Experiment: Ying_Nucleobase14 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1324 and GFF1325 overlap by 4 nucleotides GFF1325 and GFF1326 are separated by 81 nucleotides GFF1326 and GFF1327 are separated by 35 nucleotides
GFF1324 - Uncharacterized ABC transporter, permease component YrbE, at 172,478 to 173,260
GFF1324
GFF1325 - Methionine ABC transporter ATP-binding protein, at 173,257 to 174,084
GFF1325
GFF1326 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 174,166 to 175,632
GFF1326
GFF1327 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 175,668 to 180,446
GFF1327
Position (kb) 174
175
176 Strain fitness (log2 ratio) -2
-1
0
1 at 173.579 kb on + strand, within GFF1325 at 173.579 kb on + strand, within GFF1325 at 173.580 kb on - strand, within GFF1325 at 173.840 kb on + strand, within GFF1325 at 173.840 kb on + strand, within GFF1325 at 173.840 kb on + strand, within GFF1325 at 173.841 kb on - strand, within GFF1325 at 174.126 kb on + strand at 174.126 kb on + strand at 174.126 kb on + strand at 174.164 kb on + strand at 174.165 kb on - strand at 174.308 kb on + strand at 174.308 kb on + strand at 174.308 kb on + strand at 174.308 kb on + strand at 174.308 kb on + strand at 174.309 kb on - strand at 174.434 kb on + strand, within GFF1326 at 174.434 kb on + strand, within GFF1326 at 174.434 kb on + strand, within GFF1326 at 174.434 kb on + strand, within GFF1326 at 174.434 kb on + strand, within GFF1326 at 174.434 kb on + strand, within GFF1326 at 174.434 kb on + strand, within GFF1326 at 174.434 kb on + strand, within GFF1326 at 174.434 kb on + strand, within GFF1326 at 174.434 kb on + strand, within GFF1326 at 174.435 kb on - strand, within GFF1326 at 174.435 kb on - strand, within GFF1326 at 174.435 kb on - strand, within GFF1326 at 174.435 kb on - strand, within GFF1326 at 174.435 kb on - strand, within GFF1326 at 174.435 kb on - strand, within GFF1326 at 174.608 kb on + strand, within GFF1326 at 175.418 kb on + strand, within GFF1326 at 175.553 kb on + strand at 175.553 kb on + strand at 175.553 kb on + strand at 175.553 kb on + strand at 175.554 kb on - strand at 175.554 kb on - strand at 175.554 kb on - strand at 175.583 kb on + strand at 175.583 kb on + strand at 175.583 kb on + strand at 175.583 kb on + strand at 175.583 kb on + strand at 175.584 kb on - strand at 175.584 kb on - strand at 175.584 kb on - strand at 175.604 kb on + strand at 175.604 kb on + strand at 175.605 kb on - strand at 175.605 kb on - strand at 175.669 kb on + strand at 175.670 kb on - strand at 175.670 kb on - strand at 175.670 kb on - strand at 175.768 kb on + strand at 175.768 kb on + strand at 175.768 kb on + strand at 175.768 kb on + strand at 175.768 kb on + strand at 175.769 kb on - strand at 175.769 kb on - strand at 175.769 kb on - strand at 175.769 kb on - strand at 175.769 kb on - strand at 175.769 kb on - strand at 175.769 kb on - strand at 176.017 kb on + strand at 176.018 kb on - strand at 176.018 kb on - strand at 176.018 kb on - strand at 176.023 kb on + strand at 176.023 kb on + strand at 176.023 kb on + strand at 176.023 kb on + strand at 176.023 kb on + strand at 176.023 kb on + strand at 176.023 kb on + strand at 176.024 kb on - strand at 