Experiment: Time0_primer_test
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HUW76_02045 and HUW76_02050 are separated by 5 nucleotides HUW76_02050 and HUW76_02055 are separated by 11 nucleotides
HUW76_02045: HUW76_02045 - response regulator, at 404,101 to 405,972
_02045
HUW76_02050: HUW76_02050 - response regulator transcription factor, at 405,978 to 406,649
_02050
HUW76_02055: HUW76_02055 - PocR ligand-binding domain-containing protein, at 406,661 to 407,458
_02055
Position (kb)
405
406
407 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 404.979 kb on - strand, within HUW76_02045 at 405.028 kb on + strand, within HUW76_02045 at 405.028 kb on + strand, within HUW76_02045 at 405.028 kb on + strand, within HUW76_02045 at 405.028 kb on + strand, within HUW76_02045 at 405.028 kb on + strand, within HUW76_02045 at 405.028 kb on + strand, within HUW76_02045 at 405.028 kb on + strand, within HUW76_02045 at 405.036 kb on - strand, within HUW76_02045 at 405.036 kb on - strand, within HUW76_02045 at 405.041 kb on + strand, within HUW76_02045 at 405.041 kb on + strand, within HUW76_02045 at 405.046 kb on - strand, within HUW76_02045 at 405.046 kb on - strand, within HUW76_02045 at 405.049 kb on - strand, within HUW76_02045 at 405.112 kb on + strand, within HUW76_02045 at 405.118 kb on + strand, within HUW76_02045 at 405.118 kb on + strand, within HUW76_02045 at 405.120 kb on - strand, within HUW76_02045 at 405.126 kb on - strand, within HUW76_02045 at 405.126 kb on - strand, within HUW76_02045 at 405.126 kb on - strand, within HUW76_02045 at 405.126 kb on - strand, within HUW76_02045 at 405.145 kb on - strand, within HUW76_02045 at 405.195 kb on + strand, within HUW76_02045 at 405.195 kb on + strand, within HUW76_02045 at 405.341 kb on + strand, within HUW76_02045 at 405.341 kb on + strand, within HUW76_02045 at 405.349 kb on - strand, within HUW76_02045 at 405.349 kb on - strand, within HUW76_02045 at 405.387 kb on + strand, within HUW76_02045 at 405.387 kb on + strand, within HUW76_02045 at 405.395 kb on - strand, within HUW76_02045 at 405.435 kb on - strand, within HUW76_02045 at 405.435 kb on - strand, within HUW76_02045 at 405.452 kb on - strand, within HUW76_02045 at 405.452 kb on - strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.508 kb on + strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.516 kb on - strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.518 kb on + strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.526 kb on - strand, within HUW76_02045 at 405.544 kb on - strand, within HUW76_02045 at 405.544 kb on - strand, within HUW76_02045 at 405.552 kb on + strand, within HUW76_02045 at 405.556 kb on - strand, within HUW76_02045 at 405.556 kb on - strand, within HUW76_02045 at 405.560 kb on - strand, within HUW76_02045 at 405.560 kb on - strand, within HUW76_02045 at 405.560 kb on - strand, within HUW76_02045 at 405.706 kb on + strand, within HUW76_02045 at 405.706 kb on + strand, within HUW76_02045 at 405.706 kb on + strand, within HUW76_02045 at 405.706 kb on + strand, within HUW76_02045 at 405.706 kb on + strand, within HUW76_02045 at 405.706 kb on + strand, within HUW76_02045 at 405.706 kb on + strand, within HUW76_02045 at 405.706 kb on + strand, within HUW76_02045 at 405.707 kb on + strand, within HUW76_02045 at 405.707 kb on + strand, within HUW76_02045 at 405.714 kb on - strand, within HUW76_02045 at 405.714 kb on - strand, within HUW76_02045 at 405.714 kb on - strand, within HUW76_02045 at 405.756 kb on + strand, within HUW76_02045 at 405.756 kb on + strand, within HUW76_02045 at 405.764 kb on - strand, within HUW76_02045 at 405.764 kb on - strand, within HUW76_02045 at 405.764 kb on - strand, within HUW76_02045 at 405.766 kb on + strand, within HUW76_02045 at 405.766 kb on + strand, within HUW76_02045 at 405.766 kb on + strand, within HUW76_02045 at 405.766 kb on + strand, within HUW76_02045 at 405.768 kb on + strand, within HUW76_02045 at 405.768 kb on + strand, within HUW76_02045 at 405.777 kb on + strand, within HUW76_02045 at 405.813 kb on + strand at 405.813 kb on + strand at 405.813 kb on + strand at 405.813 kb on + strand at 405.821 kb on - strand at 405.821 kb on - strand at 405.857 kb on - strand at 405.861 kb on - strand at 405.869 kb on - strand at 405.869 kb on - strand at 405.895 kb on - strand at 405.895 kb on - strand at 405.901 kb on - strand at 405.901 kb on - strand at 405.930 kb on + strand at 405.933 kb on - strand at 405.938 kb on - strand at 405.955 kb on - strand at 405.955 kb on - strand at 406.020 kb on + strand at 406.024 kb on + strand at 406.030 kb on + strand at 406.030 kb on + strand at 406.030 kb on + strand at 406.030 kb on + strand at 406.090 kb on + strand, within HUW76_02050 at 406.090 kb on + strand, within HUW76_02050 at 406.133 kb on + strand, within HUW76_02050 at 406.167 kb on - strand, within HUW76_02050 at 406.167 kb on - strand, within HUW76_02050 at 406.232 kb on + strand, within HUW76_02050 at 406.238 kb on - strand, within HUW76_02050 at 406.238 kb on - strand, within HUW76_02050 at 406.239 kb on + strand, within HUW76_02050 at 406.239 kb on + strand, within HUW76_02050 at 406.239 kb on + strand, within HUW76_02050 at 406.239 kb on + strand, within HUW76_02050 at 406.247 kb on - strand, within HUW76_02050 at 406.247 kb on - strand, within HUW76_02050 at 406.247 kb on - strand, within HUW76_02050 at 406.247 kb on - strand, within HUW76_02050 at 406.247 kb on - strand, within HUW76_02050 at 406.247 kb on - strand, within HUW76_02050 at 406.266 kb on + strand, within HUW76_02050 at 406.266 kb on + strand, within HUW76_02050 at 406.311 kb on + strand, within HUW76_02050 at 406.311 kb on + strand, within HUW76_02050 at 406.407 kb on + strand, within HUW76_02050 at 406.407 kb on + strand, within HUW76_02050 at 406.407 kb on + strand, within HUW76_02050 at 406.410 kb on + strand, within HUW76_02050 at 406.410 kb on + strand, within HUW76_02050 at 406.410 kb on + strand, within HUW76_02050 at 406.410 kb on + strand, within HUW76_02050 at 406.410 kb on + strand, within HUW76_02050 at 406.410 kb on + strand, within