Experiment: Ying_Nucleobase14 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5634 and GFF5635 are separated by 21 nucleotides GFF5635 and GFF5636 are separated by 5 nucleotides GFF5636 and GFF5637 overlap by 4 nucleotides
GFF5634 - Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1), at 2,655,086 to 2,656,252
GFF5634
GFF5635 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,656,274 to 2,658,118
GFF5635
GFF5636 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 2,658,124 to 2,658,897
GFF5636
GFF5637 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,658,894 to 2,659,706
GFF5637
Position (kb)
2656
2657
2658
2659 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2655.364 kb on - strand, within GFF5634 at 2655.807 kb on + strand, within GFF5634 at 2655.807 kb on + strand, within GFF5634 at 2655.825 kb on + strand, within GFF5634 at 2655.831 kb on + strand, within GFF5634 at 2655.831 kb on + strand, within GFF5634 at 2655.831 kb on + strand, within GFF5634 at 2655.850 kb on - strand, within GFF5634 at 2656.491 kb on + strand, within GFF5635 at 2657.358 kb on + strand, within GFF5635 at 2657.520 kb on + strand, within GFF5635 at 2657.773 kb on - strand, within GFF5635 at 2657.773 kb on - strand, within GFF5635 at 2658.953 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 1 remove 2,655,364 - GFF5634 0.24 -1.4 2,655,807 + GFF5634 0.62 +0.2 2,655,807 + GFF5634 0.62 -0.9 2,655,825 + GFF5634 0.63 +0.2 2,655,831 + GFF5634 0.64 +0.1 2,655,831 + GFF5634 0.64 +0.1 2,655,831 + GFF5634 0.64 -0.2 2,655,850 - GFF5634 0.65 -0.1 2,656,491 + GFF5635 0.12 -1.2 2,657,358 + GFF5635 0.59 +0.6 2,657,520 + GFF5635 0.68 +2.6 2,657,773 - GFF5635 0.81 -2.2 2,657,773 - GFF5635 0.81 +0.3 2,658,953 - -0.2
Or see this region's nucleotide sequence