176.024 kb on - strand at 176.024 kb on - strand at 176.024 kb on - strand at 176.024 kb on - strand at 176.024 kb on - strand at 176.024 kb on - strand at 176.024 kb on - strand at 176.024 kb on - strand at 176.101 kb on + strand at 176.101 kb on + strand at 176.101 kb on + strand at 176.101 kb on + strand at 176.101 kb on + strand at 176.101 kb on + strand at 176.101 kb on + strand at 176.102 kb on - strand at 176.102 kb on - strand at 176.102 kb on - strand at 176.102 kb on - strand at 176.215 kb on + strand, within GFF1327 at 176.216 kb on - strand, within GFF1327 at 176.216 kb on - strand, within GFF1327 at 176.318 kb on - strand, within GFF1327 at 176.318 kb on - strand, within GFF1327 at 176.318 kb on - strand, within GFF1327 at 176.341 kb on + strand, within GFF1327 at 176.341 kb on + strand, within GFF1327 at 176.342 kb on - strand, within GFF1327 at 176.342 kb on - strand, within GFF1327 at 176.342 kb on - strand, within GFF1327
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 1 remove 173,579 + GFF1325 0.39 -1.8 173,579 + GFF1325 0.39 -0.9 173,580 - GFF1325 0.39 -1.3 173,840 + GFF1325 0.70 -0.8 173,840 + GFF1325 0.70 -0.8 173,840 + GFF1325 0.70 -2.5 173,841 - GFF1325 0.71 -0.4 174,126 + -0.9 174,126 + -0.9 174,126 + +0.4 174,164 + -0.1 174,165 - -1.2 174,308 + -1.2 174,308 + -0.2 174,308 + -1.9 174,308 + -0.7 174,308 + -0.7 174,309 - -0.6 174,434 + GFF1326 0.18 +0.3 174,434 + GFF1326 0.18 -0.4 174,434 + GFF1326 0.18 -0.0 174,434 + GFF1326 0.18 -0.8 174,434 + GFF1326 0.18 +0.0 174,434 + GFF1326 0.18 +0.2 174,434 + GFF1326 0.18 -0.7 174,434 + GFF1326 0.18 +0.8 174,434 + GFF1326 0.18 -0.4 174,434 + GFF1326 0.18 -1.3 174,435 - GFF1326 0.18 -1.1 174,435 - GFF1326 0.18 -0.3 174,435 - GFF1326 0.18 -0.5 174,435 - GFF1326 0.18 -0.8 174,435 - GFF1326 0.18 -0.3 174,435 - GFF1326 0.18 -0.2 174,608 + GFF1326 0.30 -0.7 175,418 + GFF1326 0.85 -0.2 175,553 + -0.5 175,553 + -0.2 175,553 + +0.2 175,553 + -1.2 175,554 - +0.5 175,554 - -1.3 175,554 - -1.1 175,583 + -2.1 175,583 + +0.4 175,583 + -0.4 175,583 + -0.4 175,583 + -0.5 175,584 - -0.3 175,584 - -0.2 175,584 - +0.3 175,604 + -0.6 175,604 + -0.5 175,605 - -1.4 175,605 - -0.5 175,669 + +0.4 175,670 - +0.2 175,670 - -0.3 175,670 - -0.2 175,768 + -0.0 175,768 + +0.1 175,768 + +0.2 175,768 + -0.5 175,768 + +0.1 175,769 - -1.0 175,769 - -0.5 175,769 - -0.9 175,769 - -0.1 175,769 - -0.2 175,769 - -1.2 175,769 - -0.0 176,017 + -0.0 176,018 - -1.1 176,018 - -0.3 176,018 - -1.4 176,023 + -0.2 176,023 + -0.7 176,023 + -1.3 176,023 + -1.6 176,023 + -0.7 176,023 + -0.6 176,023 + -0.9 176,024 - +0.2 176,024 - -2.4 176,024 - -1.2 176,024 - -0.7 176,024 - +0.1 176,024 - -0.7 176,024 - -0.5 176,024 - -1.0 176,024 - -0.5 176,101 + -0.3 176,101 + -0.1 176,101 + +0.1 176,101 + -0.7 176,101 + +0.3 176,101 + -0.0 176,101 + -0.4 176,102 - -0.7 176,102 - -0.7 176,102 - -1.6 176,102 - -1.0 176,215 + GFF1327 0.11 -0.8 176,216 - GFF1327 0.11 -0.4 176,216 - GFF1327 0.11 +0.2 176,318 - GFF1327 0.14 +0.1 176,318 - GFF1327 0.14 -0.7 176,318 - GFF1327 0.14 -0.9 176,341 + GFF1327 0.14 -0.7 176,341 + GFF1327 0.14 -0.7 176,342 - GFF1327 0.14 +0.0 176,342 - GFF1327 0.14 -0.0 176,342 - GFF1327 0.14 +0.6
Or see this region's nucleotide sequence