Strain Fitness in Pseudomonas sp. DMC3 around GFF75

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF73 and GFF74 overlap by 20 nucleotidesGFF74 and GFF75 overlap by 8 nucleotidesGFF75 and GFF76 are separated by 146 nucleotides GFF73 - Malonyl-[acyl-carrier protein] O-methyltransferase, at 84,028 to 84,849 GFF73 GFF74 - Pimeloyl-[acyl-carrier protein] methyl ester esterase, at 84,830 to 85,561 GFF74 GFF75 - 8-amino-7-oxononanoate synthase, at 85,554 to 86,732 GFF75 GFF76 - Biotin synthase, at 86,879 to 87,934 GFF76 Position (kb) 85 86 87Strain fitness (log2 ratio) -2 -1 0 1at 84.851 kb on + strandat 84.851 kb on + strandat 84.851 kb on + strandat 84.852 kb on - strandat 84.852 kb on - strandat 84.852 kb on - strandat 84.852 kb on - strandat 85.163 kb on + strand, within GFF74at 85.163 kb on + strand, within GFF74at 85.163 kb on + strand, within GFF74at 85.163 kb on + strand, within GFF74at 85.163 kb on + strand, within GFF74at 85.164 kb on - strand, within GFF74at 85.164 kb on - strand, within GFF74at 85.164 kb on - strand, within GFF74at 85.164 kb on - strand, within GFF74at 85.164 kb on - strand, within GFF74at 85.164 kb on - strand, within GFF74at 85.556 kb on - strandat 85.556 kb on - strandat 85.620 kb on + strandat 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.942 kb on + strand, within GFF75at 85.943 kb on - strand, within GFF75at 85.943 kb on - strand, within GFF75at 85.943 kb on - strand, within GFF75at 85.946 kb on + strand, within GFF75at 85.946 kb on + strand, within GFF75at 85.946 kb on + strand, within GFF75at 85.946 kb on + strand, within GFF75at 85.946 kb on + strand, within GFF75at 85.946 kb on + strand, within GFF75at 85.947 kb on - strand, within GFF75at 85.947 kb on - strand, within GFF75at 85.948 kb on + strand, within GFF75at 85.948 kb on + strand, within GFF75at 85.948 kb on + strand, within GFF75at 85.948 kb on + strand, within GFF75at 85.948 kb on + strand, within GFF75at 85.949 kb on - strand, within GFF75at 85.949 kb on - strand, within GFF75at 85.949 kb on - strand, within GFF75at 85.949 kb on - strand, within GFF75at 85.949 kb on - strand, within GFF75at 85.949 kb on - strand, within GFF75at 85.949 kb on - strand, within GFF75at 85.949 kb on - strand, within GFF75at 86.028 kb on - strand, within GFF75at 86.211 kb on + strand, within GFF75at 86.290 kb on + strand, within GFF75at 86.290 kb on + strand, within GFF75at 86.413 kb on + strand, within GFF75at 86.413 kb on + strand, within GFF75at 86.413 kb on + strand, within GFF75at 86.593 kb on + strand, within GFF75at 86.593 kb on + strand, within GFF75at 86.594 kb on - strand, within GFF75at 86.594 kb on - strand, within GFF75at 86.877 kb on + strandat 86.878 kb on - strandat 86.880 kb on + strandat 86.881 kb on - strandat 86.881 kb on - strandat 86.881 kb on - strandat 86.895 kb on + strandat 86.895 kb on + strandat 86.895 kb on + strandat 86.895 kb on + strandat 86.896 kb on - strandat 86.896 kb on - strandat 86.908 kb on + strandat 87.188 kb on - strand, within GFF76at 87.381 kb on + strand, within GFF76at 87.382 kb on - strand, within GFF76at 87.382 kb on - strand, within GFF76at 87.382 kb on - strand, within GFF76at 87.382 kb on - strand, within GFF76at 87.382 kb on - strand, within GFF76at 87.408 kb on + strand, within GFF76at 87.435 kb on + strand, within GFF76at 87.435 kb on + strand, within GFF76at 87.436 kb on - strand, within GFF76at 87.436 kb on - strand, within GFF76at 87.436 kb on - strand, within GFF76at 87.468 kb on + strand, within GFF76at 87.468 kb on + strand, within GFF76at 87.468 kb on + strand, within GFF76at 87.468 kb on + strand, within GFF76at 87.468 kb on + strand, within GFF76at 87.468 kb on + strand, within GFF76at 87.469 kb on - strand, within GFF76at 87.469 kb on - strand, within GFF76at 87.469 kb on - strand, within GFF76at 87.469 kb on - strand, within GFF76at 87.471 kb on + strand, within GFF76at 87.471 kb on + strand, within GFF76at 87.472 kb on - strand, within GFF76at 87.472 kb on - strand, within GFF76at 87.582 kb on + strand, within GFF76at 87.582 kb on + strand, within GFF76at 87.582 kb on + strand, within GFF76at 87.583 kb on - strand, within GFF76at 87.721 kb on - strand, within GFF76at 87.721 kb on - strand, within GFF76

