Strain Fitness in Pseudomonas sp. DMC3 around GFF216

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF214 and GFF215 overlap by 4 nucleotidesGFF215 and GFF216 overlap by 4 nucleotidesGFF216 and GFF217 are separated by 22 nucleotidesGFF217 and GFF218 are separated by 132 nucleotides GFF214 - hypothetical protein, at 243,479 to 243,913 GFF214 GFF215 - dITP/XTP pyrophosphatase, at 243,910 to 244,506 GFF215 GFF216 - Heme chaperone HemW, at 244,503 to 245,708 GFF216 GFF217 - hypothetical protein, at 245,731 to 246,054 GFF217 GFF218 - tRNA (guanine-N(7)-)-methyltransferase, at 246,187 to 246,912 GFF218 Position (kb) 244 245 246Strain fitness (log2 ratio) -1 0 1 2at 243.590 kb on + strand, within GFF214at 243.590 kb on + strand, within GFF214at 243.590 kb on + strand, within GFF214at 243.591 kb on - strand, within GFF214at 243.591 kb on - strand, within GFF214at 243.591 kb on - strand, within GFF214at 243.591 kb on - strand, within GFF214at 243.794 kb on + strand, within GFF214at 243.794 kb on + strand, within GFF214at 243.794 kb on + strand, within GFF214at 243.795 kb on - strand, within GFF214at 243.795 kb on - strand, within GFF214at 243.795 kb on - strand, within GFF214at 243.797 kb on + strand, within GFF214at 243.797 kb on + strand, within GFF214at 243.797 kb on + strand, within GFF214at 243.797 kb on + strand, within GFF214at 243.797 kb on + strand, within GFF214at 243.797 kb on + strand, within GFF214at 243.798 kb on - strand, within GFF214at 243.798 kb on - strand, within GFF214at 243.809 kb on + strand, within GFF214at 243.809 kb on + strand, within GFF214at 244.165 kb on + strand, within GFF215at 244.165 kb on + strand, within GFF215at 244.165 kb on + strand, within GFF215at 244.166 kb on - strand, within GFF215at 244.166 kb on - strand, within GFF215at 244.166 kb on - strand, within GFF215at 244.166 kb on - strand, within GFF215at 244.166 kb on - strand, within GFF215at 244.166 kb on - strand, within GFF215at 244.177 kb on + strand, within GFF215at 244.275 kb on - strand, within GFF215at 244.275 kb on - strand, within GFF215at 244.596 kb on + strandat 244.596 kb on + strandat 244.596 kb on + strandat 244.683 kb on + strand, within GFF216at 244.683 kb on + strand, within GFF216at 244.683 kb on + strand, within GFF216at 244.684 kb on - strand, within GFF216at 244.684 kb on - strand, within GFF216at 244.684 kb on - strand, within GFF216at 244.684 kb on - strand, within GFF216at 244.731 kb on + strand, within GFF216at 244.731 kb on + strand, within GFF216at 244.731 kb on + strand, within GFF216at 244.731 kb on + strand, within GFF216at 244.731 kb on + strand, within GFF216at 244.732 kb on - strand, within GFF216at 244.732 kb on - strand, within GFF216at 244.732 kb on - strand, within GFF216at 244.905 kb on + strand, within GFF216at 244.906 kb on - strand, within GFF216at 245.043 kb on - strand, within GFF216at 245.148 kb on + strand, within GFF216at 245.171 kb on + strand, within GFF216at 245.171 kb on + strand, within GFF216at 245.171 kb on + strand, within GFF216at 245.265 kb on + strand, within GFF216at 245.274 kb on + strand, within GFF216at 245.275 kb on - strand, within GFF216at 245.275 kb on - strand, within GFF216at 245.318 kb on + strand, within GFF216at 245.319 kb on - strand, within GFF216at 245.319 kb on - strand, within GFF216at 245.328 kb on + strand, within GFF216at 245.329 kb on - strand, within GFF216at 245.329 kb on - strand, within GFF216at 245.436 kb on + strand, within GFF216at 245.553 kb on + strand, within GFF216at 245.944 kb on + strand, within GFF217at 245.944 kb on + strand, within GFF217at 245.944 kb on + strand, within GFF217at 245.944 kb on + strand, within GFF217at 245.944 kb on + strand, within GFF217at 245.944 kb on + strand, within GFF217at 245.989 kb on + strand, within GFF217at 245.989 kb on + strand, within GFF217at 245.990 kb on - strand, within GFF217at 245.990 kb on - strand, within GFF217at 245.990 kb on - strand, within GFF217at 245.990 kb on - strand, within GFF217at 246.017 kb on + strand, within GFF217at 246.347 kb on + strand, within GFF218at 246.348 kb on - strand, within GFF218at 246.348 kb on - strand, within GFF218at 246.536 kb on + strand, within GFF218at 246.554 kb on + strand, within GFF218

