Experiment: Ying_Nucleobase14 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2998 and GFF2999 are separated by 24 nucleotides GFF2999 and GFF3000 are separated by 1 nucleotides GFF3000 and GFF3001 overlap by 4 nucleotides GFF3001 and GFF3002 overlap by 14 nucleotides GFF3002 and GFF3003 overlap by 4 nucleotides
GFF2998 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41), at 76,855 to 78,120
GFF2998
GFF2999 - Acyl carrier protein (ACP1), at 78,145 to 78,429
GFF2999
GFF3000 - FIG017861: hypothetical protein, at 78,431 to 79,093
GFF3000
GFF3001 - FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase, at 79,090 to 80,436
GFF3001
GFF3002 - (3R)-hydroxymyristoyl-[ACP] dehydratase (EC 4.2.1.-), at 80,423 to 80,731
GFF3002
GFF3003 - Lysophospholipid acyltransferase, at 80,728 to 81,633
GFF3003
Position (kb)
79
80
81 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 78.331 kb on + strand, within GFF2999 at 78.332 kb on - strand, within GFF2999 at 78.689 kb on + strand, within GFF3000 at 78.689 kb on + strand, within GFF3000 at 78.689 kb on + strand, within GFF3000 at 78.689 kb on + strand, within GFF3000 at 78.689 kb on + strand, within GFF3000 at 78.689 kb on + strand, within GFF3000 at 78.689 kb on + strand, within GFF3000 at 78.690 kb on - strand, within GFF3000 at 78.690 kb on - strand, within GFF3000 at 78.690 kb on - strand, within GFF3000 at 78.690 kb on - strand, within GFF3000 at 78.690 kb on - strand, within GFF3000 at 78.690 kb on - strand, within GFF3000 at 78.690 kb on - strand, within GFF3000 at 78.690 kb on - strand, within GFF3000 at 78.692 kb on + strand, within GFF3000 at 78.692 kb on + strand, within GFF3000 at 78.692 kb on + strand, within GFF3000 at 78.692 kb on + strand, within GFF3000 at 78.692 kb on + strand, within GFF3000 at 78.693 kb on - strand, within GFF3000 at 78.693 kb on - strand, within GFF3000 at 78.693 kb on - strand, within GFF3000 at 79.046 kb on + strand at 79.046 kb on + strand at 79.047 kb on - strand at 79.047 kb on - strand at 79.047 kb on - strand at 79.253 kb on - strand, within GFF3001 at 79.354 kb on + strand, within GFF3001 at 79.354 kb on + strand, within GFF3001 at 79.642 kb on + strand, within GFF3001 at 79.642 kb on + strand, within GFF3001 at 79.642 kb on + strand, within GFF3001 at 79.643 kb on - strand, within GFF3001 at 79.711 kb on + strand, within GFF3001 at 79.711 kb on + strand, within GFF3001 at 79.712 kb on - strand, within GFF3001 at 80.017 kb on + strand, within GFF3001 at 80.017 kb on + strand, within GFF3001 at 80.017 kb on + strand, within GFF3001 at 80.017 kb on + strand, within GFF3001 at 80.017 kb on + strand, within GFF3001 at 80.018 kb on - strand, within GFF3001 at 80.018 kb on - strand, within GFF3001 at 80.018 kb on - strand, within GFF3001 at 80.153 kb on - strand, within GFF3001 at 80.153 kb on - strand, within GFF3001 at 80.153 kb on - strand, within GFF3001 at 80.863 kb on + strand, within GFF3003 at 80.863 kb on + strand, within GFF3003 at 80.863 kb on + strand, within GFF3003 at 80.864 kb on - strand, within GFF3003 at 80.909 kb on - strand, within GFF3003 at 80.909 kb on - strand, within GFF3003 at 80.909 kb on - strand, within GFF3003 at 80.909 kb on - strand, within GFF3003 at 80.999 kb on - strand, within GFF3003 at 80.999 kb on - strand, within GFF3003 at 81.175 kb on + strand, within GFF3003 at 81.175 kb on + strand, within GFF3003 at 81.176 kb on - strand, within GFF3003
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 1 remove 78,331 + GFF2999 0.65 +0.2 78,332 - GFF2999 0.66 -0.1 78,689 + GFF3000 0.39 +3.2 78,689 + GFF3000 0.39 -0.2 78,689 + GFF3000 0.39 +0.7 78,689 + GFF3000 0.39 -0.0 78,689 + GFF3000 0.39 -0.1 78,689 + GFF3000 0.39 -1.8 78,689 + GFF3000 0.39 +0.0 78,690 - GFF3000 0.39 -2.8 78,690 - GFF3000 0.39 +0.7 78,690 - GFF3000 0.39 +0.6 78,690 - GFF3000 0.39 -0.2 78,690 - GFF3000 0.39 -0.3 78,690 - GFF3000 0.39 +0.1 78,690 - GFF3000 0.39 -0.8 78,690 - GFF3000 0.39 -0.3 78,692 + GFF3000 0.39 +1.1 78,692 + GFF3000 0.39 +0.2 78,692 + GFF3000 0.39 -0.3 78,692 + GFF3000 0.39 +0.0 78,692 + GFF3000 0.39 -0.2 78,693 - GFF3000 0.40 -0.1 78,693 - GFF3000 0.40 -1.2 78,693 - GFF3000 0.40 -0.2 79,046 + +0.4 79,046 + -0.9 79,047 - -0.2 79,047 - -0.6 79,047 - -0.5 79,253 - GFF3001 0.12 -2.5 79,354 + GFF3001 0.20 +0.3 79,354 + GFF3001 0.20 -0.5 79,642 + GFF3001 0.41 +0.2 79,642 + GFF3001 0.41 +0.0 79,642 + GFF3001 0.41 +0.5 79,643 - GFF3001 0.41 +0.8 79,711 + GFF3001 0.46 +0.1 79,711 + GFF3001 0.46 +0.1 79,712 - GFF3001 0.46 -0.8 80,017 + GFF3001 0.69 -0.1 80,017 + GFF3001 0.69 -0.1 80,017 + GFF3001 0.69 +0.0 80,017 + GFF3001 0.69 -0.9 80,017 + GFF3001 0.69 -0.1 80,018 - GFF3001 0.69 -0.5 80,018 - GFF3001 0.69 +0.2 80,018 - GFF3001 0.69 -1.1 80,153 - GFF3001 0.79 -1.5 80,153 - GFF3001 0.79 -1.9 80,153 - GFF3001 0.79 -0.9 80,863 + GFF3003 0.15 +1.2 80,863 + GFF3003 0.15 -0.2 80,863 + GFF3003 0.15 +0.1 80,864 - GFF3003 0.15 -0.1 80,909 - GFF3003 0.20 -0.3 80,909 - GFF3003 0.20 +0.1 80,909 - GFF3003 0.20 +1.4 80,909 - GFF3003 0.20 +0.1 80,999 - GFF3003 0.30 -0.3 80,999 - GFF3003 0.30 +0.5 81,175 + GFF3003 0.49 -1.1 81,175 + GFF3003 0.49 +0.4 81,176 - GFF3003 0.49 -1.0
Or see this region's nucleotide sequence