Experiment: Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS19915 and COO64_RS19920 overlap by 4 nucleotides COO64_RS19920 and COO64_RS19925 are separated by 59 nucleotides COO64_RS19925 and recA are separated by 102 nucleotides
COO64_RS19915: COO64_RS19915 - glycoside hydrolase family 19 protein, at 90,869 to 91,420
_RS19915
COO64_RS19920: COO64_RS19920 - lysis system i-spanin subunit Rz, at 91,417 to 91,917
_RS19920
COO64_RS19925: COO64_RS19925 - CinA family protein, at 91,977 to 92,459
_RS19925
COO64_RS19930: recA - recombinase RecA, at 92,562 to 93,629
recA
Position (kb) 91
92
93 Strain fitness (log2 ratio) -2
-1
0
1
2 at 91.032 kb on + strand, within COO64_RS19915 at 91.033 kb on - strand, within COO64_RS19915 at 91.033 kb on - strand, within COO64_RS19915 at 91.033 kb on - strand, within COO64_RS19915 at 91.033 kb on - strand, within COO64_RS19915 at 91.037 kb on + strand, within COO64_RS19915 at 91.037 kb on + strand, within COO64_RS19915 at 91.038 kb on - strand, within COO64_RS19915 at 91.038 kb on - strand, within COO64_RS19915 at 91.038 kb on - strand, within COO64_RS19915 at 91.038 kb on - strand, within COO64_RS19915 at 91.142 kb on + strand, within COO64_RS19915 at 91.184 kb on + strand, within COO64_RS19915 at 91.184 kb on + strand, within COO64_RS19915 at 91.185 kb on - strand, within COO64_RS19915 at 91.185 kb on - strand, within COO64_RS19915 at 91.185 kb on - strand, within COO64_RS19915 at 91.348 kb on + strand, within COO64_RS19915 at 91.348 kb on + strand, within COO64_RS19915 at 91.348 kb on + strand, within COO64_RS19915 at 91.348 kb on + strand, within COO64_RS19915 at 91.349 kb on - strand, within COO64_RS19915 at 91.349 kb on - strand, within COO64_RS19915 at 91.642 kb on + strand, within COO64_RS19920 at 91.642 kb on + strand, within COO64_RS19920 at 91.642 kb on + strand, within COO64_RS19920 at 91.642 kb on + strand, within COO64_RS19920 at 91.642 kb on + strand, within COO64_RS19920 at 91.643 kb on - strand, within COO64_RS19920 at 91.643 kb on - strand, within COO64_RS19920 at 91.643 kb on - strand, within COO64_RS19920 at 91.643 kb on - strand, within COO64_RS19920 at 91.643 kb on - strand, within COO64_RS19920 at 91.652 kb on + strand, within COO64_RS19920 at 91.652 kb on + strand, within COO64_RS19920 at 91.652 kb on + strand, within COO64_RS19920 at 91.652 kb on + strand, within COO64_RS19920 at 91.653 kb on - strand, within COO64_RS19920 at 91.799 kb on + strand, within COO64_RS19920 at 91.894 kb on + strand at 91.894 kb on + strand at 91.894 kb on + strand at 91.894 kb on + strand at 91.894 kb on + strand at 91.894 kb on + strand at 91.895 kb on - strand at 91.895 kb on - strand at 91.895 kb on - strand at 91.895 kb on - strand at 91.895 kb on - strand at 91.895 kb on - strand at 91.895 kb on - strand at 91.895 kb on - strand at 91.895 kb on - strand at 91.895 kb on - strand at 91.925 kb on + strand at 91.926 kb on - strand at 91.926 kb on - strand at 91.926 kb on - strand at 91.926 kb on - strand at 91.944 kb on + strand at 91.944 kb on + strand at 91.944 kb on + strand at 91.944 kb on + strand at 91.945 kb on - strand at 91.945 kb on - strand at 91.945 kb on - strand at 92.085 kb on - strand, within COO64_RS19925 at 92.128 kb on - strand, within COO64_RS19925 at 92.136 kb on + strand, within COO64_RS19925 at 92.136 kb on + strand, within COO64_RS19925 at 92.137 kb on - strand, within COO64_RS19925 at 92.329 kb on + strand, within COO64_RS19925 at 92.330 kb on - strand, within COO64_RS19925 at 92.330 kb on - strand, within COO64_RS19925 at 92.401 kb on + strand, within COO64_RS19925 at 92.416 kb on + strand at 92.429 kb on - strand at 92.440 kb on + strand at 92.441 kb on - strand at 92.441 kb on - strand at 92.441 kb on - strand at 92.441 kb on - strand at 92.441 kb on - strand at 92.509 kb on + strand at 92.513 kb on + strand at 92.514 kb on - strand at 92.514 kb on - strand at 92.547 kb on + strand at 92.547 kb on + strand at 92.547 kb on + strand at 92.548 kb on - strand at 92.752 kb on - strand, within recA at 92.771 kb on + strand, within recA at 92.822 kb on + strand, within recA at 92.822 kb on + strand, within recA at 92.865 kb on + strand, within recA at 92.865 kb on + strand, within recA at 93.086 kb on + strand, within recA at 93.194 kb on + strand, within recA at 93.210 kb on + strand, within recA at 93.210 kb on + strand, within recA at 93.210 kb on + strand, within recA at 93.210 kb on + strand, within recA at 93.210 kb on + strand, within recA at 93.210 kb on + strand, within recA at 93.211 kb on - strand, within recA at 93.211 kb on - strand, within recA at 93.211 kb on - strand, within recA at 93.211 kb on - strand, within recA at 93.211 kb on - strand, within recA at 93.211 kb on - strand, within recA at 93.211 kb on - strand, within recA at 93.211 kb on - strand, within recA at 93.211 kb on - strand, within recA at 93.348 kb on + strand, within recA at 93.348 kb on + strand, within recA at 93.363 kb on + strand, within recA at 93.364 kb on - strand, within recA at 93.429 kb on + strand, within recA at 93.430 kb on - strand, within recA at 93.435 kb on + strand, within recA at 93.435 kb on + strand, within recA at 93.436 kb on - strand, within recA
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in LB remove 91,032 + COO64_RS19915 0.30 +1.7 91,033 - COO64_RS19915 0.30 +1.7 91,033 - COO64_RS19915 0.30 +0.1 91,033 - COO64_RS19915 0.30 -0.3 91,033 - COO64_RS19915 0.30 -0.6 91,037 + COO64_RS19915 0.30 -0.7 91,037 + COO64_RS19915 0.30 +0.4 91,038 - COO64_RS19915 0.31 +0.5 91,038 - COO64_RS19915 0.31 +1.7 91,038 - COO64_RS19915 0.31 +0.2 91,038 - COO64_RS19915 0.31 +1.1 91,142 + COO64_RS19915 0.49 -0.1 91,184 + COO64_RS19915 0.57 +0.4 91,184 + COO64_RS19915 0.57 -1.3 91,185 - COO64_RS19915 0.57 +1.0 91,185 - COO64_RS19915 0.57 -0.4 91,185 - COO64_RS19915 