HUW76_02050 at 406.410 kb on + strand, within HUW76_02050 at 406.410 kb on + strand, within HUW76_02050 at 406.413 kb on - strand, within HUW76_02050 at 406.413 kb on - strand, within HUW76_02050 at 406.415 kb on - strand, within HUW76_02050 at 406.418 kb on - strand, within HUW76_02050 at 406.418 kb on - strand, within HUW76_02050 at 406.418 kb on - strand, within HUW76_02050 at 406.418 kb on - strand, within HUW76_02050 at 406.562 kb on - strand, within HUW76_02050 at 406.634 kb on - strand at 406.715 kb on + strand at 406.870 kb on + strand, within HUW76_02055 at 406.878 kb on - strand, within HUW76_02055 at 406.878 kb on - strand, within HUW76_02055 at 406.878 kb on - strand, within HUW76_02055 at 406.962 kb on + strand, within HUW76_02055 at 406.962 kb on + strand, within HUW76_02055 at 406.962 kb on + strand, within HUW76_02055 at 406.962 kb on + strand, within HUW76_02055 at 406.962 kb on + strand, within HUW76_02055 at 406.962 kb on + strand, within HUW76_02055 at 406.965 kb on + strand, within HUW76_02055 at 406.965 kb on + strand, within HUW76_02055 at 406.972 kb on + strand, within HUW76_02055 at 406.972 kb on + strand, within HUW76_02055 at 406.972 kb on + strand, within HUW76_02055 at 406.980 kb on - strand, within HUW76_02055 at 406.980 kb on - strand, within HUW76_02055 at 406.992 kb on + strand, within HUW76_02055 at 406.992 kb on + strand, within HUW76_02055 at 407.198 kb on + strand, within HUW76_02055 at 407.212 kb on + strand, within HUW76_02055 at 407.212 kb on + strand, within HUW76_02055 at 407.217 kb on + strand, within HUW76_02055 at 407.225 kb on - strand, within HUW76_02055 at 407.460 kb on - strand at 407.460 kb on - strand at 407.464 kb on - strand at 407.464 kb on - strand at 407.466 kb on + strand at 407.466 kb on + strand at 407.572 kb on - strand at 407.572 kb on - strand at 407.572 kb on - strand at 407.572 kb on - strand at 407.572 kb on - strand at 407.572 kb on - strand at 407.572 kb on - strand at 407.645 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_primer_test remove 404,979 - HUW76_02045 0.47 +1.4 405,028 + HUW76_02045 0.50 -0.3 405,028 + HUW76_02045 0.50 +0.7 405,028 + HUW76_02045 0.50 -0.1 405,028 + HUW76_02045 0.50 +0.1 405,028 + HUW76_02045 0.50 +0.7 405,028 + HUW76_02045 0.50 +0.4 405,028 + HUW76_02045 0.50 -0.5 405,036 - HUW76_02045 0.50 +2.0 405,036 - HUW76_02045 0.50 +1.5 405,041 + HUW76_02045 0.50 +0.1 405,041 + HUW76_02045 0.50 +0.4 405,046 - HUW76_02045 0.50 +0.8 405,046 - HUW76_02045 0.50 -0.2 405,049 - HUW76_02045 0.51 +0.3 405,112 + HUW76_02045 0.54 +0.1 405,118 + HUW76_02045 0.54 +0.3 405,118 + HUW76_02045 0.54 +0.2 405,120 - HUW76_02045 0.54 +1.6 405,126 - HUW76_02045 0.55 +0.2 405,126 - HUW76_02045 0.55 +1.3 405,126 - HUW76_02045 0.55 +0.5 405,126 - HUW76_02045 0.55 +0.8 405,145 - HUW76_02045 0.56 +0.5 405,195 + HUW76_02045 0.58 -0.5 405,195 + HUW76_02045 0.58 +0.3 405,341 + HUW76_02045 0.66 +0.1 405,341 + HUW76_02045 0.66 +0.0 405,349 - HUW76_02045 0.67 +2.0 405,349 - HUW76_02045 0.67 +1.1 405,387 + HUW76_02045 0.69 -1.2 405,387 + HUW76_02045 0.69 +0.1 405,395 - HUW76_02045 0.69 +1.5 405,435 - HUW76_02045 0.71 +2.9 405,435 - HUW76_02045 0.71 -0.8 405,452 - HUW76_02045 0.72 -0.1 405,452 - HUW76_02045 0.72 +1.3 405,508 + HUW76_02045 0.75 +0.9 405,508 + HUW76_02045 0.75 +0.4 405,508 + HUW76_02045 0.75 -0.0 405,508 + HUW76_02045 0.75 +0.4 405,508 + HUW76_02045 0.75 -0.1 405,508 + HUW76_02045 0.75 +0.4 405,508 + HUW76_02045 0.75 +0.1 405,508 + HUW76_02045 0.75 +0.2 405,508 + HUW76_02045 0.75 +0.4 405,508 + HUW76_02045 0.75 +0.7 405,508 + HUW76_02045 0.75 +0.5 405,508 + HUW76_02045 0.75 +0.3 405,508 + HUW76_02045 0.75 +0.2 405,508 + HUW76_02045 0.75 +0.2 405,508 + HUW76_02045 0.75 +0.3 405,508 + HUW76_02045 0.75 +0.1 405,508 + HUW76_02045 0.75 +0.6 405,508 + HUW76_02045 0.75 +0.0 405,508 + HUW76_02045 0.75 +0.2 405,508 + HUW76_02045 0.75 -0.1 405,508 + HUW76_02045 0.75 +0.0 405,508 + HUW76_02045 0.75 +0.0 405,508 + HUW76_02045 0.75 -0.1 405,508 + HUW76_02045 0.75 -0.1 405,508 + HUW76_02045 0.75 +0.4 405,508 + HUW76_02045 0.75 -0.5 405,508 + HUW76_02045 0.75 +0.4 405,516 - HUW76_02045 0.76 +1.8 405,516 - HUW76_02045 0.76 +0.9 405,516 - HUW76_02045 0.76 +0.6 405,516 - HUW76_02045 0.76 +1.4 405,516 - HUW76_02045 0.76 +0.9 405,516 - HUW76_02045 0.76 +2.0 405,516 - HUW76_02045 0.76 +1.4 405,516 - HUW76_02045 0.76 +1.4 405,516 - HUW76_02045 0.76 +1.7 405,516 - HUW76_02045 0.76 +0.7 405,516 - HUW76_02045 0.76 +0.8 405,516 - HUW76_02045 0.76 +1.2 405,516 - HUW76_02045 0.76 +1.6 405,516 - HUW76_02045 0.76 +1.1 405,516 - HUW76_02045 0.76 +4.9 405,516 - HUW76_02045 0.76 +0.4 405,516 - HUW76_02045 0.76 +0.4 405,518 + HUW76_02045 0.76 -0.3 405,518 + HUW76_02045 0.76 +0.9 405,518 + HUW76_02045 0.76 +0.8 405,518 + HUW76_02045 0.76 -0.1 405,518 + HUW76_02045 0.76 -0.2 405,518 + HUW76_02045 0.76 +0.2 405,518 + HUW76_02045 0.76 +1.1 405,518 + HUW76_02045 0.76 -0.3 405,518 + HUW76_02045 0.76 +0.6 405,518 + HUW76_02045 0.76 +0.5 405,518 + HUW76_02045 0.76 +0.2 405,526 - HUW76_02045 0.76 +1.0 405,526 - HUW76_02045 0.76 +0.7 405,526 - HUW76_02045 0.76 +0.3 405,526 - HUW76_02045 0.76 +0.8 405,526 - HUW76_02045 0.76 +0.3 405,526 - HUW76_02045 0.76 +0.4 405,526 - HUW76_02045 0.76 +0.5 405,526 - HUW76_02045 0.76 +0.2 405,526 - HUW76_02045 0.76 +0.6 405,526 - HUW76_02045 0.76 +0.0 405,526 - HUW76_02045 0.76 +0.6 405,526 - HUW76_02045 0.76 +0.5 405,544 - HUW76_02045 0.77 +1.3 405,544 - HUW76_02045 0.77 +0.4 405,552 + HUW76_02045 0.78 +0.6 405,556 - HUW76_02045 0.78 +0.8 405,556 - HUW76_02045 0.78 +0.5 405,560 - HUW76_02045 0.78 -0.3 405,560 - HUW76_02045 0.78 +0.9 405,560 - HUW76_02045 0.78 +1.8 405,706 + HUW76_02045 0.86 +0.2 405,706 + HUW76_02045 0.86 +1.2 405,706 + HUW76_02045 0.86 +0.3 405,706 + HUW76_02045 0.86 +0.5 405,706 + HUW76_02045 0.86 +0.3 405,706 + HUW76_02045 0.86 +1.8 405,706 + HUW76_02045 0.86 +0.5 405,706 + HUW76_02045 0.86 +0.0 405,707 + HUW76_02045 0.86 +0.6 405,707 + HUW76_02045 0.86 +0.0 405,714 - HUW76_02045 0.86 +2.0 405,714 - HUW76_02045 0.86 +1.6 405,714 - HUW76_02045 0.86 +3.2 405,756 + HUW76_02045 0.88 +0.4 405,756 + HUW76_02045 0.88 +0.0 