Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF5226

Experiment: Ying_OrganicAcid7 rep C; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5224 and GFF5225 are separated by 9 nucleotidesGFF5225 and GFF5226 are separated by 77 nucleotidesGFF5226 and GFF5227 overlap by 4 nucleotidesGFF5227 and GFF5228 are separated by 37 nucleotides GFF5224 - Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1), at 2,240,488 to 2,242,104 GFF5224 GFF5225 - 2-hydroxychromene-2-carboxylate isomerase/DsbA-like thioredoxin domain, at 2,242,114 to 2,242,779 GFF5225 GFF5226 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 2,242,857 to 2,243,678 GFF5226 GFF5227 - Permease of the drug/metabolite transporter (DMT) superfamily, at 2,243,675 to 2,244,556 GFF5227 GFF5228 - Transcriptional regulator, TetR family, at 2,244,594 to 2,245,274 GFF5228 Position (kb) 2242 2243 2244Strain fitness (log2 ratio) -3 -2 -1 0 1at 2242.012 kb on + strandat 2242.012 kb on + strandat 2242.045 kb on + strandat 2242.046 kb on - strandat 2242.046 kb on - strandat 2242.046 kb on - strandat 2242.046 kb on - strandat 2242.100 kb on - strandat 2242.100 kb on - strandat 2242.100 kb on - strandat 2242.100 kb on - strandat 2242.100 kb on - strandat 2242.100 kb on - strandat 2242.115 kb on + strandat 2242.115 kb on + strandat 2242.232 kb on + strand, within GFF5225at 2242.233 kb on - strand, within GFF5225at 2242.571 kb on + strand, within GFF5225at 2242.571 kb on + strand, within GFF5225at 2242.572 kb on - strand, within GFF5225at 2242.572 kb on - strand, within GFF5225at 2242.782 kb on + strandat 2242.869 kb on + strandat 2242.870 kb on - strandat 2242.956 kb on + strand, within GFF5226at 2242.956 kb on + strand, within GFF5226at 2242.957 kb on - strand, within GFF5226at 2242.957 kb on - strand, within GFF5226at 2242.957 kb on - strand, within GFF5226at 2242.957 kb on - strand, within GFF5226at 2242.957 kb on - strand, within GFF5226at 2243.379 kb on + strand, within GFF5226at 2243.494 kb on - strand, within GFF5226at 2243.494 kb on - strand, within GFF5226at 2243.506 kb on - strand, within GFF5226at 2243.506 kb on - strand, within GFF5226at 2243.506 kb on - strand, within GFF5226at 2243.506 kb on - strand, within GFF5226at 2243.763 kb on + strandat 2243.763 kb on + strandat 2243.764 kb on - strand, within GFF5227at 2243.764 kb on - strand, within GFF5227at 2243.916 kb on - strand, within GFF5227at 2243.949 kb on - strand, within GFF5227at 2244.153 kb on - strand, within GFF5227at 2244.344 kb on + strand, within GFF5227at 2244.344 kb on + strand, within GFF5227at 2244.344 kb on + strand, within GFF5227at 2244.344 kb on + strand, within GFF5227at 2244.582 kb on + strandat 2244.583 kb on - strandat 2244.584 kb on + strandat 2244.584 kb on + strandat 2244.584 kb on + strandat 2244.585 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1
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2,242,012 + -1.0
2,242,012 + -0.2
2,242,045 + +0.2
2,242,046 - -0.3
2,242,046 - -0.0
2,242,046 - -0.2
2,242,046 - -0.2
2,242,100 - +0.4
2,242,100 - +0.0
2,242,100 - +0.2
2,242,100 - +0.9
2,242,100 - -0.2
2,242,100 - +0.2
2,242,115 + -0.1
2,242,115 + +0.2
2,242,232 + GFF5225 0.18 +0.2
2,242,233 - GFF5225 0.18 -0.3
2,242,571 + GFF5225 0.69 -0.7
2,242,571 + GFF5225 0.69 +1.2
2,242,572 - GFF5225 0.69 -0.6
2,242,572 - GFF5225 0.69 -0.9
2,242,782 + -0.7
2,242,869 + -2.2
2,242,870 - -0.4
2,242,956 + GFF5226 0.12 -1.2
2,242,956 + GFF5226 0.12 +0.4
2,242,957 - GFF5226 0.12 -2.3
2,242,957 - GFF5226 0.12 -0.7
2,242,957 - GFF5226 0.12 -1.4
2,242,957 - GFF5226 0.12 -0.7
2,242,957 - GFF5226 0.12 -0.2
2,243,379 + GFF5226 0.64 -1.4
2,243,494 - GFF5226 0.77 -3.2
2,243,494 - GFF5226 0.77 -1.2
2,243,506 - GFF5226 0.79 -2.2
2,243,506 - GFF5226 0.79 -0.0
2,243,506 - GFF5226 0.79 -1.8
2,243,506 - GFF5226 0.79 -2.1
2,243,763 + +0.3
2,243,763 + +0.5
2,243,764 - GFF5227 0.10 +0.3
2,243,764 - GFF5227 0.10 -0.1
2,243,916 - GFF5227 0.27 -0.7
2,243,949 - GFF5227 0.31 -0.8
2,244,153 - GFF5227 0.54 +0.9
2,244,344 + GFF5227 0.76 +0.8
2,244,344 + GFF5227 0.76 +0.2
2,244,344 + GFF5227 0.76 -0.2
2,244,344 + GFF5227 0.76 +0.2
2,244,582 + -1.4
2,244,583 - -0.7
2,244,584 + -0.5
2,244,584 + +0.1
2,244,584 + -0.8
2,244,585 - -0.1

Or see this region's nucleotide sequence