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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84,851 + +1.3
84,851 + +0.4
84,851 + -1.5
84,852 - -0.9
84,852 - -0.4
84,852 - -0.9
84,852 - -0.4
85,163 + GFF74 0.45 +1.1
85,163 + GFF74 0.45 -0.1
85,163 + GFF74 0.45 -1.3
85,163 + GFF74 0.45 -0.0
85,163 + GFF74 0.45 -0.3
85,164 - GFF74 0.46 +0.2
85,164 - GFF74 0.46 +0.1
85,164 - GFF74 0.46 +0.3
85,164 - GFF74 0.46 +0.5
85,164 - GFF74 0.46 +1.1
85,164 - GFF74 0.46 -1.1
85,556 - -0.2
85,556 - -0.9
85,620 + +0.1
85,942 + GFF75 0.33 +1.0
85,942 + GFF75 0.33 -1.2
85,942 + GFF75 0.33 -0.6
85,942 + GFF75 0.33 +0.9
85,942 + GFF75 0.33 +0.0
85,942 + GFF75 0.33 -0.4
85,942 + GFF75 0.33 -0.4
85,942 + GFF75 0.33 +0.6
85,942 + GFF75 0.33 -1.4
85,942 + GFF75 0.33 -0.4
85,942 + GFF75 0.33 -0.3
85,943 - GFF75 0.33 -1.4
85,943 - GFF75 0.33 +0.6
85,943 - GFF75 0.33 +0.7
85,946 + GFF75 0.33 +0.4
85,946 + GFF75 0.33 +0.5
85,946 + GFF75 0.33 -1.6
85,946 + GFF75 0.33 +0.8
85,946 + GFF75 0.33 -0.4
85,946 + GFF75 0.33 -0.2
85,947 - GFF75 0.33 -0.1
85,947 - GFF75 0.33 -0.2
85,948 + GFF75 0.33 -0.5
85,948 + GFF75 0.33 +1.0
85,948 + GFF75 0.33 -0.1
85,948 + GFF75 0.33 -0.0
85,948 + GFF75 0.33 +0.3
85,949 - GFF75 0.34 +0.5
85,949 - GFF75 0.34 -0.2
85,949 - GFF75 0.34 +0.9
85,949 - GFF75 0.34 -0.2
85,949 - GFF75 0.34 -1.9
85,949 - GFF75 0.34 -0.0
85,949 - GFF75 0.34 +0.3
85,949 - GFF75 0.34 -0.4
86,028 - GFF75 0.40 -0.8
86,211 + GFF75 0.56 -0.4
86,290 + GFF75 0.62 -0.2
86,290 + GFF75 0.62 -0.0
86,413 + GFF75 0.73 +0.2
86,413 + GFF75 0.73 +0.6
86,413 + GFF75 0.73 +1.6
86,593 + GFF75 0.88 -0.7
86,593 + GFF75 0.88 +0.2
86,594 - GFF75 0.88 +0.0
86,594 - GFF75 0.88 -0.5
86,877 + +0.7
86,878 - +0.1
86,880 + -0.7
86,881 - +0.7
86,881 - -0.1
86,881 - -0.4
86,895 + -0.4
86,895 + -1.0
86,895 + +1.4
86,895 + +0.5
86,896 - +0.3
86,896 - +1.1
86,908 + +0.2
87,188 - GFF76 0.29 -2.0
87,381 + GFF76 0.48 -0.1
87,382 - GFF76 0.48 -1.1
87,382 - GFF76 0.48 +0.6
87,382 - GFF76 0.48 -0.3
87,382 - GFF76 0.48 -0.5
87,382 - GFF76 0.48 -1.8
87,408 + GFF76 0.50 +0.1
87,435 + GFF76 0.53 -0.5
87,435 + GFF76 0.53 -0.2
87,436 - GFF76 0.53 +1.6
87,436 - GFF76 0.53 -0.3
87,436 - GFF76 0.53 -1.1
87,468 + GFF76 0.56 +0.5
87,468 + GFF76 0.56 +0.7
87,468 + GFF76 0.56 +0.5
87,468 + GFF76 0.56 -0.5
87,468 + GFF76 0.56 -0.6
87,468 + GFF76 0.56 +0.1
87,469 - GFF76 0.56 -0.0
87,469 - GFF76 0.56 -0.5
87,469 - GFF76 0.56 -0.9
87,469 - GFF76 0.56 +0.6
87,471 + GFF76 0.56 -0.9
87,471 + GFF76 0.56 -0.5
87,472 - GFF76 0.56 -0.3
87,472 - GFF76 0.56 -0.6
87,582 + GFF76 0.67 +0.4
87,582 + GFF76 0.67 -1.6
87,582 + GFF76 0.67 -0.0
87,583 - GFF76 0.67 +1.1
87,721 - GFF76 0.80 -0.3
87,721 - GFF76 0.80 -0.5

Or see this region's nucleotide sequence