Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrC and thrB are separated by 0 nucleotides thrB and thrA are separated by 1 nucleotides
MCAODC_01615: thrC - threonine synthase, at 312,153 to 313,439
thrC
MCAODC_01620: thrB - homoserine kinase, at 313,440 to 314,372
thrB
MCAODC_01625: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 314,374 to 316,836
thrA
Position (kb) 313
314
315 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 312.457 kb on + strand, within thrC at 312.632 kb on + strand, within thrC at 312.632 kb on + strand, within thrC at 312.632 kb on + strand, within thrC at 312.798 kb on - strand, within thrC at 312.806 kb on + strand, within thrC at 312.807 kb on - strand, within thrC at 312.926 kb on + strand, within thrC at 312.927 kb on - strand, within thrC at 312.927 kb on - strand, within thrC at 313.003 kb on + strand, within thrC at 313.004 kb on - strand, within thrC at 313.079 kb on + strand, within thrC at 313.121 kb on + strand, within thrC at 313.121 kb on + strand, within thrC at 313.121 kb on + strand, within thrC at 313.151 kb on - strand, within thrC at 313.253 kb on + strand, within thrC at 313.421 kb on + strand at 313.422 kb on - strand at 313.422 kb on - strand at 313.422 kb on - strand at 313.429 kb on + strand at 313.441 kb on + strand at 313.452 kb on + strand at 313.484 kb on + strand at 313.484 kb on + strand at 313.484 kb on + strand at 313.513 kb on + strand at 313.513 kb on + strand at 313.513 kb on + strand at 313.514 kb on - strand at 313.520 kb on + strand at 313.599 kb on - strand, within thrB at 313.685 kb on + strand, within thrB at 313.729 kb on + strand, within thrB at 313.730 kb on - strand, within thrB at 313.832 kb on + strand, within thrB at 313.922 kb on + strand, within thrB at 313.922 kb on + strand, within thrB at 313.923 kb on - strand, within thrB at 314.018 kb on + strand, within thrB at 314.085 kb on + strand, within thrB at 314.176 kb on + strand, within thrB at 314.268 kb on + strand, within thrB at 314.268 kb on + strand, within thrB at 314.268 kb on + strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.336 kb on + strand at 314.337 kb on - strand at 314.384 kb on - strand at 314.384 kb on - strand at 314.401 kb on - strand at 314.401 kb on - strand at 314.401 kb on - strand at 314.404 kb on + strand at 314.404 kb on + strand at 314.405 kb on - strand at 314.405 kb on - strand at 314.405 kb on - strand at 314.484 kb on - strand at 314.486 kb on + strand at 314.486 kb on + strand at 314.487 kb on - strand at 314.487 kb on - strand at 314.495 kb on + strand at 314.496 kb on - strand at 314.592 kb on + strand at 314.603 kb on + strand at 314.604 kb on - strand at 314.604 kb on - strand at 314.604 kb on - strand at 314.683 kb on - strand, within thrA at 314.683 kb on - strand, within thrA at 314.786 kb on - strand, within thrA at 314.788 kb on + strand, within thrA at 314.788 kb on + strand, within thrA at 314.789 kb on - strand, within thrA at 314.793 