Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepD and prfH are separated by 56 nucleotides prfH and ykfJ overlap by 32 nucleotides ykfJ and yafP are separated by 306 nucleotides
MCAODC_00220: pepD - cytosol nonspecific dipeptidase, at 20,991 to 22,448
pepD
MCAODC_00225: prfH - peptide chain release factor H, at 22,505 to 23,005
prfH
MCAODC_00230: ykfJ - Putative uncharacterized protein YkfJ, at 22,974 to 23,240
ykfJ
MCAODC_00235: yafP - GNAT family N-acetyltransferase, at 23,547 to 23,924
yafP
Position (kb) 22
23
24 Strain fitness (log2 ratio) -2
-1
0
1
2 at 21.519 kb on + strand, within pepD at 21.519 kb on + strand, within pepD at 21.519 kb on + strand, within pepD at 21.566 kb on - strand, within pepD at 21.613 kb on + strand, within pepD at 21.613 kb on + strand, within pepD at 21.614 kb on - strand, within pepD at 21.614 kb on - strand, within pepD at 21.614 kb on - strand, within pepD at 21.834 kb on - strand, within pepD at 21.834 kb on - strand, within pepD at 21.834 kb on - strand, within pepD at 21.834 kb on - strand, within pepD at 21.837 kb on + strand, within pepD at 21.838 kb on - strand, within pepD at 21.838 kb on - strand, within pepD at 21.838 kb on - strand, within pepD at 21.838 kb on - strand, within pepD at 21.914 kb on + strand, within pepD at 21.914 kb on + strand, within pepD at 21.951 kb on - strand, within pepD at 22.063 kb on + strand, within pepD at 22.110 kb on + strand, within pepD at 22.111 kb on - strand, within pepD at 22.111 kb on - strand, within pepD at 22.111 kb on - strand, within pepD at 22.176 kb on + strand, within pepD at 22.222 kb on + strand, within pepD at 22.334 kb on + strand at 22.393 kb on + strand at 22.393 kb on + strand at 22.393 kb on + strand at 22.394 kb on - strand at 22.447 kb on - strand at 22.451 kb on - strand at 22.517 kb on - strand at 22.603 kb on + strand, within prfH at 22.604 kb on - strand, within prfH at 22.604 kb on - strand, within prfH at 22.664 kb on - strand, within prfH at 22.728 kb on - strand, within prfH at 22.825 kb on + strand, within prfH at 22.825 kb on + strand, within prfH at 22.981 kb on - strand at 23.085 kb on + strand, within ykfJ at 23.103 kb on + strand, within ykfJ at 23.140 kb on - strand, within ykfJ at 23.189 kb on + strand, within ykfJ at 23.190 kb on - strand, within ykfJ at 23.190 kb on - strand, within ykfJ at 23.194 kb on - strand, within ykfJ at 23.216 kb on + strand at 23.217 kb on - strand at 23.218 kb on + strand at 23.219 kb on - strand at 23.231 kb on - strand at 23.242 kb on + strand at 23.242 kb on + strand at 23.242 kb on + strand at 23.243 kb on - strand at 23.254 kb on - strand at 23.254 kb on - strand at 23.306 kb on - strand at 23.310 kb on - strand at 23.369 kb on + strand at 23.563 kb on + strand at 23.619 kb on - strand, within yafP at 23.619 kb on - strand, within yafP at 23.679 kb on - strand, within yafP at 23.696 kb on + strand, within yafP at 23.769 kb on - strand, within yafP at 23.769 kb on - strand, within yafP at 23.770 kb on + strand, within yafP at 23.950 kb on - strand at 23.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 21,519 + pepD MCAODC_00220 0.36 -0.8 21,519 + pepD MCAODC_00220 0.36 +0.7 21,519 + pepD MCAODC_00220 0.36 -0.2 21,566 - pepD MCAODC_00220 0.39 +0.5 21,613 + pepD MCAODC_00220 0.43 +0.2 21,613 + pepD MCAODC_00220 0.43 +0.7 21,614 - pepD MCAODC_00220 0.43 -0.2 21,614 - pepD MCAODC_00220 0.43 -2.3 21,614 - pepD MCAODC_00220 0.43 -1.0 21,834 - pepD MCAODC_00220 0.58 +0.6 21,834 - pepD MCAODC_00220 0.58 +0.4 21,834 - pepD MCAODC_00220 0.58 +0.8 21,834 - pepD MCAODC_00220 0.58 -0.7 21,837 + pepD MCAODC_00220 0.58 +2.0 21,838 - pepD MCAODC_00220 0.58 +0.5 21,838 - pepD MCAODC_00220 0.58 +0.7 21,838 - pepD MCAODC_00220 0.58 +0.3 21,838 - pepD MCAODC_00220 0.58 -0.5 21,914 + pepD MCAODC_00220 0.63 +0.3 21,914 + pepD MCAODC_00220 0.63 -1.4 21,951 - pepD MCAODC_00220 0.66 -1.8 22,063 + pepD MCAODC_00220 0.74 +0.4 22,110 + pepD MCAODC_00220 0.77 -2.3 22,111 - pepD MCAODC_00220 0.77 +0.4 22,111 - pepD MCAODC_00220 0.77 -0.8 22,111 - pepD MCAODC_00220 0.77 +0.2 22,176 + pepD MCAODC_00220 0.81 -0.5 22,222 + pepD MCAODC_00220 0.84 -0.6 22,334 + -0.6 22,393 + -0.8 22,393 + -0.2 22,393 + -0.6 22,394 - +0.6 22,447 - -0.5 22,451 - +1.4 22,517 - -1.6 22,603 + prfH MCAODC_00225 0.20 +0.4 22,604 - prfH MCAODC_00225 0.20 -1.6 22,604 - prfH MCAODC_00225 0.20 +0.5 22,664 - prfH MCAODC_00225 0.32 -0.8 22,728 - prfH MCAODC_00225 0.45 +0.6 22,825 + prfH MCAODC_00225 0.64 -0.4 22,825 + prfH MCAODC_00225 0.64 -0.6 22,981 - +1.4 23,085 + ykfJ MCAODC_00230 0.42 -0.4 23,103 + ykfJ MCAODC_00230 0.48 -0.6 23,140 - ykfJ MCAODC_00230 0.62 -0.0 23,189 + ykfJ MCAODC_00230 0.81 -0.8 23,190 - ykfJ MCAODC_00230 0.81 +0.9 23,190 - ykfJ MCAODC_00230 0.81 -0.7 23,194 - ykfJ MCAODC_00230 0.82 -0.4 23,216 + +0.7 23,217 - +1.3 23,218 + +0.6 23,219 - -1.7 23,231 - -0.6 23,242 + -1.0 23,242 + +2.3 23,242 + +1.0 23,243 - +0.6 23,254 - -1.7 23,254 - -0.1 23,306 - +0.1 23,310 - +0.2 23,369 + +0.6 23,563 + +1.0 23,619 - yafP MCAODC_00235 0.19 -0.2 23,619 - yafP MCAODC_00235 0.19 +0.1 23,679 - yafP MCAODC_00235 0.35 -0.1 23,696 + yafP MCAODC_00235 0.39 -1.4 23,769 - yafP MCAODC_00235 0.59 -0.8 23,769 - yafP MCAODC_00235 0.59 -0.5 23,770 + yafP MCAODC_00235 0.59 +2.7 23,950 - +0.5 23,975 - +1.0
Or see this region's nucleotide sequence