405,764 - HUW76_02045 0.89 +2.1 405,764 - HUW76_02045 0.89 -2.3 405,764 - HUW76_02045 0.89 +0.9 405,766 + HUW76_02045 0.89 +0.3 405,766 + HUW76_02045 0.89 -0.1 405,766 + HUW76_02045 0.89 +0.7 405,766 + HUW76_02045 0.89 -0.2 405,768 + HUW76_02045 0.89 +0.4 405,768 + HUW76_02045 0.89 -1.0 405,777 + HUW76_02045 0.90 +0.5 405,813 + +0.2 405,813 + -0.3 405,813 + +0.3 405,813 + +0.1 405,821 - +2.0 405,821 - +1.2 405,857 - +1.8 405,861 - +1.7 405,869 - +1.5 405,869 - +0.1 405,895 - +2.0 405,895 - -0.4 405,901 - +1.4 405,901 - +1.0 405,930 + +2.8 405,933 - +1.2 405,938 - +0.7 405,955 - +1.6 405,955 - +0.7 406,020 + +0.6 406,024 + +0.1 406,030 + -0.6 406,030 + +0.3 406,030 + -0.1 406,030 + +0.2 406,090 + HUW76_02050 0.17 +0.3 406,090 + HUW76_02050 0.17 +0.2 406,133 + HUW76_02050 0.23 +0.3 406,167 - HUW76_02050 0.28 +1.0 406,167 - HUW76_02050 0.28 +1.0 406,232 + HUW76_02050 0.38 +0.2 406,238 - HUW76_02050 0.39 +3.5 406,238 - HUW76_02050 0.39 +3.2 406,239 + HUW76_02050 0.39 -0.0 406,239 + HUW76_02050 0.39 +0.9 406,239 + HUW76_02050 0.39 +0.1 406,239 + HUW76_02050 0.39 -0.1 406,247 - HUW76_02050 0.40 +2.6 406,247 - HUW76_02050 0.40 +3.9 406,247 - HUW76_02050 0.40 +0.8 406,247 - HUW76_02050 0.40 +1.0 406,247 - HUW76_02050 0.40 +0.9 406,247 - HUW76_02050 0.40 +1.7 406,266 + HUW76_02050 0.43 -0.5 406,266 + HUW76_02050 0.43 +0.1 406,311 + HUW76_02050 0.50 +1.5 406,311 + HUW76_02050 0.50 +2.3 406,407 + HUW76_02050 0.64 +0.3 406,407 + HUW76_02050 0.64 -0.1 406,407 + HUW76_02050 0.64 +0.2 406,410 + HUW76_02050 0.64 -0.0 406,410 + HUW76_02050 0.64 +0.1 406,410 + HUW76_02050 0.64 +0.3 406,410 + HUW76_02050 0.64 +0.4 406,410 + HUW76_02050 0.64 +0.9 406,410 + HUW76_02050 0.64 +0.3 406,410 + HUW76_02050 0.64 +0.1 406,410 + HUW76_02050 0.64 +0.4 406,413 - HUW76_02050 0.65 +1.7 406,413 - HUW76_02050 0.65 +1.9 406,415 - HUW76_02050 0.65 +1.9 406,418 - HUW76_02050 0.65 +0.6 406,418 - HUW76_02050 0.65 +2.0 406,418 - HUW76_02050 0.65 +0.5 406,418 - HUW76_02050 0.65 +1.1 406,562 - HUW76_02050 0.87 +0.9 406,634 - +1.3 406,715 + -0.2 406,870 + HUW76_02055 0.26 +0.6 406,878 - HUW76_02055 0.27 +1.2 406,878 - HUW76_02055 0.27 +1.2 406,878 - HUW76_02055 0.27 +1.0 406,962 + HUW76_02055 0.38 +0.5 406,962 + HUW76_02055 0.38 -0.1 406,962 + HUW76_02055 0.38 -0.2 406,962 + HUW76_02055 0.38 -0.2 406,962 + HUW76_02055 0.38 +0.4 406,962 + HUW76_02055 0.38 +0.3 406,965 + HUW76_02055 0.38 -0.1 406,965 + HUW76_02055 0.38 -0.3 406,972 + HUW76_02055 0.39 -0.2 406,972 + HUW76_02055 0.39 +0.2 406,972 + HUW76_02055 0.39 -0.3 406,980 - HUW76_02055 0.40 +5.2 406,980 - HUW76_02055 0.40 +2.3 406,992 + HUW76_02055 0.41 -0.2 406,992 + HUW76_02055 0.41 -0.2 407,198 + HUW76_02055 0.67 -0.1 407,212 + HUW76_02055 0.69 +0.4 407,212 + HUW76_02055 0.69 -0.3 407,217 + HUW76_02055 0.70 +0.2 407,225 - HUW76_02055 0.71 +2.0 407,460 - +0.4 407,460 - +0.4 407,464 - +2.4 407,464 - +1.3 407,466 + -0.2 407,466 + +0.3 407,572 - +1.4 407,572 - -0.9 407,572 - +1.0 407,572 - +0.4 407,572 - +0.1 407,572 - +1.6 407,572 - +0.4 407,645 + -0.3
Or see this region's nucleotide sequence