Experiment: Ying_Nucleobase14 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF230 and GFF231 are separated by 4 nucleotides GFF231 and GFF232 overlap by 4 nucleotides GFF232 and GFF233 overlap by 1 nucleotides GFF233 and GFF234 are separated by 90 nucleotides
GFF230 - Thioredoxin reductase (EC 1.8.1.9), at 251,736 to 252,635
GFF230
GFF231 - probable DNA repair exonuclease, at 252,640 to 253,428
GFF231
GFF232 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 253,425 to 254,396
GFF232
GFF233 - hypothetical protein, at 254,396 to 254,944
GFF233
GFF234 - Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit, at 255,035 to 256,204
GFF234
Position (kb) 253
254
255 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 252.854 kb on + strand, within GFF231 at 252.855 kb on - strand, within GFF231 at 252.855 kb on - strand, within GFF231 at 252.855 kb on - strand, within GFF231 at 252.855 kb on - strand, within GFF231 at 252.855 kb on - strand, within GFF231 at 252.855 kb on - strand, within GFF231 at 252.855 kb on - strand, within GFF231 at 252.855 kb on - strand, within GFF231 at 253.172 kb on + strand, within GFF231 at 253.209 kb on + strand, within GFF231 at 253.948 kb on + strand, within GFF232 at 254.083 kb on + strand, within GFF232 at 254.083 kb on + strand, within GFF232 at 254.084 kb on - strand, within GFF232 at 254.448 kb on + strand at 254.796 kb on + strand, within GFF233 at 254.985 kb on + strand at 254.985 kb on + strand at 254.986 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 1 remove 252,854 + GFF231 0.27 -2.4 252,855 - GFF231 0.27 +0.1 252,855 - GFF231 0.27 -0.6 252,855 - GFF231 0.27 -0.8 252,855 - GFF231 0.27 +0.0 252,855 - GFF231 0.27 -1.2 252,855 - GFF231 0.27 -0.3 252,855 - GFF231 0.27 -0.7 252,855 - GFF231 0.27 +0.0 253,172 + GFF231 0.67 -0.4 253,209 + GFF231 0.72 -0.1 253,948 + GFF232 0.54 -0.4 254,083 + GFF232 0.68 -2.8 254,083 + GFF232 0.68 -0.3 254,084 - GFF232 0.68 -0.7 254,448 + -0.2 254,796 + GFF233 0.73 -0.4 254,985 + +0.2 254,985 + -0.1 254,986 - -0.0
Or see this region's nucleotide sequence