Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF3141

Experiment: Ying_OrganicAcid7 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3139 and GFF3140 overlap by 4 nucleotidesGFF3140 and GFF3141 overlap by 4 nucleotidesGFF3141 and GFF3142 are separated by 215 nucleotidesGFF3142 and GFF3143 overlap by 4 nucleotides GFF3139 - IncF plasmid conjugative transfer pilus assembly protein TraU, at 18,217 to 19,236 GFF3139 GFF3140 - IncF plasmid conjugative transfer pilus assembly protein TraW, at 19,233 to 19,883 GFF3140 GFF3141 - hypothetical protein, at 19,880 to 20,110 GFF3141 GFF3142 - Conjugative signal peptidase TrhF, at 20,326 to 20,937 GFF3142 GFF3143 - hypothetical protein, at 20,934 to 21,296 GFF3143 Position (kb) 19 20 21Strain fitness (log2 ratio) -1 0 1 2at 19.242 kb on + strandat 19.342 kb on + strand, within GFF3140at 19.343 kb on - strand, within GFF3140at 19.343 kb on - strand, within GFF3140at 19.343 kb on - strand, within GFF3140at 19.582 kb on + strand, within GFF3140at 19.583 kb on - strand, within GFF3140at 19.583 kb on - strand, within GFF3140at 20.064 kb on + strand, within GFF3141at 20.065 kb on - strand, within GFF3141at 20.065 kb on - strand, within GFF3141at 20.426 kb on + strand, within GFF3142at 20.427 kb on - strand, within GFF3142at 20.429 kb on + strand, within GFF3142at 20.430 kb on - strand, within GFF3142at 20.801 kb on + strand, within GFF3142at 20.801 kb on + strand, within GFF3142

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 1
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19,242 + +0.0
19,342 + GFF3140 0.17 -0.1
19,343 - GFF3140 0.17 +0.7
19,343 - GFF3140 0.17 -0.8
19,343 - GFF3140 0.17 +0.7
19,582 + GFF3140 0.54 +0.8
19,583 - GFF3140 0.54 +0.0
19,583 - GFF3140 0.54 -0.1
20,064 + GFF3141 0.80 -0.1
20,065 - GFF3141 0.80 +0.3
20,065 - GFF3141 0.80 +1.3
20,426 + GFF3142 0.16 +1.1
20,427 - GFF3142 0.17 +0.5
20,429 + GFF3142 0.17 +0.9
20,430 - GFF3142 0.17 +2.1
20,801 + GFF3142 0.78 -0.2
20,801 + GFF3142 0.78 +0.0

Or see this region's nucleotide sequence