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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243,590 + GFF214 0.26 -1.7
243,590 + GFF214 0.26 +0.1
243,590 + GFF214 0.26 -0.5
243,591 - GFF214 0.26 -0.5
243,591 - GFF214 0.26 -1.3
243,591 - GFF214 0.26 +0.1
243,591 - GFF214 0.26 -0.3
243,794 + GFF214 0.72 -0.1
243,794 + GFF214 0.72 -0.7
243,794 + GFF214 0.72 +0.3
243,795 - GFF214 0.73 +0.3
243,795 - GFF214 0.73 -0.3
243,795 - GFF214 0.73 -0.9
243,797 + GFF214 0.73 -0.1
243,797 + GFF214 0.73 +0.4
243,797 + GFF214 0.73 +0.1
243,797 + GFF214 0.73 -0.1
243,797 + GFF214 0.73 +0.1
243,797 + GFF214 0.73 -0.7
243,798 - GFF214 0.73 -1.2
243,798 - GFF214 0.73 +0.2
243,809 + GFF214 0.76 +0.6
243,809 + GFF214 0.76 +0.5
244,165 + GFF215 0.43 -0.1
244,165 + GFF215 0.43 +0.1
244,165 + GFF215 0.43 +1.1
244,166 - GFF215 0.43 -1.0
244,166 - GFF215 0.43 +0.2
244,166 - GFF215 0.43 -0.2
244,166 - GFF215 0.43 -0.8
244,166 - GFF215 0.43 -0.3
244,166 - GFF215 0.43 -0.7
244,177 + GFF215 0.45 -0.9
244,275 - GFF215 0.61 +0.4
244,275 - GFF215 0.61 -0.4
244,596 + -0.9
244,596 + +0.1
244,596 + -0.3
244,683 + GFF216 0.15 +0.3
244,683 + GFF216 0.15 +2.3
244,683 + GFF216 0.15 +0.3
244,684 - GFF216 0.15 +0.2
244,684 - GFF216 0.15 +0.9
244,684 - GFF216 0.15 -0.9
244,684 - GFF216 0.15 +0.4
244,731 + GFF216 0.19 +0.0
244,731 + GFF216 0.19 -0.0
244,731 + GFF216 0.19 +0.4
244,731 + GFF216 0.19 +0.1
244,731 + GFF216 0.19 +0.1
244,732 - GFF216 0.19 -0.9
244,732 - GFF216 0.19 -1.1
244,732 - GFF216 0.19 -0.8
244,905 + GFF216 0.33 -0.2
244,906 - GFF216 0.33 +0.4
245,043 - GFF216 0.45 +1.0
245,148 + GFF216 0.53 +1.0
245,171 + GFF216 0.55 -0.9
245,171 + GFF216 0.55 -0.8
245,171 + GFF216 0.55 -0.6
245,265 + GFF216 0.63 +0.4
245,274 + GFF216 0.64 -0.6
245,275 - GFF216 0.64 -1.3
245,275 - GFF216 0.64 -0.1
245,318 + GFF216 0.68 +0.1
245,319 - GFF216 0.68 -0.8
245,319 - GFF216 0.68 +0.9
245,328 + GFF216 0.68 -1.1
245,329 - GFF216 0.68 -0.2
245,329 - GFF216 0.68 +0.3
245,436 + GFF216 0.77 -0.5
245,553 + GFF216 0.87 -0.2
245,944 + GFF217 0.66 +0.9
245,944 + GFF217 0.66 +0.7
245,944 + GFF217 0.66 -0.4
245,944 + GFF217 0.66 +1.1
245,944 + GFF217 0.66 -0.4
245,944 + GFF217 0.66 +0.4
245,989 + GFF217 0.80 -0.6
245,989 + GFF217 0.80 -1.3
245,990 - GFF217 0.80 +0.0
245,990 - GFF217 0.80 -0.0
245,990 - GFF217 0.80 +0.7
245,990 - GFF217 0.80 +1.2
246,017 + GFF217 0.88 -0.4
246,347 + GFF218 0.22 -0.0
246,348 - GFF218 0.22 +0.4
246,348 - GFF218 0.22 -0.6
246,536 + GFF218 0.48 -0.9
246,554 + GFF218 0.51 -0.4

Or see this region's nucleotide sequence