Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafP and yafO are separated by 84 nucleotides yafO and yafN are separated by 2 nucleotides yafN and dinB are separated by 51 nucleotides dinB and lafU are separated by 70 nucleotides lafU and lfhA are separated by 94 nucleotides
MCAODC_00235: yafP - GNAT family N-acetyltransferase, at 23,547 to 23,924
yafP
MCAODC_00240: yafO - type II toxin-antitoxin system mRNA interferase toxin YafO, at 24,009 to 24,407
yafO
MCAODC_00245: yafN - type I toxin-antitoxin system antitoxin YafN, at 24,410 to 24,703
yafN
MCAODC_00250: dinB - DNA polymerase IV, at 24,755 to 25,810
dinB
MCAODC_00255: lafU - putative lateral flagellar export/assembly protein LafU, at 25,881 to 26,516
lafU
MCAODC_00260: lfhA - Putative truncated flagellar export/assembly protein LfhA, at 26,611 to 28,350
lfhA
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.769 kb on - strand, within yafP at 23.769 kb on - strand, within yafP at 23.770 kb on + strand, within yafP at 23.950 kb on - strand at 23.975 kb on - strand at 24.051 kb on - strand, within yafO at 24.145 kb on + strand, within yafO at 24.146 kb on - strand, within yafO at 24.146 kb on - strand, within yafO at 24.228 kb on - strand, within yafO at 24.297 kb on + strand, within yafO at 24.297 kb on + strand, within yafO at 24.298 kb on - strand, within yafO at 24.298 kb on - strand, within yafO at 24.326 kb on - strand, within yafO at 24.419 kb on - strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.719 kb on + strand at 24.743 kb on + strand at 24.744 kb on - strand at 24.746 kb on + strand at 24.747 kb on - strand at 24.749 kb on + strand at 24.749 kb on + strand at 24.751 kb on + strand at 24.751 kb on + strand at 24.875 kb on + strand, within dinB at 24.980 kb on + strand, within dinB at 24.980 kb on + strand, within dinB at 25.054 kb on + strand, within dinB at 25.136 kb on - strand, within dinB at 25.342 kb on + strand, within dinB at 25.494 kb on + strand, within dinB at 25.495 kb on - strand, within dinB at 25.653 kb on - strand, within dinB at 25.675 kb on - strand, within dinB at 25.726 kb on + strand at 25.839 kb on - strand at 25.867 kb on + strand at 25.868 kb on - strand at 25.966 kb on + strand, within lafU at 25.966 kb on + strand, within lafU at 25.967 kb on - strand, within lafU at 25.967 kb on - strand, within lafU at 25.972 kb on + strand, within lafU at 25.972 kb on + strand, within lafU at 25.973 kb on - strand, within lafU at 26.006 kb on + strand, within lafU at 26.079 kb on + strand, within lafU at 26.094 kb on - strand, within lafU at 26.170 kb on + strand, within lafU at 26.170 kb on + strand, within lafU at 26.170 kb on + strand, within lafU at 26.171 kb on - strand, within lafU at 26.186 kb on - strand, within lafU at 26.211 kb on - strand, within lafU at 26.216 kb on + strand, within lafU at 26.283 kb on - strand, within lafU at 26.331 kb on - strand, within lafU at 26.384 kb on + strand, within lafU at 26.556 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 23,769 - yafP MCAODC_00235 0.59 -0.8 23,769 - yafP MCAODC_00235 0.59 -0.5 23,770 + yafP MCAODC_00235 0.59 +2.7 23,950 - +0.5 23,975 - +1.0 24,051 - yafO MCAODC_00240 0.11 +0.4 24,145 + yafO MCAODC_00240 0.34 +0.0 24,146 - yafO MCAODC_00240 0.34 -0.1 24,146 - yafO MCAODC_00240 0.34 +2.0 24,228 - yafO MCAODC_00240 0.55 +1.4 24,297 + yafO MCAODC_00240 0.72 +0.2 24,297 + yafO MCAODC_00240 0.72 -0.5 24,298 - yafO MCAODC_00240 0.72 +1.0 24,298 - yafO MCAODC_00240 0.72 -0.1 24,326 - yafO MCAODC_00240 0.79 -0.8 24,419 - +2.0 24,703 + -1.5 24,703 + +0.6 24,719 + -0.8 24,743 + +1.0 24,744 - +1.0 24,746 + +1.0 24,747 - -1.0 24,749 + -1.0 24,749 + -1.7 24,751 + +0.7 24,751 + +1.2 24,875 + dinB MCAODC_00250 0.11 -0.0 24,980 + dinB MCAODC_00250 0.21 +0.1 24,980 + dinB MCAODC_00250 0.21 +1.1 25,054 + dinB MCAODC_00250 0.28 -2.0 25,136 - dinB MCAODC_00250 0.36 -2.1 25,342 + dinB MCAODC_00250 0.56 +0.9 25,494 + dinB MCAODC_00250 0.70 +1.1 25,495 - dinB MCAODC_00250 0.70 +0.1 25,653 - dinB MCAODC_00250 0.85 -0.0 25,675 - dinB MCAODC_00250 0.87 -0.0 25,726 + -0.8 25,839 - +1.1 25,867 + -1.1 25,868 - +0.8 25,966 + lafU MCAODC_00255 0.13 +0.4 25,966 + lafU MCAODC_00255 0.13 +0.0 25,967 - lafU MCAODC_00255 0.14 +1.4 25,967 - lafU MCAODC_00255 0.14 +0.5 25,972 + lafU MCAODC_00255 0.14 -0.8 25,972 + lafU MCAODC_00255 0.14 +0.1 25,973 - lafU MCAODC_00255 0.14 +1.0 26,006 + lafU MCAODC_00255 0.20 +0.6 26,079 + lafU MCAODC_00255 0.31 -1.8 26,094 - lafU MCAODC_00255 0.33 -0.1 26,170 + lafU MCAODC_00255 0.45 +0.8 26,170 + lafU MCAODC_00255 0.45 +1.1 26,170 + lafU MCAODC_00255 0.45 -0.9 26,171 - lafU MCAODC_00255 0.46 -0.4 26,186 - lafU MCAODC_00255 0.48 +1.2 26,211 - lafU MCAODC_00255 0.52 -0.1 26,216 + lafU MCAODC_00255 0.53 +0.2 26,283 - lafU MCAODC_00255 0.63 +1.0 26,331 - lafU MCAODC_00255 0.71 -0.5 26,384 + lafU MCAODC_00255 0.79 -0.5 26,556 + +0.1
Or see this region's nucleotide sequence