Experiment: Time0_primer_test
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HUW76_07390 and HUW76_07395 overlap by 11 nucleotides HUW76_07395 and HUW76_07400 are separated by 176 nucleotides HUW76_07400 and HUW76_07405 are separated by 143 nucleotides HUW76_07405 and HUW76_07410 are separated by 17 nucleotides
HUW76_07390: HUW76_07390 - glutamate-1-semialdehyde 2,1-aminomutase, at 1,539,652 to 1,540,956
_07390
HUW76_07395: HUW76_07395 - bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase, at 1,540,946 to 1,541,398
_07395
HUW76_07400: HUW76_07400 - preprotein translocase subunit SecG, at 1,541,575 to 1,541,799
_07400
HUW76_07405: HUW76_07405 - glycerol-3-phosphate 1-O-acyltransferase PlsY, at 1,541,943 to 1,542,527
_07405
HUW76_07410: HUW76_07410 - DNA polymerase III subunit beta, at 1,542,545 to 1,543,690
_07410
Position (kb)
1541
1542 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1540.595 kb on - strand, within HUW76_07390 at 1540.595 kb on - strand, within HUW76_07390 at 1540.685 kb on - strand, within HUW76_07390 at 1540.759 kb on + strand, within HUW76_07390 at 1540.901 kb on + strand at 1540.901 kb on + strand at 1540.901 kb on + strand at 1540.901 kb on + strand at 1540.901 kb on - strand at 1540.901 kb on - strand at 1540.901 kb on - strand at 1540.916 kb on - strand at 1540.976 kb on + strand at 1541.205 kb on - strand, within HUW76_07395 at 1541.210 kb on - strand, within HUW76_07395 at 1541.210 kb on - strand, within HUW76_07395 at 1541.294 kb on - strand, within HUW76_07395 at 1541.406 kb on - strand at 1541.468 kb on + strand at 1541.474 kb on + strand at 1541.475 kb on + strand at 1541.572 kb on + strand at 1541.593 kb on - strand at 1541.641 kb on + strand, within HUW76_07400 at 1541.649 kb on - strand, within HUW76_07400 at 1541.687 kb on + strand, within HUW76_07400 at 1541.723 kb on + strand, within HUW76_07400 at 1541.780 kb on + strand at 1541.780 kb on + strand at 1541.833 kb on + strand at 1541.935 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_primer_test remove 1,540,595 - HUW76_07390 0.72 +2.0 1,540,595 - HUW76_07390 0.72 +2.0 1,540,685 - HUW76_07390 0.79 +1.1 1,540,759 + HUW76_07390 0.85 +0.1 1,540,901 + +0.7 1,540,901 + -1.4 1,540,901 + -0.4 1,540,901 + +0.6 1,540,901 - -0.5 1,540,901 - -2.5 1,540,901 - +0.8 1,540,916 - -1.0 1,540,976 + +0.8 1,541,205 - HUW76_07395 0.57 +0.6 1,541,210 - HUW76_07395 0.58 -0.4 1,541,210 - HUW76_07395 0.58 +0.8 1,541,294 - HUW76_07395 0.77 -0.0 1,541,406 - -0.3 1,541,468 + -0.2 1,541,474 + -0.3 1,541,475 + +0.0 1,541,572 + +0.2 1,541,593 - +0.7 1,541,641 + HUW76_07400 0.29 -0.1 1,541,649 - HUW76_07400 0.33 +0.1 1,541,687 + HUW76_07400 0.50 -0.2 1,541,723 + HUW76_07400 0.66 -2.2 1,541,780 + -2.0 1,541,780 + +0.3 1,541,833 + -0.3 1,541,935 + -0.8
Or see this region's nucleotide sequence