kb on + strand, within thrA at 314.794 kb on - strand, within thrA at 314.794 kb on - strand, within thrA at 314.794 kb on - strand, within thrA at 314.800 kb on + strand, within thrA at 314.801 kb on - strand, within thrA at 314.801 kb on - strand, within thrA at 314.801 kb on - strand, within thrA at 314.844 kb on - strand, within thrA at 314.910 kb on + strand, within thrA at 314.911 kb on - strand, within thrA at 314.911 kb on - strand, within thrA at 314.911 kb on - strand, within thrA at 314.912 kb on + strand, within thrA at 314.912 kb on + strand, within thrA at 314.912 kb on + strand, within thrA at 314.912 kb on + strand, within thrA at 314.912 kb on + strand, within thrA at 314.912 kb on + strand, within thrA at 314.913 kb on - strand, within thrA at 314.913 kb on - strand, within thrA at 315.024 kb on - strand, within thrA at 315.024 kb on - strand, within thrA at 315.071 kb on + strand, within thrA at 315.072 kb on - strand, within thrA at 315.072 kb on - strand, within thrA at 315.075 kb on - strand, within thrA at 315.146 kb on + strand, within thrA at 315.146 kb on + strand, within thrA at 315.206 kb on + strand, within thrA at 315.240 kb on + strand, within thrA at 315.290 kb on - strand, within thrA at 315.324 kb on + strand, within thrA at 315.343 kb on + strand, within thrA at 315.343 kb on + strand, within thrA at 315.343 kb on + strand, within thrA at 315.343 kb on + strand, within thrA at 315.343 kb on + strand, within thrA at 315.343 kb on + strand, within thrA at 315.343 kb on + strand, within thrA at 315.344 kb on - strand, within thrA at 315.344 kb on - strand, within thrA at 315.344 kb on - strand, within thrA at 315.351 kb on + strand, within thrA at 315.352 kb on - strand, within thrA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 312,457 + thrC MCAODC_01615 0.24 -0.7 312,632 + thrC MCAODC_01615 0.37 -0.8 312,632 + thrC MCAODC_01615 0.37 -0.6 312,632 + thrC MCAODC_01615 0.37 -1.9 312,798 - thrC MCAODC_01615 0.50 -1.6 312,806 + thrC MCAODC_01615 0.51 +1.0 312,807 - thrC MCAODC_01615 0.51 +1.4 312,926 + thrC MCAODC_01615 0.60 +2.2 312,927 - thrC MCAODC_01615 0.60 -0.3 312,927 - thrC MCAODC_01615 0.60 -0.7 313,003 + thrC MCAODC_01615 0.66 -1.1 313,004 - thrC MCAODC_01615 0.66 -0.4 313,079 + thrC MCAODC_01615 0.72 -0.1 313,121 + thrC MCAODC_01615 0.75 +1.8 313,121 + thrC MCAODC_01615 0.75 -0.0 313,121 + thrC MCAODC_01615 0.75 -0.6 313,151 - thrC MCAODC_01615 0.78 +0.2 313,253 + thrC MCAODC_01615 0.85 -0.8 313,421 + +0.3 313,422 - +0.5 313,422 - -0.8 313,422 - -0.1 313,429 + -0.0 313,441 + -0.3 313,452 + -1.8 313,484 + -1.5 313,484 + -0.6 313,484 + -2.9 313,513 + -0.3 313,513 + -0.2 313,513 + +2.0 313,514 - -2.1 313,520 + -0.9 313,599 - thrB MCAODC_01620 0.17 +0.2 313,685 + thrB MCAODC_01620 0.26 -0.9 313,729 + thrB MCAODC_01620 0.31 +0.1 313,730 - thrB MCAODC_01620 0.31 -0.3 313,832 + thrB MCAODC_01620 0.42 +5.0 313,922 + thrB MCAODC_01620 0.52 -1.2 313,922 + thrB MCAODC_01620 0.52 -0.3 313,923 - thrB MCAODC_01620 0.52 +0.8 314,018 + thrB MCAODC_01620 0.62 -0.7 314,085 + thrB MCAODC_01620 0.69 -1.3 314,176 + thrB MCAODC_01620 0.79 -0.5 314,268 + thrB MCAODC_01620 0.89 -0.4 314,268 + thrB MCAODC_01620 0.89 -1.4 314,268 + thrB MCAODC_01620 0.89 +0.8 314,269 - thrB MCAODC_01620 0.89 -1.4 314,269 - thrB MCAODC_01620 0.89 +3.3 314,269 - thrB MCAODC_01620 0.89 -1.3 314,269 - thrB MCAODC_01620 0.89 -0.1 314,269 - thrB MCAODC_01620 0.89 +0.4 314,269 - thrB MCAODC_01620 0.89 -0.0 314,269 - thrB MCAODC_01620 0.89 -0.5 314,269 - thrB MCAODC_01620 0.89 -0.7 314,269 - thrB MCAODC_01620 0.89 +0.6 314,269 - thrB MCAODC_01620 0.89 +0.9 314,336 + -1.9 314,337 - -1.5 314,384 - -2.7 314,384 - -0.5 314,401 - -0.3 314,401 - +0.0 314,401 - +0.4 314,404 + -0.9 314,404 + -1.7 314,405 - +0.7 314,405 - +2.6 314,405 - +0.1 314,484 - +0.3 314,486 + -0.8 314,486 + -0.3 314,487 - -0.6 314,487 - -1.5 314,495 + -0.8 314,496 - -0.5 314,592 + -0.4 314,603 + -0.7 314,604 - -1.8 314,604 - -1.7 314,604 - -1.4 314,683 - thrA MCAODC_01625 0.13 -0.0 314,683 - thrA MCAODC_01625 0.13 +0.4 314,786 - thrA MCAODC_01625 0.17 -0.3 314,788 + thrA MCAODC_01625 0.17 +1.0 314,788 + thrA MCAODC_01625 0.17 -0.8 314,789 - thrA MCAODC_01625 0.17 -0.9 314,793 + thrA MCAODC_01625 0.17 +0.6 314,794 - thrA MCAODC_01625 0.17 -2.1 314,794 - thrA MCAODC_01625 0.17 +1.7 314,794 - thrA MCAODC_01625 0.17 -0.3 314,800 + thrA MCAODC_01625 0.17 -0.0 314,801 - thrA MCAODC_01625 0.17 -1.6 314,801 - thrA MCAODC_01625 0.17 -0.1 314,801 - thrA MCAODC_01625 0.17 +2.0 314,844 - thrA MCAODC_01625 0.19 -3.0 314,910 + thrA MCAODC_01625 0.22 +0.4 314,911 - thrA MCAODC_01625 0.22 +2.0 314,911 - thrA MCAODC_01625 0.22 +0.5 314,911 - thrA MCAODC_01625 0.22 -0.4 314,912 + thrA MCAODC_01625 0.22 -0.9 314,912 + thrA MCAODC_01625 0.22 +0.4 314,912 + thrA MCAODC_01625 0.22 -2.7 314,912 + thrA MCAODC_01625 0.22 -0.6 314,912 + thrA MCAODC_01625 0.22 -0.3 314,912 + thrA MCAODC_01625 0.22 +0.4 314,913 - thrA MCAODC_01625 0.22 -1.8 314,913 - thrA MCAODC_01625 0.22 +1.1 315,024 - thrA MCAODC_01625 0.26 -1.7 315,024 - thrA MCAODC_01625 0.26 -2.3 315,071 + thrA MCAODC_01625 0.28 -0.1 315,072 - thrA MCAODC_01625 0.28 +0.4 315,072 - thrA MCAODC_01625 0.28 -1.6 315,075 - thrA MCAODC_01625 0.28 -2.0 315,146 + thrA MCAODC_01625 0.31 +1.0 315,146 + thrA MCAODC_01625 0.31 +0.1 315,206 + thrA MCAODC_01625 0.34 +0.5 315,240 + thrA MCAODC_01625 0.35 +1.7 315,290 - thrA MCAODC_01625 0.37 -0.8 315,324 + thrA MCAODC_01625 0.39 -0.8 315,343 + thrA MCAODC_01625 0.39 +0.9 315,343 + thrA MCAODC_01625 0.39 -1.3 315,343 + thrA MCAODC_01625 0.39 -0.8 315,343 + thrA MCAODC_01625 0.39 +0.3 315,343 + thrA MCAODC_01625 0.39 -0.6 315,343 + thrA MCAODC_01625 0.39 -2.5 315,343 + thrA MCAODC_01625 0.39 +0.4 315,344 - thrA MCAODC_01625 0.39 +0.2 315,344 - thrA MCAODC_01625 0.39 -2.3 315,344 - thrA MCAODC_01625 0.39 -1.5 315,351 + thrA MCAODC_01625 0.40 +2.0 315,352 - thrA MCAODC_01625 0.40 -1.0
Or see this region's nucleotide sequence