,158 + -1.8 424,158 + +0.5 424,159 - -0.5 424,159 - +0.9 424,191 + -0.3 424,192 - -0.2 424,194 - +1.0 424,232 + +1.0 424,541 + MCAODC_02150 0.61 -1.3 424,544 + MCAODC_02150 0.62 -1.8 424,544 + MCAODC_02150 0.62 -0.3 424,608 + MCAODC_02150 0.88 +0.9 424,631 + -1.3 424,631 + -0.3 424,631 + -0.9 424,631 + +1.4 424,708 - -0.7 424,708 - +0.4 424,864 + +1.3 424,907 + +0.8 424,908 - +0.3
Or see this region's nucleotide sequence