Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF180

Experiment: Ying_OrganicAcid7 rep C; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF178 and GFF179 are separated by 7 nucleotidesGFF179 and GFF180 are separated by 85 nucleotidesGFF180 and GFF181 overlap by 27 nucleotides GFF178 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 191,778 to 192,737 GFF178 GFF179 - Small-conductance mechanosensitive channel, at 192,745 to 193,320 GFF179 GFF180 - hypothetical protein, at 193,406 to 193,702 GFF180 GFF181 - Putative enzyme of poly-gamma-glutamate biosynthesis (capsule formation), at 193,676 to 195,007 GFF181 Position (kb) 193 194Strain fitness (log2 ratio) -2 -1 0 1 2at 192.634 kb on - strand, within GFF178at 192.876 kb on - strand, within GFF179at 193.502 kb on + strand, within GFF180at 193.503 kb on - strand, within GFF180at 193.503 kb on - strand, within GFF180at 193.757 kb on + strandat 194.021 kb on + strand, within GFF181at 194.022 kb on - strand, within GFF181at 194.156 kb on + strand, within GFF181at 194.156 kb on + strand, within GFF181at 194.156 kb on + strand, within GFF181at 194.436 kb on - strand, within GFF181at 194.436 kb on - strand, within GFF181at 194.525 kb on + strand, within GFF181at 194.525 kb on + strand, within GFF181at 194.525 kb on + strand, within GFF181at 194.526 kb on - strand, within GFF181at 194.526 kb on - strand, within GFF181

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1
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192,634 - GFF178 0.89 -1.1
192,876 - GFF179 0.23 +1.2
193,502 + GFF180 0.32 -0.1
193,503 - GFF180 0.33 -1.0
193,503 - GFF180 0.33 -0.0
193,757 + -2.1
194,021 + GFF181 0.26 -0.1
194,022 - GFF181 0.26 +0.4
194,156 + GFF181 0.36 -0.6
194,156 + GFF181 0.36 +0.0
194,156 + GFF181 0.36 -0.1
194,436 - GFF181 0.57 +2.2
194,436 - GFF181 0.57 +1.1
194,525 + GFF181 0.64 +0.4
194,525 + GFF181 0.64 -0.5
194,525 + GFF181 0.64 +0.1
194,526 - GFF181 0.64 +0.7
194,526 - GFF181 0.64 -0.0

Or see this region's nucleotide sequence