0.57 -0.2 91,348 + COO64_RS19915 0.87 -0.0 91,348 + COO64_RS19915 0.87 +0.6 91,348 + COO64_RS19915 0.87 -0.3 91,348 + COO64_RS19915 0.87 -0.4 91,349 - COO64_RS19915 0.87 +0.5 91,349 - COO64_RS19915 0.87 +0.9 91,642 + COO64_RS19920 0.45 -0.8 91,642 + COO64_RS19920 0.45 +0.5 91,642 + COO64_RS19920 0.45 +0.1 91,642 + COO64_RS19920 0.45 +0.9 91,642 + COO64_RS19920 0.45 -0.5 91,643 - COO64_RS19920 0.45 -2.5 91,643 - COO64_RS19920 0.45 -0.8 91,643 - COO64_RS19920 0.45 -1.1 91,643 - COO64_RS19920 0.45 +0.2 91,643 - COO64_RS19920 0.45 +0.7 91,652 + COO64_RS19920 0.47 -0.8 91,652 + COO64_RS19920 0.47 -0.7 91,652 + COO64_RS19920 0.47 -0.6 91,652 + COO64_RS19920 0.47 +1.1 91,653 - COO64_RS19920 0.47 -0.3 91,799 + COO64_RS19920 0.76 +1.3 91,894 + +1.3 91,894 + -2.1 91,894 + -0.4 91,894 + -1.3 91,894 + -1.0 91,894 + -0.7 91,895 - -0.4 91,895 - -0.3 91,895 - -1.0 91,895 - -0.6 91,895 - +0.2 91,895 - +1.9 91,895 - -0.0 91,895 - +0.4 91,895 - +0.7 91,895 - +0.0 91,925 + +0.7 91,926 - +0.4 91,926 - -0.1 91,926 - -0.0 91,926 - -0.4 91,944 + -0.1 91,944 + -0.1 91,944 + +0.5 91,944 + -0.3 91,945 - +0.5 91,945 - +1.3 91,945 - +0.3 92,085 - COO64_RS19925 0.22 -0.3 92,128 - COO64_RS19925 0.31 -0.9 92,136 + COO64_RS19925 0.33 +2.7 92,136 + COO64_RS19925 0.33 -1.7 92,137 - COO64_RS19925 0.33 +0.9 92,329 + COO64_RS19925 0.73 -0.0 92,330 - COO64_RS19925 0.73 +0.6 92,330 - COO64_RS19925 0.73 +0.7 92,401 + COO64_RS19925 0.88 +0.9 92,416 + -0.3 92,429 - -0.2 92,440 + +0.0 92,441 - -0.6 92,441 - +0.4 92,441 - +0.1 92,441 - +1.0 92,441 - -1.3 92,509 + +0.7 92,513 + +0.5 92,514 - -0.3 92,514 - -1.3 92,547 + +1.2 92,547 + -0.8 92,547 + -2.1 92,548 - -0.9 92,752 - recA COO64_RS19930 0.18 -0.3 92,771 + recA COO64_RS19930 0.20 +0.9 92,822 + recA COO64_RS19930 0.24 -0.3 92,822 + recA COO64_RS19930 0.24 +0.8 92,865 + recA COO64_RS19930 0.28 +1.7 92,865 + recA COO64_RS19930 0.28 +0.4 93,086 + recA COO64_RS19930 0.49 -0.9 93,194 + recA COO64_RS19930 0.59 -0.3 93,210 + recA COO64_RS19930 0.61 -0.6 93,210 + recA COO64_RS19930 0.61 +0.9 93,210 + recA COO64_RS19930 0.61 -0.3 93,210 + recA COO64_RS19930 0.61 -1.2 93,210 + recA COO64_RS19930 0.61 +0.7 93,210 + recA COO64_RS19930 0.61 +0.7 93,211 - recA COO64_RS19930 0.61 -2.5 93,211 - recA COO64_RS19930 0.61 -0.3 93,211 - recA COO64_RS19930 0.61 -1.5 93,211 - recA COO64_RS19930 0.61 -0.9 93,211 - recA COO64_RS19930 0.61 +0.9 93,211 - recA COO64_RS19930 0.61 +0.1 93,211 - recA COO64_RS19930 0.61 +0.1 93,211 - recA COO64_RS19930 0.61 -0.3 93,211 - recA COO64_RS19930 0.61 -0.6 93,348 + recA COO64_RS19930 0.74 -0.6 93,348 + recA COO64_RS19930 0.74 -0.3 93,363 + recA COO64_RS19930 0.75 -1.1 93,364 - recA COO64_RS19930 0.75 -0.3 93,429 + recA COO64_RS19930 0.81 +0.8 93,430 - recA COO64_RS19930 0.81 -2.1 93,435 + recA COO64_RS19930 0.82 -1.5 93,435 + recA COO64_RS19930 0.82 +0.7 93,436 - recA COO64_RS19930 0.82 -0.6
Or see this region's nucleotide sequence