Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF421

Experiment: Ying_OrganicAcid7 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF420 and GFF421 are separated by 2 nucleotidesGFF421 and GFF422 are separated by 29 nucleotidesGFF422 and GFF423 overlap by 4 nucleotides GFF420 - Salicylate hydroxylase (EC 1.14.13.1), at 90,943 to 92,514 GFF420 GFF421 - Fumarylacetoacetate hydrolase family protein, at 92,517 to 93,383 GFF421 GFF422 - putative Metapyrocatechase (MPC) (CatO2ase) (Catechol 2,3- dioxygenase)( EC:1.13.11.2 ), at 93,413 to 94,318 GFF422 GFF423 - 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase (EC 4.1.1.45), at 94,315 to 95,364 GFF423 Position (kb) 92 93 94Strain fitness (log2 ratio) -1 0 1 2at 92.276 kb on + strand, within GFF420at 92.414 kb on + strandat 92.414 kb on + strandat 92.414 kb on + strandat 92.414 kb on + strandat 92.415 kb on - strandat 92.472 kb on - strandat 92.929 kb on + strand, within GFF421at 92.929 kb on + strand, within GFF421at 93.089 kb on - strand, within GFF421at 93.131 kb on - strand, within GFF421at 93.368 kb on - strandat 93.368 kb on - strandat 93.540 kb on + strand, within GFF422at 93.574 kb on - strand, within GFF422at 94.191 kb on + strand, within GFF422at 94.192 kb on - strand, within GFF422

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 1
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92,276 + GFF420 0.85 -0.2
92,414 + +0.3
92,414 + -0.2
92,414 + -0.3
92,414 + -1.1
92,415 - +0.2
92,472 - -1.3
92,929 + GFF421 0.48 -0.3
92,929 + GFF421 0.48 -0.1
93,089 - GFF421 0.66 +1.9
93,131 - GFF421 0.71 +1.0
93,368 - -0.6
93,368 - +0.3
93,540 + GFF422 0.14 +0.0
93,574 - GFF422 0.18 -0.7
94,191 + GFF422 0.86 -0.0
94,192 - GFF422 0.86 +0.5

Or see this region's nucleotide sequence